ExoCarta: Gene summary

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Gene description for GAR1
Gene name	GAR1 ribonucleoprotein
Gene symbol	GAR1
Other names/aliases	NOLA1
Species	Homo sapiens
Database cross references - GAR1
ExoCarta	ExoCarta_54433
Vesiclepedia	VP_54433
Entrez Gene	54433
HGNC	14264
MIM	606468
UniProt	Q9NY12
GAR1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513

Gene ontology annotations for GAR1

Molecular Function
	RNA binding	GO:0003723	HDA
	RNA binding	GO:0003723	IPI
	protein binding	GO:0005515	IPI
	box H/ACA snoRNA binding	GO:0034513	IBA
	box H/ACA snoRNA binding	GO:0034513	IPI
	telomerase RNA binding	GO:0070034	IPI
Biological Process
	snoRNA guided rRNA pseudouridine synthesis	GO:0000454	IBA
	telomere maintenance via telomerase	GO:0007004	IDA
	telomere maintenance via telomerase	GO:0007004	NAS
	telomere maintenance via telomerase	GO:0007004	TAS
Subcellular Localization
	chromosome, telomeric region	GO:0000781	HDA
	chromosome, telomeric region	GO:0000781	IDA
	fibrillar center	GO:0001650	IDA
	nucleoplasm	GO:0005654	IDA
	nucleoplasm	GO:0005654	TAS
	telomerase holoenzyme complex	GO:0005697	IDA
	telomerase holoenzyme complex	GO:0005697	NAS
	telomerase holoenzyme complex	GO:0005697	TAS
	box H/ACA snoRNP complex	GO:0031429	IBA
	box H/ACA snoRNP complex	GO:0031429	IDA
	box H/ACA snoRNP complex	GO:0031429	TAS
	box H/ACA scaRNP complex	GO:0072589	TAS
	box H/ACA telomerase RNP complex	GO:0090661	IDA
	box H/ACA telomerase RNP complex	GO:0090661	TAS

Experiment description of studies that identified GAR1 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

207

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DKO-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

208

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

Dks-8

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

209

MISEV standards
✘	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DLD-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for GAR1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

PCBP2

5094

Two-hybrid

Homo sapiens

KIF16B

55614

Affinity Capture-MS

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

MRPS27

23107

Affinity Capture-MS

Homo sapiens

RPSAP58

388524

Affinity Capture-MS

Homo sapiens

UBE2H

7328

Affinity Capture-MS

Homo sapiens

RHOF

54509

Affinity Capture-MS

Homo sapiens

RIOK1

83732

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZNF346

Affinity Capture-MS

Homo sapiens

DOCK4

9732

Affinity Capture-MS

Homo sapiens

RPS4X

6191

Co-fractionation

Homo sapiens

MRPL17

Affinity Capture-MS

Homo sapiens

TFB2M

Affinity Capture-MS

Homo sapiens

TNPO1

3842

Co-fractionation

Homo sapiens

ANGEL2

90806

Affinity Capture-MS

Homo sapiens

FGD1

Affinity Capture-MS

Homo sapiens

Skp1a

21402

Affinity Capture-MS

Mus musculus

RPL37A

6168

Affinity Capture-MS

Homo sapiens

ALB

213

Affinity Capture-MS

Homo sapiens

TSNAX

7257

Affinity Capture-MS

Homo sapiens

BRCA1

672

Affinity Capture-MS

Homo sapiens

ESF1

51575

Affinity Capture-MS

Homo sapiens

KIF20A

10112

Affinity Capture-MS

Homo sapiens

ZNF71

Affinity Capture-MS

Homo sapiens

MRPL50

54534

Affinity Capture-MS

Homo sapiens

SAP30

8819

Affinity Capture-MS

Homo sapiens

TAF1

Affinity Capture-MS

Homo sapiens

IGHG2

3501

Affinity Capture-MS

Homo sapiens

USP36

Affinity Capture-Western

Homo sapiens

GPATCH4

54865

Affinity Capture-MS

Homo sapiens

PLEKHG4

Affinity Capture-MS

Homo sapiens

SNRNP70

6625

Affinity Capture-MS

Homo sapiens

NPAT

Affinity Capture-MS

Homo sapiens

KLF8

Affinity Capture-MS

Homo sapiens

USP39

10713

Affinity Capture-MS

Homo sapiens

Htatsf1

Two-hybrid

Mus musculus

HIST2H3A

333932

Cross-Linking-MS (XL-MS)

Homo sapiens

FBXW7

Affinity Capture-MS

Homo sapiens

PRC1

9055

Affinity Capture-MS

Homo sapiens

ACPP

Affinity Capture-MS

Homo sapiens

FBL

2091

Proximity Label-MS

Homo sapiens

OBSL1

23363

Affinity Capture-MS

Homo sapiens

Jup

16480

Affinity Capture-MS

Mus musculus

SRP14

6727

Co-fractionation

Homo sapiens

Ppp4c

56420

Affinity Capture-MS

Mus musculus

PRKRA

8575

Affinity Capture-MS

Homo sapiens

FANCD2

Affinity Capture-MS

Homo sapiens

EXOSC4

54512

Affinity Capture-MS

Homo sapiens

Nhp2l1

20826

Affinity Capture-MS

Mus musculus

DDX18

8886

Co-fractionation

Homo sapiens

KLHL20

Affinity Capture-MS

Homo sapiens

NHP2

55651

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

Mcm3

17215

Affinity Capture-MS

Mus musculus

ARHGAP22

58504

Affinity Capture-MS

Homo sapiens

MAK16

Affinity Capture-MS

Homo sapiens

RPL19

6143

Affinity Capture-MS

Homo sapiens

GEMIN2

8487

Affinity Capture-Western

Homo sapiens

KIF14

9928

Affinity Capture-MS

Homo sapiens

FOXP1

27086

Protein-RNA

Homo sapiens

ZCRB1

Affinity Capture-MS

Homo sapiens

CHMP4C

92421

Affinity Capture-MS

Homo sapiens

GEMIN5

25929

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPS25

64432

Affinity Capture-MS

Homo sapiens

POLD1

5424

Affinity Capture-MS

Homo sapiens

FOXA1

Affinity Capture-MS

Homo sapiens

HIST1H4A

8359

Affinity Capture-MS

Homo sapiens

USP10

9100

Affinity Capture-MS

Homo sapiens

RPL4

6124

Affinity Capture-MS

Homo sapiens

RPS10

6204

Affinity Capture-MS

Homo sapiens

C17orf49

124944

Affinity Capture-MS

Homo sapiens

TRA2A

29896

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZMYM1

79830

Affinity Capture-MS

Homo sapiens

SRSF6

6431

Affinity Capture-MS

Homo sapiens

HIST1H2AM

8336

Affinity Capture-MS

Homo sapiens

DDX20

11218

Affinity Capture-Western

Homo sapiens

UBR5

51366

Affinity Capture-MS

Homo sapiens

RAP1B

5908

Affinity Capture-MS

Homo sapiens

C2orf44

Affinity Capture-MS

Homo sapiens

GSTA1

2938

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BTF3

689

Affinity Capture-MS

Homo sapiens

MAPT

Affinity Capture-MS

Homo sapiens

DDX51

Affinity Capture-MS

Homo sapiens

ASTE1

Affinity Capture-MS

Homo sapiens

HIST1H1E

3008

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FARP1

10160

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SYNCRIP

10492

Affinity Capture-MS

Homo sapiens

RRP36

88745

Affinity Capture-MS

Homo sapiens

CBX6

Affinity Capture-MS

Homo sapiens

TRIM11

Affinity Capture-MS

Homo sapiens

COPRS

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LYAR

55646

Affinity Capture-MS

Homo sapiens

HECTD1

25831

Affinity Capture-MS

Homo sapiens

CTR9

9646

Affinity Capture-MS

Homo sapiens

LYST

1130

Affinity Capture-MS

Homo sapiens

SRPK3

Affinity Capture-MS

Homo sapiens

Dctn3

Affinity Capture-MS

Mus musculus

DAXX

Affinity Capture-MS

Homo sapiens

RPL11

6135

Affinity Capture-MS

Homo sapiens

100

WDR46

Affinity Capture-MS

Homo sapiens

101

ZBTB2

57621

Affinity Capture-MS

Homo sapiens

102

RPS19

6223

Affinity Capture-MS

Homo sapiens

103

MRPL21

219927

Affinity Capture-MS

Homo sapiens

104

HIST1H3A

8350

Cross-Linking-MS (XL-MS)

Homo sapiens

105

PREX1

Affinity Capture-MS

Homo sapiens

106

ABT1

29777

Affinity Capture-MS

Homo sapiens

107

ARHGAP11B

Affinity Capture-MS

Homo sapiens

108

CHN2

Affinity Capture-MS

Homo sapiens

109

RPL35

11224

Affinity Capture-MS

Homo sapiens

110

C1orf86

Affinity Capture-MS

Homo sapiens

111

RASGRF2

Affinity Capture-MS

Homo sapiens

112

PRMT8

Affinity Capture-MS

Homo sapiens

113

NSD1

Affinity Capture-MS

Homo sapiens

114

SHQ1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

115

NR3C1

2908

Proximity Label-MS

Homo sapiens

116

RPL3

6122

Affinity Capture-MS

Homo sapiens

117

TTLL5

Affinity Capture-MS

Homo sapiens

118

ARHGEF16

27237

Affinity Capture-MS

Homo sapiens

119

FN1

2335

Affinity Capture-MS

Homo sapiens

120

ZC3H10

Affinity Capture-MS

Homo sapiens

121

Vps4b

20479

Affinity Capture-MS

Mus musculus

122

CAND1

55832

Affinity Capture-MS

Homo sapiens

123

MYCN

Affinity Capture-MS

Homo sapiens

124

GEMIN4

50628

Affinity Capture-Western

Homo sapiens

125

FARP2

9855

Affinity Capture-MS

Homo sapiens

126

RBM4B

Affinity Capture-MS

Homo sapiens

127

DKC1

1736

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

128

MYC

Affinity Capture-MS

Homo sapiens

129

DLST

1743

Affinity Capture-MS

Homo sapiens

130

DDRGK1

65992

Affinity Capture-MS

Homo sapiens

131

RNF2

Affinity Capture-MS

Homo sapiens

132

Nedd1

Affinity Capture-MS

Mus musculus

133

TSR1

55720

Co-fractionation

Homo sapiens

134

ZC3H3

Affinity Capture-MS

Homo sapiens

135

UBXN1

51035

Affinity Capture-MS

Homo sapiens

136

DOCK3

Affinity Capture-MS

Homo sapiens

137

SNRPD2

6633

Affinity Capture-MS

Homo sapiens

138

RPL13

6137

Affinity Capture-MS

Homo sapiens

139

CEP89

84902

Affinity Capture-MS

Homo sapiens

140

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

141

PUS7

54517

Co-fractionation

Homo sapiens

142

Arhgap33

Affinity Capture-MS

Mus musculus

143

SMN1

6606

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens

144

Sod1

20655

Affinity Capture-MS

Mus musculus

145

WDR77

79084

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

146

Osgep

Affinity Capture-MS

Mus musculus

147

MNDA

4332

Affinity Capture-MS

Homo sapiens

148

ISCA2

122961

Affinity Capture-MS

Homo sapiens

149

POLR1C

9533

Co-fractionation

Homo sapiens

150

KEAP1

9817

Affinity Capture-MS

Homo sapiens

151

WRAP53

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

152

CLNS1A

1207

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

153

TERC

Affinity Capture-Western

Homo sapiens

154

PTPN2

5771

Affinity Capture-MS

Homo sapiens

155

CSPP1

Affinity Capture-MS

Homo sapiens

156

PRMT5

10419

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

157

PRDM5

Affinity Capture-MS

Homo sapiens

158

UFL1

23376

Affinity Capture-MS

Homo sapiens

159

KRR1

11103

Affinity Capture-MS

Homo sapiens

160

NLN

57486

Affinity Capture-MS

Homo sapiens

161

NEIL1

Affinity Capture-MS

Homo sapiens

162

OTUD4

Affinity Capture-MS

Homo sapiens

163

IL17B

27190

Affinity Capture-MS

Homo sapiens

164

ARHGAP36

Affinity Capture-MS

Homo sapiens

165

RPSA

3921

Affinity Capture-MS

Homo sapiens

166

STX3

6809

Affinity Capture-MS

Homo sapiens

167

RPL7A

6130

Affinity Capture-MS

Homo sapiens

168

HIST1H2AB

8335

Cross-Linking-MS (XL-MS)

Homo sapiens

169

RPL27

6155

Affinity Capture-MS

Homo sapiens

170

CUL7

9820

Affinity Capture-MS

Homo sapiens

171

Ufl1

Affinity Capture-MS

Mus musculus

172

HSPG2

3339

Affinity Capture-MS

Homo sapiens

173

RPL36AL

6166

Affinity Capture-MS

Homo sapiens

174

PTCH1

Affinity Capture-MS

Homo sapiens

175

PRMT1

3276

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

176

NOP10

55505

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

177

LRRC1

55227

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which GAR1 is involved

Pathway	Evidence	Source
Cell Cycle	TAS	Reactome
Chromosome Maintenance	TAS	Reactome
Extension of Telomeres	TAS	Reactome
Metabolism of RNA	TAS	Reactome
rRNA modification in the nucleus and cytosol	TAS	Reactome
rRNA processing	TAS	Reactome
rRNA processing in the nucleus and cytosol	TAS	Reactome
Telomere Extension By Telomerase	TAS	Reactome
Telomere Maintenance	TAS	Reactome

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