Gene description for NRF1
Gene name nuclear respiratory factor 1
Gene symbol NRF1
Other names/aliases ALPHA-PAL
Species Homo sapiens
 Database cross references - NRF1
ExoCarta ExoCarta_4899
Entrez Gene 4899
HGNC 7996
MIM 600879
UniProt Q16656  
 NRF1 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for NRF1
Molecular Function
    protein homodimerization activity GO:0042803 ISS
    protein binding GO:0005515 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IMP
    core promoter sequence-specific DNA binding GO:0001046 ISS
Biological Process
    response to electrical stimulus GO:0051602 IEA
    organelle organization GO:0006996 TAS
    generation of precursor metabolites and energy GO:0006091 TAS
    mitochondrion organization GO:0007005 TAS
    response to lipopolysaccharide GO:0032496 IEA
    transcription, DNA-templated GO:0006351 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    organ regeneration GO:0031100 IEA
    response to hypoxia GO:0001666 IEA
    response to folic acid GO:0051593 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    response to estradiol GO:0032355 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    nucleoplasm GO:0005654 TAS
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 ISS
 Experiment description of studies that identified NRF1 in exosomes
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NRF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPRC1  
Invivo Homo sapiens
Invitro Homo sapiens
2 Dynll1 56455
Two-hybrid Mus musculus
3 Tctex1  
Two-hybrid Mus musculus
4 MAFF  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
6 DYNLL1 8655
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which NRF1 is involved
PPARA activates gene expression TAS Reactome
Transcriptional activation of mitochondrial biogenesis TAS Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here