ExoCarta: Gene summary

Credits | Contact

Gene description for SRGAP1
Gene name	SLIT-ROBO Rho GTPase activating protein 1
Gene symbol	SRGAP1
Other names/aliases	ARHGAP13
Species	Homo sapiens
Database cross references - SRGAP1
ExoCarta	ExoCarta_57522
Vesiclepedia	VP_57522
Entrez Gene	57522
HGNC	17382
MIM	606523
UniProt	Q7Z6B7
SRGAP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Mesenchymal stem cells	36408942
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Prostate cancer cells	25844599

Gene ontology annotations for SRGAP1

Molecular Function
	GTPase activator activity	GO:0005096	TAS
	protein binding	GO:0005515	IPI
Biological Process
	signal transduction	GO:0007165	IEA
	negative regulation of cell migration	GO:0030336	IBA
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	cytosol	GO:0005829	TAS

Experiment description of studies that identified SRGAP1 in exosomes

Experiment ID

207

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DKO-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

208

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

Dks-8

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

209

MISEV standards
✘	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DLD-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

275

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|RAB5A\|CD9\|CD82\|CD63\|CD81	Enriched markers
✔ AIF	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25844599

Organism

Homo sapiens

Experiment description

Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.

Authors

"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."

Journal name

Oncotarget

Publication year

2015

Sample

Prostate cancer cells

Sample name

DU145 - Docetaxel sensitive

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.12-1.19 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry/Flow cytometry/Western blotting

Protein-protein interactions for SRGAP1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

YWHAH

7533

Affinity Capture-MS

Homo sapiens

RAC1

5879

FRET

Homo sapiens

ARHGAP1

392

Affinity Capture-MS

Homo sapiens

ARHGEF19

Affinity Capture-MS

Homo sapiens

WDR77

79084

Affinity Capture-MS

Homo sapiens

CDK8

1024

Affinity Capture-MS

Homo sapiens

YWHAZ

7534

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

YWHAB

7529

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

YWHAE

7531

Affinity Capture-MS

Homo sapiens

RHOA

387

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

FASLG

356

Protein-peptide

Homo sapiens

PTEN

5728

Affinity Capture-MS

Homo sapiens

ROBO1

6091

Affinity Capture-MS

Homo sapiens

SRGAP2

23380

Affinity Capture-MS

Homo sapiens

ELAVL1

1994

Affinity Capture-RNA

Homo sapiens

Actb

11461

Affinity Capture-MS

Mus musculus

PEG10

23089

Affinity Capture-MS

Homo sapiens

RAPH1

65059

Affinity Capture-MS

Homo sapiens

PRPS2

5634

Affinity Capture-MS

Homo sapiens

SNRNP70

6625

Affinity Capture-MS

Homo sapiens

PSPC1

55269

Affinity Capture-MS

Homo sapiens

SKA1

Affinity Capture-MS

Homo sapiens

RAD18

Affinity Capture-MS

Homo sapiens

KLHL22

84861

Affinity Capture-MS

Homo sapiens

FBXW7

Affinity Capture-MS

Homo sapiens

YWHAQ

10971

Affinity Capture-MS

Homo sapiens

SH3KBP1

30011

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PCM1

5108

Proximity Label-MS

Homo sapiens

YWHAG

7532

Affinity Capture-MS

Homo sapiens

ARHGEF10L

55160

Affinity Capture-MS

Homo sapiens

NKTR

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which SRGAP1 is involved

Pathway	Evidence	Source
Axon guidance	IEA	Reactome
CDC42 GTPase cycle	TAS	Reactome
Developmental Biology	IEA	Reactome
Inactivation of CDC42 and RAC1	IEA	Reactome
Nervous system development	IEA	Reactome
RAC1 GTPase cycle	TAS	Reactome
RHO GTPase cycle	TAS	Reactome
RHOA GTPase cycle	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome
Signaling by ROBO receptors	IEA	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here