Gene description for GP9
Gene name glycoprotein IX (platelet)
Gene symbol GP9
Other names/aliases CD42a
GPIX
Species Homo sapiens
 Database cross references - GP9
ExoCarta ExoCarta_2815
Vesiclepedia VP_2815
Entrez Gene 2815
HGNC 4444
MIM 173515
UniProt P14770  
 GP9 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
 Gene ontology annotations for GP9
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    blood coagulation GO:0007596 TAS
    blood coagulation, intrinsic pathway GO:0007597 IPI
    blood coagulation, intrinsic pathway GO:0007597 NAS
    positive regulation of platelet activation GO:0010572 IDA
    positive regulation of platelet activation GO:0010572 NAS
    megakaryocyte development GO:0035855 ISO
    megakaryocyte development GO:0035855 NAS
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    release of sequestered calcium ion into cytosol GO:0051209 NAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    glycoprotein Ib-IX-V complex GO:1990779 IPI
    glycoprotein Ib-IX-V complex GO:1990779 NAS
 Experiment description of studies that identified GP9 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 274
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for GP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPY19L1 23333
Affinity Capture-MS Homo sapiens
2 DPY19L4  
Affinity Capture-MS Homo sapiens
3 SCNN1A  
Affinity Capture-MS Homo sapiens
4 C3orf58 205428
Affinity Capture-MS Homo sapiens
5 ATP13A2  
Affinity Capture-MS Homo sapiens
6 TM9SF2 9375
Affinity Capture-MS Homo sapiens
7 LPHN1  
Affinity Capture-MS Homo sapiens
8 MAN1B1 11253
Affinity Capture-MS Homo sapiens
9 BSCL2  
Affinity Capture-MS Homo sapiens
10 SRPR 6734
Affinity Capture-MS Homo sapiens
11 GLB1L2  
Affinity Capture-MS Homo sapiens
12 TMEM67 91147
Affinity Capture-MS Homo sapiens
13 GRAMD1A  
Affinity Capture-MS Homo sapiens
14 GPR110  
Affinity Capture-MS Homo sapiens
15 CPT1A 1374
Affinity Capture-MS Homo sapiens
16 ITFG1 81533
Affinity Capture-MS Homo sapiens
17 ADGRG1 9289
Affinity Capture-MS Homo sapiens
18 ASIC1  
Affinity Capture-MS Homo sapiens
19 ABHD3  
Affinity Capture-MS Homo sapiens
20 TM9SF4 9777
Affinity Capture-MS Homo sapiens
21 CUX1 1523
Affinity Capture-MS Homo sapiens
22 TMEM201 199953
Affinity Capture-MS Homo sapiens
23 SFXN2  
Affinity Capture-MS Homo sapiens
24 ERMP1 79956
Affinity Capture-MS Homo sapiens
25 GPX8 493869
Affinity Capture-MS Homo sapiens
26 ESYT2 57488
Affinity Capture-MS Homo sapiens
27 ATP13A3 79572
Affinity Capture-MS Homo sapiens
28 SLC12A7 10723
Affinity Capture-MS Homo sapiens
29 RDH10 157506
Affinity Capture-MS Homo sapiens
30 CTAGE5 4253
Affinity Capture-MS Homo sapiens
31 TMC6 11322
Affinity Capture-MS Homo sapiens
32 TMEM63B 55362
Affinity Capture-MS Homo sapiens
33 TMEM106C 79022
Affinity Capture-MS Homo sapiens
34 EXTL3 2137
Affinity Capture-MS Homo sapiens
35 NPC1 4864
Affinity Capture-MS Homo sapiens
36 SFXN3 81855
Affinity Capture-MS Homo sapiens
37 SCAP  
Affinity Capture-MS Homo sapiens
38 TUBA1A 7846
Affinity Capture-MS Homo sapiens
39 SREBF2 6721
Affinity Capture-MS Homo sapiens
40 ITPRIPL2 162073
Affinity Capture-MS Homo sapiens
41 C16orf58 64755
Affinity Capture-MS Homo sapiens
42 SYNJ2BP 55333
Affinity Capture-MS Homo sapiens
43 PIGT 51604
Affinity Capture-MS Homo sapiens
44 ALDH3B1 221
Affinity Capture-MS Homo sapiens
45 PIGS 94005
Affinity Capture-MS Homo sapiens
46 ITFG3 83986
Affinity Capture-MS Homo sapiens
47 RAD21 5885
Affinity Capture-MS Homo sapiens
48 TXNDC11 51061
Affinity Capture-MS Homo sapiens
49 LRRC8A 56262
Affinity Capture-MS Homo sapiens
50 SGPL1 8879
Affinity Capture-MS Homo sapiens
51 TMEM106B 54664
Affinity Capture-MS Homo sapiens
52 TMEM30A 55754
Affinity Capture-MS Homo sapiens
53 GPAA1 8733
Affinity Capture-MS Homo sapiens
54 SCD5  
Affinity Capture-MS Homo sapiens
55 TMEM39B  
Affinity Capture-MS Homo sapiens
56 SLC27A4 10999
Affinity Capture-MS Homo sapiens
57 FKBP8 23770
Affinity Capture-MS Homo sapiens
58 MYO19  
Affinity Capture-MS Homo sapiens
59 TMEM246 84302
Affinity Capture-MS Homo sapiens
60 HOXA1 3198
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 ABHD12 26090
Affinity Capture-MS Homo sapiens
62 LRRC8E 80131
Affinity Capture-MS Homo sapiens
63 Hoxa1  
Two-hybrid Mus musculus
64 EPHA7 2045
Affinity Capture-MS Homo sapiens
65 SMAD1 4086
Affinity Capture-MS Homo sapiens
66 YME1L1 10730
Affinity Capture-MS Homo sapiens
67 LMF1 64788
Affinity Capture-MS Homo sapiens
68 DHRS7B 25979
Affinity Capture-MS Homo sapiens
69 KCNN4 3783
Affinity Capture-MS Homo sapiens
70 LEMD3  
Affinity Capture-MS Homo sapiens
71 GGCX 2677
Affinity Capture-MS Homo sapiens
72 AGPAT2  
Affinity Capture-MS Homo sapiens
73 FZD6 8323
Affinity Capture-MS Homo sapiens
74 SUN1 23353
Affinity Capture-MS Homo sapiens
75 ABCB6 10058
Affinity Capture-MS Homo sapiens
76 SPRY2 10253
Two-hybrid Homo sapiens
77 SLCO4A1 28231
Affinity Capture-MS Homo sapiens
78 TNFSF14  
Affinity Capture-MS Homo sapiens
79 SLC25A40  
Affinity Capture-MS Homo sapiens
80 PIGK 10026
Affinity Capture-MS Homo sapiens
81 DDX58 23586
Affinity Capture-RNA Homo sapiens
82 EDEM3 80267
Affinity Capture-MS Homo sapiens
83 NUDT1  
Affinity Capture-MS Homo sapiens
84 FAM69A 388650
Affinity Capture-MS Homo sapiens
85 AGPAT6 137964
Affinity Capture-MS Homo sapiens
86 GLRB  
Affinity Capture-MS Homo sapiens
87 ALG11  
Affinity Capture-MS Homo sapiens
88 THEM6  
Affinity Capture-MS Homo sapiens
89 ENTPD4 9583
Affinity Capture-MS Homo sapiens
90 KIAA0922  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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