Gene description for TMC6
Gene name transmembrane channel-like 6
Gene symbol TMC6
Other names/aliases EV1
EVER1
EVIN1
LAK-4P
Species Homo sapiens
 Database cross references - TMC6
ExoCarta ExoCarta_11322
Vesiclepedia VP_11322
Entrez Gene 11322
HGNC 18021
MIM 605828
UniProt Q7Z403  
 TMC6 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for TMC6
Molecular Function
    protein binding GO:0005515 IPI
    mechanosensitive monoatomic ion channel activity GO:0008381 IBA
Biological Process
    intracellular zinc ion homeostasis GO:0006882 IDA
    monoatomic ion transmembrane transport GO:0034220 IEA
    protein stabilization GO:0050821 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
    nuclear membrane GO:0031965 IDA
    specific granule membrane GO:0035579 TAS
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified TMC6 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
4
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGCA  
Affinity Capture-MS Homo sapiens
2 ZBTB2 57621
Two-hybrid Homo sapiens
3 OR1D4  
Affinity Capture-MS Homo sapiens
4 TTYH1  
Affinity Capture-MS Homo sapiens
5 GP9 2815
Affinity Capture-MS Homo sapiens
6 ZDHHC12  
Affinity Capture-MS Homo sapiens
7 ZDHHC17 23390
Two-hybrid Homo sapiens
8 FASLG 356
Affinity Capture-MS Homo sapiens
9 TSPAN15 23555
Affinity Capture-MS Homo sapiens
10 B4GAT1 11041
Affinity Capture-MS Homo sapiens
11 CCR9  
Two-hybrid Homo sapiens
12 DDX58 23586
Affinity Capture-RNA Homo sapiens
13 KCNE3  
Affinity Capture-MS Homo sapiens
14 GP5 2814
Affinity Capture-MS Homo sapiens
15 C19orf26 255057
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 GPR182  
Affinity Capture-MS Homo sapiens
17 LRRC8E 80131
Affinity Capture-MS Homo sapiens
18 PIGH  
Affinity Capture-MS Homo sapiens
19 CLEC14A  
Affinity Capture-MS Homo sapiens
20 THEM6  
Affinity Capture-MS Homo sapiens
21 PXK 54899
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 C3orf52  
Affinity Capture-MS Homo sapiens
23 PCDHB3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMC6 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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