Gene ontology annotations for YWHAQ
Experiment description of studies that identified YWHAQ in exosomes
1
Experiment ID
79
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
2
Experiment ID
80
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
3
Experiment ID
81
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
4
Experiment ID
76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
5
Experiment ID
20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
6
Experiment ID
21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
19837982
Organism
Homo sapiens
Experiment description
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors
Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name
MCP
Publication year
2009
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.10-1.12 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
7
Experiment ID
201
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22740476
Organism
Homo sapiens
Experiment description
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors
Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ.
Journal name
Electrophoresis
Publication year
2012
Sample
Colorectal cancer cells
Sample name
SW480
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
209
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
189
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19530224
Organism
Homo sapiens
Experiment description
Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors
Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name
J Cell Physiol
Publication year
2009
Sample
Keratinocytes
Sample name
Keratinocytes - Differentiated
Isolation/purification methods
Differential centrifugation Filtration Sucrose cushion Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
190
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19530224
Organism
Homo sapiens
Experiment description
Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors
Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name
J Cell Physiol
Publication year
2009
Sample
Keratinocytes
Sample name
Keratinocytes - Undifferentiated
Isolation/purification methods
Differential centrifugation Filtration Sucrose cushion Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
MNT-1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
G1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
15
Experiment ID
256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
501mel
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
16
Experiment ID
257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
Daju
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
17
Experiment ID
258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
18
Experiment ID
259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
19
Experiment ID
260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
20
Experiment ID
126
ISEV standards
✘
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
21
Experiment ID
219
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
C666-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
22
Experiment ID
220
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP69
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
23
Experiment ID
221
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP460
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
24
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
25
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
26
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
27
Experiment ID
231
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
232
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
29
Experiment ID
233
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
30
Experiment ID
138
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
31
Experiment ID
139
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
32
Experiment ID
140
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
VCaP - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
33
Experiment ID
275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
34
Experiment ID
274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
35
Experiment ID
66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
36
Experiment ID
191
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
37
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
38
Experiment ID
13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15326289
Organism
Homo sapiens
Experiment description
Identification and proteomic profiling of exosomes in human urine.
Authors
Pisitkun T, Shen RF, Knepper MA
Journal name
PNAS
Publication year
2004
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
39
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
40
Experiment ID
193
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
41
Experiment ID
194
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
42
Experiment ID
195
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
43
Experiment ID
196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
44
Experiment ID
197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for YWHAQ
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
LARP1
23367
Affinity Capture-MS
Homo sapiens
2
DDX6
1656
Affinity Capture-MS
Homo sapiens
3
KRT18
3875
Affinity Capture-Western
Homo sapiens
4
EPB41L2
2037
Affinity Capture-MS
Homo sapiens
5
DAPK1
Affinity Capture-Western
Homo sapiens
6
CRTC2
200186
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
7
EML3
256364
Affinity Capture-MS
Homo sapiens
8
ESR2
Reconstituted Complex
Homo sapiens
9
ARHGEF2
9181
Affinity Capture-Western
Homo sapiens
10
KLC2
64837
Affinity Capture-MS
Homo sapiens
11
PIK3CA
5290
Reconstituted Complex
Homo sapiens
12
BAX
581
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
13
NIF3L1
60491
Affinity Capture-Western
Homo sapiens
14
RASGRF1
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
15
BAD
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
16
MARK3
4140
Affinity Capture-MS
Homo sapiens
17
TSC2
7249
Affinity Capture-MS
Homo sapiens
18
CDKN1B
1027
Affinity Capture-Western
Homo sapiens
19
SUV420H1
Affinity Capture-MS
Homo sapiens
20
PANK2
80025
Affinity Capture-MS
Homo sapiens
21
SH3BP4
23677
Affinity Capture-MS
Homo sapiens
22
SRSF6
6431
Affinity Capture-MS
Homo sapiens
23
KIF23
9493
Affinity Capture-MS
Homo sapiens
24
EPB41L3
23136
Affinity Capture-MS
Homo sapiens
25
SRSF1
6426
Affinity Capture-MS
Homo sapiens
26
RPL21
6144
Affinity Capture-MS
Homo sapiens
27
SPIRE1
56907
Affinity Capture-MS
Homo sapiens
28
NCOA1
Reconstituted Complex
Homo sapiens
29
IRS2
8660
Affinity Capture-MS
Homo sapiens
30
UCP2
Invitro
Homo sapiens
31
CDC25B
994
Invitro
Homo sapiens
Invivo
Homo sapiens
32
CBWD2
Affinity Capture-MS
Homo sapiens
33
PRKD1
5587
Invivo
Homo sapiens
Invitro
Homo sapiens
Reconstituted Complex
Homo sapiens
34
RABEP1
Affinity Capture-MS
Homo sapiens
35
PDPK1
Invivo
Homo sapiens
Invitro
Homo sapiens
36
PNKP
11284
Affinity Capture-MS
Homo sapiens
37
MEF2D
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
38
KIF5C
3800
Affinity Capture-MS
Homo sapiens
39
FAM83G
Affinity Capture-MS
Homo sapiens
40
SRSF3
6428
Affinity Capture-MS
Homo sapiens
41
KLC4
89953
Affinity Capture-MS
Homo sapiens
42
Wbscr14
Two-hybrid
Mus musculus
Affinity Capture-Western
Mus musculus
43
CBL
867
Invivo
Homo sapiens
Invitro
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
44
RAI14
26064
Affinity Capture-MS
Homo sapiens
45
THRA
Reconstituted Complex
Homo sapiens
46
M-RIP
Affinity Capture-MS
Homo sapiens
47
MARK2
2011
Affinity Capture-MS
Homo sapiens
48
TJP2
9414
Affinity Capture-MS
Homo sapiens
49
REM1
Far Western
Homo sapiens
50
MLLT4
4301
Affinity Capture-MS
Homo sapiens
51
UCP3
Invitro
Homo sapiens
52
FXYD1
Reconstituted Complex
Homo sapiens
53
PAK4
10298
Affinity Capture-MS
Homo sapiens
54
PFKFB2
5208
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
55
EDC3
80153
Affinity Capture-MS
Homo sapiens
56
C22orf9
Affinity Capture-MS
Homo sapiens
57
ESR1
Reconstituted Complex
Homo sapiens
58
KIF5B
3799
Affinity Capture-MS
Homo sapiens
59
PI4KB
5298
Affinity Capture-MS
Homo sapiens
60
RASGRP3
Affinity Capture-MS
Homo sapiens
61
PPFIBP1
8496
Affinity Capture-MS
Homo sapiens
62
NRIP1
8204
Co-localization
Homo sapiens
Reconstituted Complex
Homo sapiens
Phenotypic Suppression
Homo sapiens
63
RAF1
5894
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Far Western
Homo sapiens
Reconstituted Complex
Homo sapiens
64
SRSF10
10772
Affinity Capture-MS
Homo sapiens
65
KIAA1409
Affinity Capture-MS
Homo sapiens
66
PIK3CG
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
67
BRAF
Affinity Capture-MS
Homo sapiens
68
USP8
Affinity Capture-MS
Homo sapiens
69
CCAR1
55749
Affinity Capture-Western
Homo sapiens
70
LYST
1130
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
71
NCOA3
Reconstituted Complex
Homo sapiens
72
FLJ10211
Affinity Capture-MS
Homo sapiens
73
CALML5
51806
Affinity Capture-MS
Homo sapiens
74
NFATC2
Invitro
Homo sapiens
75
HDAC5
10014
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
76
YAP1
Affinity Capture-MS
Homo sapiens
77
LUC7L2
Affinity Capture-MS
Homo sapiens
78
PPARBP
Reconstituted Complex
Homo sapiens
79
PAK6
56924
Affinity Capture-MS
Homo sapiens
80
NFATC4
4776
Invitro
Homo sapiens
81
AR
367
Reconstituted Complex
Homo sapiens
82
DENND4A
10260
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which YWHAQ is involved