Gene description for CSNK2B
Gene name casein kinase 2, beta polypeptide
Gene symbol CSNK2B
Other names/aliases CK2B
CK2N
CSK2B
G5A
Species Homo sapiens
 Database cross references - CSNK2B
ExoCarta ExoCarta_1460
Entrez Gene 1460
HGNC 2460
MIM 115441
UniProt P67870  
 CSNK2B identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for CSNK2B
Molecular Function
    chromatin binding GO:0003682 IDA
    protein kinase regulator activity GO:0019887 NAS
    transcription factor binding GO:0008134 IPI
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    protein domain specific binding GO:0019904 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    receptor binding GO:0005102 IPI
Biological Process
    mitotic cell cycle GO:0000278 TAS
    axon guidance GO:0007411 TAS
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of blood vessel endothelial cell migration GO:0043537 IDA
    positive regulation of activin receptor signaling pathway GO:0032927 IMP
    adiponectin-activated signaling pathway GO:0033211 IDA
    protein phosphorylation GO:0006468 TAS
    regulation of protein kinase activity GO:0045859 NAS
    positive regulation of pathway-restricted SMAD protein phosphorylation GO:0010862 IDA
    endothelial tube morphogenesis GO:0061154 IMP
    signal transduction GO:0007165 TAS
    negative regulation of cell proliferation GO:0008285 TAS
    cellular protein complex assembly GO:0043623 NAS
    regulation of DNA binding GO:0051101 NAS
Subcellular Localization
    PcG protein complex GO:0031519 IDA
    plasma membrane GO:0005886 IDA
    protein kinase CK2 complex GO:0005956 NAS
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified CSNK2B in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSNK2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCRS1  
Affinity Capture-MS Homo sapiens
2 CSNK2B 1460
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
3 Rps6kb1  
Two-hybrid Rattus norvegicus
4 FGF2 2247
Reconstituted Complex Homo sapiens
5 TOP2B 7155
Invitro Homo sapiens
6 CD163 9332
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 BTF3 689
Invitro Homo sapiens
Two-hybrid Homo sapiens
8 MBD4  
Two-hybrid Homo sapiens
9 APC  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 BRCA1 672
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 PRKCZ 5590
Affinity Capture-Western Homo sapiens
12 RPS6KB1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 CSNK2A1 1457
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
14 CDKN1A  
Reconstituted Complex Homo sapiens
15 GADD45GIP1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 SHMT2 6472
Invitro Homo sapiens
17 SHMT1 6470
Invitro Homo sapiens
18 NCF1  
Invivo Homo sapiens
19 ATF1  
Reconstituted Complex Homo sapiens
20 LYN 4067
Two-hybrid Homo sapiens
21 RNF7  
Invitro Homo sapiens
22 RPL5 6125
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 RNPS1 10921
Affinity Capture-MS Homo sapiens
24 NOLC1 9221
Invivo Homo sapiens
Invitro Homo sapiens
25 CSNK2A2 1459
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
26 TP53  
Invivo Homo sapiens
Invitro Homo sapiens
27 LYST 1130
Invitro Homo sapiens
Two-hybrid Homo sapiens
28 MAGEB2 4113
Affinity Capture-MS Homo sapiens
29 ARAF 369
Two-hybrid Homo sapiens
30 CDC34  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
31 FGF1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
32 FAF1 11124
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
33 HNRNPA2B1 3181
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CSNK2B is involved
PathwayEvidenceSource
Condensation of Prometaphase Chromosomes TAS Reactome
Signal transduction by L1 TAS Reactome
WNT mediated activation of DVL TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here