Experiment description of studies that identified CPNE5 in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 13 MISEV standards ✔ IEM Biophysical techniques ✔ Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15326289     Organism Homo sapiens Experiment description Identification and proteomic profiling of exosomes in human urine. Authors "Pisitkun T, Shen RF, Knepper MA" Journal name PNAS Publication year 2004 Sample Urine Sample name Urine - Normal Isolation/purification methods Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

8 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

9 Experiment ID 196 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal high density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 197 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal low density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for CPNE5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GATAD2A 54815 Affinity Capture-MS Homo sapiens 2 UTRN 7402 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 GATAD2B 57459 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 NCK1 4690 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 CLINT1 9685 Affinity Capture-MS Homo sapiens 6 MTA2 9219 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 DHRS7 51635 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 MBD3 53615 Affinity Capture-MS Homo sapiens 9 RABEP2 79874 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 MTA1 9112 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 DSCC1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 RABGEF1 27342 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 UBR4 23352 Affinity Capture-MS Homo sapiens 14 GEMIN5 25929 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 RNF6   Affinity Capture-MS Homo sapiens 16 CPNE9   Affinity Capture-MS Homo sapiens 17 SNX9 51429 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 VHL   Two-hybrid Homo sapiens 19 CHD4 1108 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 SAE1 10055 Affinity Capture-MS Homo sapiens 21 NCK2 8440 Affinity Capture-MS Homo sapiens 22 SATB2   Affinity Capture-MS Homo sapiens 23 ANKRD52 283373 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 UBA2 10054 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 USO1 8615 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 DTNB 1838 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 RAD54B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 BICD2 23299 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 NAPG 8774 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 MBNL3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 SNTB2 6645 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 FAM114A2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 WDHD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 RAD21 5885 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 UROD 7389 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which CPNE5 is involved

No pathways found