Gene description for FASLG
Gene name Fas ligand (TNF superfamily, member 6)
Gene symbol FASLG
Other names/aliases ALPS1B
APT1LG1
APTL
CD178
CD95-L
CD95L
FASL
TNFSF6
Species Homo sapiens
 Database cross references - FASLG
ExoCarta ExoCarta_356
Vesiclepedia VP_356
Entrez Gene 356
HGNC 11936
MIM 134638
UniProt P48023  
 FASLG identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 15940614    
Plasma 17076679    
T cells 21252909    
 Gene ontology annotations for FASLG
Molecular Function
    cytokine activity GO:0005125 IEA
    protein binding GO:0005515 IPI
    death receptor binding GO:0005123 IEA
    tumor necrosis factor receptor binding GO:0005164 IEA
    receptor binding GO:0005102 TAS
Biological Process
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    programmed cell death GO:0012501 TAS
    inflammatory cell apoptotic process GO:0006925 IEA
    cell-cell signaling GO:0007267 TAS
    positive regulation of endothelial cell apoptotic process GO:2000353 IDA
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    response to growth factor GO:0070848 IEA
    negative regulation of angiogenesis GO:0016525 IDA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IDA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    necroptotic signaling pathway GO:0097527 IDA
    cellular chloride ion homeostasis GO:0030644 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    regulation of necrotic cell death GO:0010939 TAS
    endosomal lumen acidification GO:0048388 IEA
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    response to lipopolysaccharide GO:0032496 IEA
    apoptotic signaling pathway GO:0097190 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    positive regulation of cell proliferation GO:0008284 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
    apoptotic process GO:0006915 TAS
    positive regulation of apoptotic process GO:0043065 IDA
    necroptotic process GO:0070266 TAS
    programmed necrotic cell death GO:0097300 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    T cell apoptotic process GO:0070231 IDA
    retinal cell programmed cell death GO:0046666 IEA
    immune response GO:0006955 IEA
    signal transduction GO:0007165 TAS
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IEA
Subcellular Localization
    integral component of plasma membrane GO:0005887 TAS
    caveola GO:0005901 IEA
    external side of plasma membrane GO:0009897 IEA
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 IDA
    extracellular space GO:0005615 IDA
    lysosomal lumen GO:0043202 IEA
    cytoplasmic membrane-bounded vesicle lumen GO:0060205 IEA
    extracellular region GO:0005576 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified FASLG in exosomes
1
Experiment ID 22
MISEV standards
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 15940614    
Organism Homo sapiens
Experiment description Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape.
Authors Huber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, Zaccheddu A, Colone M, Arancia G, Gentile M, Seregni E, Valenti R, Ballabio G, Belli F, Leo E, Parmiani G, Rivoltini L.
Journal name GASTRO
Publication year 2005
Sample Colorectal cancer cells
Sample name SW403
1869col
CRC28462
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 43
MISEV standards
EM
EV Biophysical techniques
CD83
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17076679    
Organism Homo sapiens
Experiment description Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences.
Authors Sabapatha A, Gercel-Taylor C, Taylor DD
Journal name AJRI
Publication year 2006
Sample Plasma
Sample name Plasma - Pregnant and not-pregnant women
Isolation/purification methods Size exclusion
Ultracentrifugation
Immunobeads (CD3, CD19, CD56, CD83, PLAP)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 91
MISEV standards
EM
EV Biophysical techniques
LAMP1|CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21252909    
Organism Homo sapiens
Experiment description Diacylglycerol kinase a regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes
Authors Alonso R, Mazzeo C, Rodriguez MC, Marsh M, Fraile-Ramos A, Calvo V, Avila-Flores A, Merida I, Izquierdo M.
Journal name CDD
Publication year 2011
Sample T cells
Sample name J-HM1-2.2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
FACS
Immunofluorescence
 Protein-protein interactions for FASLG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRAP2 9402
Invivo Homo sapiens
Invitro Homo sapiens
2 CASP8 841
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
3 FADD 8772
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
4 MMP7 4316
Biochemical Activity Homo sapiens
5 NCK1 4690
Invivo Homo sapiens
6 GRB2 2885
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 MSSE  
Invivo Homo sapiens
8 FAS 355
Invivo Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 EZR 7430
Invitro Homo sapiens
Invivo Homo sapiens
Co-purification Homo sapiens
10 PACSIN2 11252
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 LCK 3932
Invivo Homo sapiens
12 GRAP  
Invivo Homo sapiens
13 BID  
Co-purification Homo sapiens
14 TNFRSF6B  
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
15 APBB1  
Invivo Homo sapiens
16 PRPF40A 55660
Invivo Homo sapiens
17 NCF1  
Invivo Homo sapiens
18 FN1 2335
Reconstituted Complex Homo sapiens
19 ARHGDIA 396
Co-purification Homo sapiens
20 TNFRSF10B 8795
Co-purification Homo sapiens
21 FYN 2534
Invitro Homo sapiens
22 PTPN13 5783
Invitro Homo sapiens
23 CASP10  
Co-purification Homo sapiens
24 SRC 6714
Invivo Homo sapiens
25 FNBP1 23048
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
26 PIK3R1 5295
Invivo Homo sapiens
27 MSN 4478
Co-purification Homo sapiens
28 DMD  
Invivo Homo sapiens
29 RHOA 387
Co-purification Homo sapiens
30 MAPK8 5599
Co-purification Homo sapiens
31 TNFRSF1A 7132
Co-purification Homo sapiens
32 SUMO1 7341
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which FASLG is involved
PathwayEvidenceSource
FasL/ CD95L signaling TAS Reactome





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