Gene description for DYNLL2
Gene name dynein, light chain, LC8-type 2
Gene symbol DYNLL2
Other names/aliases DNCL1B
Dlc2
RSPH22
Species Homo sapiens
 Database cross references - DYNLL2
ExoCarta ExoCarta_140735
Vesiclepedia VP_140735
Entrez Gene 140735
HGNC 24596
MIM 608942
UniProt Q96FJ2  
 DYNLL2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DYNLL2
Molecular Function
    cytoskeletal protein binding GO:0008092 IEA
    protein binding GO:0005515 IPI
    motor activity GO:0003774 IEA
Biological Process
    transport GO:0006810 IEA
    microtubule-based process GO:0007017 IEA
    metabolic process GO:0008152 IEA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    programmed cell death GO:0012501 TAS
    apoptotic process GO:0006915 TAS
    synaptic target recognition GO:0008039 IEA
    organelle organization GO:0006996 TAS
    positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway GO:1900740 TAS
Subcellular Localization
    ciliary tip GO:0097542 TAS
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    nucleus GO:0005634 IDA
    membrane GO:0016020 IDA
    primary cilium GO:0072372 TAS
    dynein complex GO:0030286 IEA
    plasma membrane GO:0005886 TAS
    centrosome GO:0005813 IDA
    myosin complex GO:0016459 ISS
 Experiment description of studies that identified DYNLL2 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DYNLL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SHANK2 22941
Affinity Capture-Western Homo sapiens
2 DLGAP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 DLG2 1740
Affinity Capture-Western Homo sapiens
4 HOMER3 9454
Two-hybrid Homo sapiens
5 DNAL4  
Two-hybrid Homo sapiens
6 BMF  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 DYNLL2 140735
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
8 EWSR1 2130
Two-hybrid Homo sapiens
9 DLG4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 BCL2L11  
Reconstituted Complex Homo sapiens
11 MYO5A 4644
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 KIAA0514  
Two-hybrid Homo sapiens
13 THAP10  
Two-hybrid Homo sapiens
14 DNCI1  
Two-hybrid Homo sapiens
15 GPHN 10243
Invitro Homo sapiens
16 XIAP  
Two-hybrid Homo sapiens
View the network image/svg+xml



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