Gene description for MYO15A
Gene name myosin XVA
Gene symbol MYO15A
Other names/aliases DFNB3
MYO15
Species Homo sapiens
 Database cross references - MYO15A
ExoCarta ExoCarta_51168
Vesiclepedia VP_51168
Entrez Gene 51168
HGNC 7594
MIM 602666
UniProt Q9UKN7  
 MYO15A identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for MYO15A
Molecular Function
    microfilament motor activity GO:0000146 IBA
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    endocytosis GO:0006897 IBA
    actin filament organization GO:0007015 IBA
    sensory perception of sound GO:0007605 IEA
    locomotory behavior GO:0007626 IEA
    response to light stimulus GO:0009416 IEA
    inner ear morphogenesis GO:0042472 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 IBA
    myosin complex GO:0016459 IEA
    stereocilium GO:0032420 IEA
    extracellular exosome GO:0070062 HDA
    actin-based cell projection GO:0098858 IBA
 Experiment description of studies that identified MYO15A in sEVs
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MYO15A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P4HB 5034
Cross-Linking-MS (XL-MS) Homo sapiens
2 JAZF1  
Affinity Capture-MS Homo sapiens
3 FASLG 356
Protein-peptide Homo sapiens
4 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
5 EPS8 2059
Two-hybrid Homo sapiens
6 HSF4  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
7 USP13  
Two-hybrid Homo sapiens
8 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



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