Gene description for ENTPD4
Gene name ectonucleoside triphosphate diphosphohydrolase 4
Gene symbol ENTPD4
Other names/aliases LALP70
LAP70
LYSAL1
NTPDase-4
UDPase
Species Homo sapiens
 Database cross references - ENTPD4
ExoCarta ExoCarta_9583
Vesiclepedia VP_9583
Entrez Gene 9583
HGNC 14573
MIM 607577
UniProt Q9Y227  
 ENTPD4 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for ENTPD4
Molecular Function
    GDP phosphatase activity GO:0004382 IBA
    GDP phosphatase activity GO:0004382 IDA
    protein binding GO:0005515 IPI
    nucleoside diphosphate phosphatase activity GO:0017110 IDA
    ribonucleoside triphosphate phosphatase activity GO:0017111 IBA
    ribonucleoside triphosphate phosphatase activity GO:0017111 IDA
    CDP phosphatase activity GO:0036384 IDA
    CTPase activity GO:0043273 IDA
    UDP phosphatase activity GO:0045134 IBA
    UDP phosphatase activity GO:0045134 IDA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    UDP catabolic process GO:0006256 IBA
    UDP catabolic process GO:0006256 IDA
    nucleobase-containing small molecule catabolic process GO:0034656 IDA
    CTP metabolic process GO:0046036 IBA
    CTP metabolic process GO:0046036 IDA
    GDP catabolic process GO:0046712 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    autophagosome membrane GO:0000421 IDA
    lysosomal membrane GO:0005765 IEA
    Golgi apparatus GO:0005794 IBA
    membrane GO:0016020 IBA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified ENTPD4 in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for ENTPD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRC55  
Affinity Capture-MS Homo sapiens
2 HFE  
Affinity Capture-MS Homo sapiens
3 PDZD8 118987
Affinity Capture-MS Homo sapiens
4 RPS27 6232
Two-hybrid Homo sapiens
5 TSPAN15 23555
Affinity Capture-MS Homo sapiens
6 ATP13A3 79572
Affinity Capture-MS Homo sapiens
7 TYW1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PCYOX1 51449
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 ATP2A1 487
Affinity Capture-MS Homo sapiens
11 GABRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GP9 2815
Affinity Capture-MS Homo sapiens
13 SCN3B  
Affinity Capture-MS Homo sapiens
14 GPR182  
Affinity Capture-MS Homo sapiens
15 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ATP2A3 489
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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