Gene description for TRMT61A
Gene name tRNA methyltransferase 61A
Gene symbol TRMT61A
Other names/aliases C14orf172
GCD14
Gcd14p
TRM61
hTRM61
Species Homo sapiens
 Database cross references - TRMT61A
ExoCarta ExoCarta_115708
Vesiclepedia VP_115708
Entrez Gene 115708
HGNC 23790
UniProt Q96FX7  
 TRMT61A identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TRMT61A
Molecular Function
    protein binding GO:0005515 IPI
    mRNA (adenine-N1-)-methyltransferase activity GO:0061953 IDA
    tRNA (adenine(58)-N1)-methyltransferase activity GO:0160107 IEA
Biological Process
    mRNA processing GO:0006397 IDA
    tRNA methylation GO:0030488 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    tRNA (m1A) methyltransferase complex GO:0031515 IBA
    tRNA (m1A) methyltransferase complex GO:0031515 IPI
 Experiment description of studies that identified TRMT61A in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRMT61A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 N4BP2L2 10443
Affinity Capture-MS Homo sapiens
2 VPS36 51028
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 VPS35 55737
Co-fractionation Homo sapiens
5 CCT7 10574
Affinity Capture-MS Homo sapiens
6 PBLD 64081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NMT1 4836
Co-fractionation Homo sapiens
8 GTF3C2  
Affinity Capture-MS Homo sapiens
9 SIRT6  
Affinity Capture-MS Homo sapiens
10 KPNA1 3836
Co-fractionation Homo sapiens
11 KRT18 3875
Co-fractionation Homo sapiens
12 ARHGEF11 9826
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 RICTOR 253260
Affinity Capture-MS Homo sapiens
14 CDC42BPG 55561
Affinity Capture-MS Homo sapiens
15 SSB 6741
Affinity Capture-MS Homo sapiens
16 CCT6A 908
Affinity Capture-MS Homo sapiens
17 KLHL26  
Affinity Capture-MS Homo sapiens
18 SRP9 6726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 LIMCH1 22998
Co-fractionation Homo sapiens
20 TUBB8 347688
Affinity Capture-MS Homo sapiens
21 WRAP53  
Affinity Capture-MS Homo sapiens
22 TCL1A  
Two-hybrid Homo sapiens
23 TCF7L2  
Affinity Capture-MS Homo sapiens
24 CCP110  
Affinity Capture-MS Homo sapiens
25 EGFR 1956
Negative Genetic Homo sapiens
26 TRMT6 51605
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CCT6B 10693
Affinity Capture-MS Homo sapiens
28 CAND1 55832
Affinity Capture-MS Homo sapiens
29 WDR59 79726
Affinity Capture-MS Homo sapiens
30 SBF1 6305
Affinity Capture-MS Homo sapiens
31 EVL 51466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MARS 4141
Co-fractionation Homo sapiens
33 EIF2A 83939
Affinity Capture-MS Homo sapiens
34 HEXA 3073
Co-fractionation Homo sapiens
35 G6PD 2539
Co-fractionation Homo sapiens
36 INPP4B 8821
Co-fractionation Homo sapiens
37 RBFOX2 23543
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TLN2 83660
Co-fractionation Homo sapiens
39 EMILIN1 11117
Affinity Capture-MS Homo sapiens
40 MYDGF 56005
Cross-Linking-MS (XL-MS) Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 TUBA1A 7846
Affinity Capture-MS Homo sapiens
43 HELZ 9931
Affinity Capture-MS Homo sapiens
44 MTOR 2475
Co-fractionation Homo sapiens
45 IFI16 3428
Affinity Capture-MS Homo sapiens
46 SSSCA1 10534
Affinity Capture-MS Homo sapiens
47 CCT2 10576
Affinity Capture-MS Homo sapiens
48 RLIM 51132
Affinity Capture-MS Homo sapiens
49 AP1B1 162
Co-fractionation Homo sapiens
50 COPS5 10987
Affinity Capture-MS Homo sapiens
51 SKIV2L2 23517
Co-fractionation Homo sapiens
52 NPM1 4869
Co-fractionation Homo sapiens
53 CAMK2D 817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ARHGEF12 23365
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 AKT1 207
Co-fractionation Homo sapiens
56 NANOG  
Affinity Capture-MS Homo sapiens
57 FHL2 2274
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 METTL1 4234
Co-fractionation Homo sapiens
59 SOX2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TRMT61A is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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