Gene description for CPT1A
Gene name carnitine palmitoyltransferase 1A (liver)
Gene symbol CPT1A
Other names/aliases CPT1
CPT1-L
L-CPT1
Species Homo sapiens
 Database cross references - CPT1A
ExoCarta ExoCarta_1374
Vesiclepedia VP_1374
Entrez Gene 1374
HGNC 2328
MIM 600528
UniProt P50416  
 CPT1A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for CPT1A
Molecular Function
    carnitine O-palmitoyltransferase activity GO:0004095 IBA
    carnitine O-palmitoyltransferase activity GO:0004095 IDA
    carnitine O-palmitoyltransferase activity GO:0004095 IMP
    carnitine O-palmitoyltransferase activity GO:0004095 TAS
    protein-macromolecule adaptor activity GO:0030674 IDA
    identical protein binding GO:0042802 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    long-chain fatty acid metabolic process GO:0001676 IDA
    glucose metabolic process GO:0006006 IEA
    fatty acid metabolic process GO:0006631 IBA
    fatty acid beta-oxidation GO:0006635 IEA
    triglyceride metabolic process GO:0006641 IEA
    carnitine shuttle GO:0006853 TAS
    response to nutrient GO:0007584 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    carnitine metabolic process GO:0009437 IBA
    carnitine metabolic process GO:0009437 IDA
    regulation of lipid storage GO:0010883 IEA
    response to organic cyclic compound GO:0014070 IEA
    epithelial cell differentiation GO:0030855 IEP
    positive regulation of fatty acid beta-oxidation GO:0032000 IEA
    eating behavior GO:0042755 IEA
    response to alkaloid GO:0043279 IEA
    positive regulation of innate immune response GO:0045089 IDA
    response to ethanol GO:0045471 IEA
    aflatoxin metabolic process GO:0046222 IEA
    regulation of insulin secretion GO:0050796 IEA
    cellular response to fatty acid GO:0071398 IEA
    liver regeneration GO:0097421 IEA
    response to tetrachloromethane GO:1904772 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IMP
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial outer membrane GO:0005741 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified CPT1A in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CPT1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EMC4 51234
Co-fractionation Homo sapiens
2 ATP6V1B2 526
Co-fractionation Homo sapiens
3 LDHA 3939
Affinity Capture-Western Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 NDUFS1 4719
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
7 EIF3C 8663
Co-fractionation Homo sapiens
8 ATP6V1C1 528
Co-fractionation Homo sapiens
9 TMEM184A  
Affinity Capture-MS Homo sapiens
10 IGHM 3507
Affinity Capture-MS Homo sapiens
11 SLC2A1 6513
Affinity Capture-MS Homo sapiens
12 UBC 7316
Reconstituted Complex Homo sapiens
13 NDUFA4 4697
Affinity Capture-MS Homo sapiens
14 GABRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NDUFB9 4715
Co-fractionation Homo sapiens
16 PLOD3 8985
Co-fractionation Homo sapiens
17 Kcnk1  
Affinity Capture-MS Mus musculus
18 B3GNT2 10678
Affinity Capture-MS Homo sapiens
19 PCK1 5105
Affinity Capture-MS Homo sapiens
20 COQ9  
Affinity Capture-MS Homo sapiens
21 YIPF3 25844
Co-fractionation Homo sapiens
22 RHOA 387
Co-fractionation Homo sapiens
23 LPP 4026
Co-fractionation Homo sapiens
24 NR4A1  
Two-hybrid Homo sapiens
25 NDUFV1 4723
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
26 ATG16L1 55054
Affinity Capture-MS Homo sapiens
27 TOMM40 10452
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
28 TMEM59 9528
Affinity Capture-MS Homo sapiens
29 NDUFS5 4725
Co-fractionation Homo sapiens
30 KIF14 9928
Affinity Capture-MS Homo sapiens
31 ATP1A3 478
Affinity Capture-MS Homo sapiens
32 ITLN1 55600
Affinity Capture-MS Homo sapiens
33 KCNK1  
Affinity Capture-MS Homo sapiens
34 MTCH2 23788
Co-fractionation Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 Ppp4c 56420
Affinity Capture-MS Mus musculus
38 NDUFA8 4702
Co-fractionation Homo sapiens
39 SLC39A9 55334
Affinity Capture-MS Homo sapiens
40 CPS1 1373
Co-fractionation Homo sapiens
41 VSIG4  
Affinity Capture-MS Homo sapiens
42 SNRNP200 23020
Co-fractionation Homo sapiens
43 PLD3 23646
Affinity Capture-MS Homo sapiens
44 CYBA 1535
Two-hybrid Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 NPTN 27020
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 NDUFA2 4695
Co-fractionation Homo sapiens
49 CYC1 1537
Co-fractionation Homo sapiens
50 UQCRC1 7384
Co-fractionation Homo sapiens
51 RPN1 6184
Co-fractionation Homo sapiens
52 VDAC2 7417
Co-fractionation Homo sapiens
53 PCDHGC5  
Affinity Capture-MS Homo sapiens
54 ATP5B 506
Co-fractionation Homo sapiens
55 PHB 5245
Co-fractionation Homo sapiens
56 HSPA5 3309
Two-hybrid Homo sapiens
57 RAMP2  
Affinity Capture-MS Homo sapiens
58 CLN3 1201
Affinity Capture-MS Homo sapiens
59 HADHB 3032
Co-fractionation Homo sapiens
60 HADHA 3030
Co-fractionation Homo sapiens
61 TTYH1  
Affinity Capture-MS Homo sapiens
62 NPDC1  
Two-hybrid Homo sapiens
63 SDHA 6389
Affinity Capture-MS Homo sapiens
64 DUOXA2  
Affinity Capture-MS Homo sapiens
65 Wdr5  
Affinity Capture-MS Mus musculus
66 UQCRC2 7385
Co-fractionation Homo sapiens
67 HMOX2 3163
Co-fractionation Homo sapiens
68 KLRD1  
Affinity Capture-MS Homo sapiens
69 FBXO6 26270
Affinity Capture-MS Homo sapiens
70 AQP3  
Affinity Capture-MS Homo sapiens
71 KBTBD7  
Two-hybrid Homo sapiens
72 RIPK4  
Affinity Capture-MS Homo sapiens
73 ADAM7  
Affinity Capture-MS Homo sapiens
74 NXF1 10482
Affinity Capture-RNA Homo sapiens
75 EMC3 55831
Co-fractionation Homo sapiens
76 TSPAN8 7103
Affinity Capture-MS Homo sapiens
77 PCBP1 5093
Co-fractionation Homo sapiens
78 ATP2A1 487
Affinity Capture-MS Homo sapiens
79 EMC2 9694
Co-fractionation Homo sapiens
80 GPR45  
Affinity Capture-MS Homo sapiens
81 CMTM5  
Affinity Capture-MS Homo sapiens
82 ENO1 2023
Affinity Capture-RNA Homo sapiens
83 RNF2  
Affinity Capture-MS Homo sapiens
84 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
85 HSPD1 3329
Affinity Capture-MS Homo sapiens
86 SLC22A4 6583
Affinity Capture-MS Homo sapiens
87 CORO1C 23603
Co-fractionation Homo sapiens
88 HUWE1 10075
Affinity Capture-MS Homo sapiens
89 ZRANB1 54764
Affinity Capture-MS Homo sapiens
90 MAGEA8  
Affinity Capture-MS Homo sapiens
91 Tor1aip1  
Affinity Capture-MS Mus musculus
92 PHB2 11331
Co-fractionation Homo sapiens
93 CLIC1 1192
Two-hybrid Homo sapiens
94 USP50  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
95 RRBP1 6238
Co-fractionation Homo sapiens
96 TP53 7157
Affinity Capture-MS Homo sapiens
97 GP9 2815
Affinity Capture-MS Homo sapiens
98 ERLIN1 10613
Co-fractionation Homo sapiens
99 TUBG1 7283
Affinity Capture-MS Homo sapiens
100 MFSD4  
Affinity Capture-MS Homo sapiens
101 TBC1D9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 HDAC6 10013
Affinity Capture-MS Homo sapiens
103 BCKDHA 593
Co-fractionation Homo sapiens
104 PDHA1 5160
Affinity Capture-MS Homo sapiens
105 CD1A 909
Affinity Capture-MS Homo sapiens
106 NDUFS7 374291
Co-fractionation Homo sapiens
107 IFNLR1  
Affinity Capture-MS Homo sapiens
108 NDUFS8 4728
Co-fractionation Homo sapiens
109 MOV10 4343
Affinity Capture-RNA Homo sapiens
110 RAB7A 7879
Affinity Capture-MS Homo sapiens
111 ATP2B2 491
Affinity Capture-MS Homo sapiens
112 COX7C 1350
Co-fractionation Homo sapiens
113 C9orf72  
Affinity Capture-MS Homo sapiens
114 SLC1A1 6505
Affinity Capture-MS Homo sapiens
115 PLD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
116 TOMM5  
Co-fractionation Homo sapiens
117 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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