Gene description for KPNA1
Gene name karyopherin alpha 1 (importin alpha 5)
Gene symbol KPNA1
Other names/aliases IPOA5
NPI-1
RCH2
SRP1
Species Homo sapiens
 Database cross references - KPNA1
ExoCarta ExoCarta_3836
Entrez Gene 3836
HGNC 6394
MIM 600686
UniProt P52294  
 KPNA1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for KPNA1
Molecular Function
    nuclear localization sequence binding GO:0008139 TAS
    protein transporter activity GO:0008565 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of DNA recombination GO:0000018 TAS
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    intracellular transport of virus GO:0075733 TAS
    viral life cycle GO:0019058 TAS
    positive regulation of protein import into nucleus GO:0042307 ISS
    cytokine-mediated signaling pathway GO:0019221 TAS
    programmed cell death GO:0012501 TAS
    viral process GO:0016032 TAS
    NLS-bearing protein import into nucleus GO:0006607 TAS
    apoptotic DNA fragmentation GO:0006309 TAS
    apoptotic process GO:0006915 TAS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    nuclear pore GO:0005643 TAS
    cytosol GO:0005829 TAS
    dendrite GO:0030425 ISS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified KPNA1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KPNA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STAT1 6772
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 NUP54  
Reconstituted Complex Homo sapiens
3 NUP62 23636
Reconstituted Complex Homo sapiens
4 SRP19  
Affinity Capture-MS Homo sapiens
5 LYAR 55646
Affinity Capture-MS Homo sapiens
6 PAX5  
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 RUNX1T1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 KPNB1 3837
Reconstituted Complex Homo sapiens
9 RAG1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 RAG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 CSE1L 1434
Reconstituted Complex Homo sapiens
12 EDD  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 RANBP2 5903
Reconstituted Complex Homo sapiens
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