Gene description for SNX9
Gene name sorting nexin 9
Gene symbol SNX9
Other names/aliases SDP1
SH3PX1
SH3PXD3A
WISP
Species Homo sapiens
 Database cross references - SNX9
ExoCarta ExoCarta_51429
Entrez Gene 51429
HGNC 14973
MIM 605952
UniProt Q9Y5X1  
 SNX9 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for SNX9
Molecular Function
    protein binding GO:0005515 IPI
    Arp2/3 complex binding GO:0071933 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    phosphatidylinositol binding GO:0035091 IDA
    protein homodimerization activity GO:0042803 IPI
    1-phosphatidylinositol binding GO:0005545 IDA
Biological Process
    endocytosis GO:0006897 IMP
    positive regulation of protein oligomerization GO:0032461 IDA
    cleavage furrow formation GO:0036089 IMP
    membrane tubulation GO:0097320 IDA
    endosomal transport GO:0016197 IMP
    intracellular protein transport GO:0006886 IMP
    positive regulation of membrane protein ectodomain proteolysis GO:0051044 IDA
    receptor-mediated endocytosis GO:0006898 IMP
    positive regulation of protein kinase activity GO:0045860 IDA
    mitotic nuclear division GO:0007067 IEA
    lipid tube assembly GO:0060988 IDA
    vesicle organization GO:0016050 IBA
    positive regulation of GTPase activity GO:0043547 IDA
    mitotic cytokinesis GO:0000281 IMP
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    endosome GO:0005768 IBA
    trans-Golgi network GO:0005802 IDA
    clathrin-coated vesicle GO:0030136 IDA
    plasma membrane GO:0005886 IDA
    extracellular exosome GO:0070062 IDA
    ruffle GO:0001726 IEA
    cytoplasmic vesicle membrane GO:0030659 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SNX9 in exosomes
1
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
5
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SNX9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPHOSPH6  
Affinity Capture-MS Homo sapiens
2 CLTC 1213
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 DNM2 1785
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 MPP6 51678
Two-hybrid Homo sapiens
5 ADAM9 8754
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 ADAM15 8751
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SNX9 is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis TAS Reactome





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