Experiment description of studies that identified CDC42BPG in exosomes

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for CDC42BPG

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAH 7533 Affinity Capture-MS Homo sapiens 2 ATG16L1 55054 Affinity Capture-MS Homo sapiens 3 TRMT61A 115708 Affinity Capture-MS Homo sapiens 4 FBXW5   Affinity Capture-MS Homo sapiens 5 RHOQ 23433 Reconstituted Complex Homo sapiens 6 FAM167A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 HSPA8 3312 Affinity Capture-MS Homo sapiens 8 YWHAZ 7534 Affinity Capture-MS Homo sapiens 9 POU1F1   Affinity Capture-MS Homo sapiens 10 METTL21B   Affinity Capture-MS Homo sapiens 11 CDC42 998 Reconstituted Complex Homo sapiens 12 TBK1 29110 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 13 CRYBB3   Affinity Capture-MS Homo sapiens 14 SSSCA1 10534 Affinity Capture-MS Homo sapiens 15 KXD1 79036 Affinity Capture-MS Homo sapiens 16 RPL28 6158 Affinity Capture-MS Homo sapiens 17 AR 367 Affinity Capture-MS Homo sapiens 18 RAC1 5879 Reconstituted Complex Homo sapiens 19 DYNLL2 140735 Affinity Capture-MS Homo sapiens 20 LURAP1L   Affinity Capture-MS Homo sapiens 21 LURAP1   Affinity Capture-MS Homo sapiens 22 FOXD4L6   Affinity Capture-MS Homo sapiens 23 DYNLL1 8655 Affinity Capture-MS Homo sapiens 24 UBXN6 80700 Affinity Capture-MS Homo sapiens View the network

Pathways in which CDC42BPG is involved

No pathways found