Gene description for Nhp2l1
Gene name NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
Gene symbol Nhp2l1
Other names/aliases FA-1
Fta1
Ssfa1
Species Mus musculus
 Database cross references - Nhp2l1
ExoCarta ExoCarta_20826
Vesiclepedia VP_20826
Entrez Gene 20826
UniProt Q9D0T1  
 Nhp2l1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Nhp2l1
Molecular Function
    RNA binding GO:0003723 IBA
    snoRNA binding GO:0030515 ISO
    U4atac snRNA binding GO:0030622 ISO
    U4atac snRNA binding GO:0030622 ISS
    box C/D sno(s)RNA binding GO:0034512 IMP
    ATPase binding GO:0051117 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    box C/D snoRNP assembly GO:0000492 IMP
    single fertilization GO:0007338 IDA
    maturation of SSU-rRNA GO:0030490 IBA
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    U4atac snRNP GO:0005690 ISO
    U4atac snRNP GO:0005690 ISS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IEA
    plasma membrane GO:0005886 ISS
    box C/D methylation guide snoRNP complex GO:0031428 IBA
    box C/D methylation guide snoRNP complex GO:0031428 IMP
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    protein-containing complex GO:0032991 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISS
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    precatalytic spliceosome GO:0071011 IBA
 Experiment description of studies that identified Nhp2l1 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Nhp2l1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RSL1D1 26156
Affinity Capture-MS Homo sapiens
2 Eed  
Affinity Capture-MS Mus musculus
3 DHX15 1665
Affinity Capture-MS Homo sapiens
4 NHP2 55651
Affinity Capture-MS Homo sapiens
5 EXOSC7 23016
Two-hybrid Homo sapiens
6 ZNHIT6 54680
Affinity Capture-MS Homo sapiens
7 NOP58 51602
Affinity Capture-MS Homo sapiens
8 IPO5 3843
Affinity Capture-MS Homo sapiens
9 SNRPE 6635
Affinity Capture-MS Homo sapiens
10 PABPC4 8761
Affinity Capture-MS Homo sapiens
11 KPNA1 3836
Affinity Capture-MS Homo sapiens
12 GAR1 54433
Affinity Capture-MS Homo sapiens
13 RUVBL2 10856
Affinity Capture-MS Homo sapiens
14 Tfe3  
Affinity Capture-MS Mus musculus
15 SNRPB 6628
Affinity Capture-MS Homo sapiens
16 TGS1  
Affinity Capture-MS Homo sapiens
17 KPNB1 3837
Affinity Capture-MS Homo sapiens
18 NOP56 10528
Affinity Capture-MS Homo sapiens
19 KPNA6 23633
Affinity Capture-MS Homo sapiens
20 WRAP53  
Affinity Capture-MS Homo sapiens
21 DDX21 9188
Affinity Capture-MS Homo sapiens
22 URB1 9875
Affinity Capture-MS Homo sapiens
23 SSB 6741
Affinity Capture-MS Homo sapiens
24 KPNA2 3838
Affinity Capture-MS Homo sapiens
25 DHX30 22907
Affinity Capture-MS Homo sapiens
26 HNRNPF 3185
Affinity Capture-MS Homo sapiens
27 RRP9 9136
Affinity Capture-MS Homo sapiens
28 SNRPD2 6633
Affinity Capture-MS Homo sapiens
29 TARDBP 23435
Affinity Capture-MS Homo sapiens
30 LARP7 51574
Affinity Capture-MS Homo sapiens
31 GPATCH4 54865
Affinity Capture-MS Homo sapiens
32 C1QBP 708
Affinity Capture-MS Homo sapiens
33 PABPC1 26986
Affinity Capture-MS Homo sapiens
34 MOV10 4343
Affinity Capture-MS Homo sapiens
35 SNRPD1 6632
Affinity Capture-MS Homo sapiens
36 NOLC1 9221
Affinity Capture-MS Homo sapiens
37 NHP2L1 4809
Affinity Capture-MS Homo sapiens
38 NUFIP1  
Affinity Capture-MS Homo sapiens
39 DKC1 1736
Affinity Capture-MS Homo sapiens
40 SART3 9733
Affinity Capture-MS Homo sapiens
41 ZNHIT3  
Affinity Capture-MS Homo sapiens
42 WDR43 23160
Affinity Capture-MS Homo sapiens
43 KRT31 3881
Two-hybrid Homo sapiens
44 HNRNPUL1 11100
Affinity Capture-MS Homo sapiens
45 DHX9 1660
Affinity Capture-MS Homo sapiens
46 FBL 2091
Affinity Capture-MS Homo sapiens
47 RUVBL1 8607
Affinity Capture-MS Homo sapiens
48 NOP10 55505
Affinity Capture-MS Homo sapiens
49 COIL  
Affinity Capture-MS Homo sapiens
50 Fancd2  
Affinity Capture-MS Mus musculus
51 SNRPD3 6634
Affinity Capture-MS Homo sapiens
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