Gene description for LYST
Gene name lysosomal trafficking regulator
Gene symbol LYST
Other names/aliases CHS
CHS1
Species Homo sapiens
 Database cross references - LYST
ExoCarta ExoCarta_1130
Entrez Gene 1130
HGNC 1968
MIM 606897
UniProt Q99698  
 LYST identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for LYST
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
Biological Process
    endosome to lysosome transport via multivesicular body sorting pathway GO:0032510 IMP
    pigmentation GO:0043473 IMP
    microtubule-based process GO:0007017 IEA
    neutrophil mediated immunity GO:0002446 IEA
    melanosome organization GO:0032438 ISS
    blood coagulation GO:0007596 IEA
    lysosome organization GO:0007040 IEA
    natural killer cell mediated cytotoxicity GO:0042267 IMP
    response to drug GO:0042493 IEA
    leukocyte chemotaxis GO:0030595 ISS
    phospholipid metabolic process GO:0006644 IEA
    positive regulation of natural killer cell activation GO:0032816 IEA
    phospholipid homeostasis GO:0055091 IEA
    defense response to virus GO:0051607 ISS
    secretion of lysosomal enzymes GO:0033299 IEA
    mast cell secretory granule organization GO:0033364 ISS
    defense response to protozoan GO:0042832 ISS
    defense response to bacterium GO:0042742 ISS
    T cell mediated immunity GO:0002456 IEA
Subcellular Localization
    cytosol GO:0005829 IEA
    endomembrane system GO:0012505 IBA
    microtubule cytoskeleton GO:0015630 IDA
    extrinsic component of membrane GO:0019898 IBA
 Experiment description of studies that identified LYST in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for LYST
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LIP8  
Two-hybrid Homo sapiens
2 DNAJC14  
Invitro Homo sapiens
3 YWHAQ 10971
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 HGS 9146
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 KPNB1 3837
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 ATN1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
7 DGCR14  
Invitro Homo sapiens
Two-hybrid Homo sapiens
8 MED12  
Invivo Homo sapiens
Two-hybrid Homo sapiens
9 CSNK2B 1460
Invitro Homo sapiens
Two-hybrid Homo sapiens
10 CSNK2A1 1457
Two-hybrid Homo sapiens
11 HNRNPM 4670
Invivo Homo sapiens
Two-hybrid Homo sapiens
12 ESRRA  
Invivo Homo sapiens
Two-hybrid Homo sapiens
13 NCDN  
Invivo Homo sapiens
Two-hybrid Homo sapiens
14 MRPL17  
Two-hybrid Homo sapiens
15 YWHAB 7529
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 CALM1 801
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which LYST is involved
No pathways found





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