Gene description for BRD4
Gene name bromodomain containing 4
Gene symbol BRD4
Other names/aliases CAP
HUNK1
HUNKI
MCAP
Species Homo sapiens
 Database cross references - BRD4
ExoCarta ExoCarta_23476
Vesiclepedia VP_23476
Entrez Gene 23476
HGNC 13575
MIM 608749
UniProt O60885  
 BRD4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for BRD4
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    p53 binding GO:0002039 IDA
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    transcription coregulator activity GO:0003712 IMP
    transcription coactivator activity GO:0003713 IMP
    protein serine/threonine kinase activity GO:0004674 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IMP
    enzyme binding GO:0019899 IPI
    histone binding GO:0042393 IBA
    lysine-acetylated histone binding GO:0070577 IDA
    RNA polymerase II C-terminal domain binding GO:0099122 IDA
    P-TEFb complex binding GO:0106140 IDA
    histone reader activity GO:0140566 IDA
Biological Process
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    DNA damage response GO:0006974 IEA
    DNA damage response GO:0006974 IMP
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IMP
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IDA
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    negative regulation by host of viral transcription GO:0043922 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    regulation of inflammatory response GO:0050727 IDA
    negative regulation of DNA damage checkpoint GO:2000002 IMP
    positive regulation of T-helper 17 cell lineage commitment GO:2000330 ISS
Subcellular Localization
    chromatin GO:0000785 IBA
    condensed nuclear chromosome GO:0000794 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 IDA
 Experiment description of studies that identified BRD4 in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for BRD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
2 HIST1H4K 8362
Affinity Capture-MS Homo sapiens
3 HDAC2 3066
Affinity Capture-Western Homo sapiens
4 MED17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 AATF  
Affinity Capture-MS Homo sapiens
6 TOP2B 7155
Affinity Capture-MS Homo sapiens
7 MED29  
Affinity Capture-MS Homo sapiens
8 ACTC1 70
Proximity Label-MS Homo sapiens
9 BRCA1 672
Negative Genetic Homo sapiens
10 BRD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PRPF40A 55660
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 SMARCD2 6603
Affinity Capture-MS Homo sapiens
13 FGFR3 2261
Negative Genetic Homo sapiens
14 GPATCH4 54865
Affinity Capture-MS Homo sapiens
15 HIST1H4E 8367
Affinity Capture-MS Homo sapiens
16 RPL7A 6130
Cross-Linking-MS (XL-MS) Homo sapiens
17 CHAF1A  
Affinity Capture-MS Homo sapiens
18 CDK2AP1  
Affinity Capture-MS Homo sapiens
19 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
20 ATAD5  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RPS9 6203
Affinity Capture-MS Homo sapiens
22 RPF2 84154
Affinity Capture-MS Homo sapiens
23 CROCC 9696
Affinity Capture-MS Homo sapiens
24 HERC2 8924
Affinity Capture-MS Homo sapiens
25 AMD1  
Affinity Capture-MS Homo sapiens
26 PSMG1 8624
Affinity Capture-MS Homo sapiens
27 MECP2 4204
Affinity Capture-MS Homo sapiens
28 WDR5 11091
Affinity Capture-Western Homo sapiens
29 MTA2 9219
Affinity Capture-Western Homo sapiens
30 CKM  
Affinity Capture-MS Homo sapiens
31 SUV420H1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HEXIM1 10614
Affinity Capture-Western Homo sapiens
33 SPDYE7P  
Affinity Capture-MS Homo sapiens
34 GPSM1 26086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
36 UTP14A 10813
Affinity Capture-MS Homo sapiens
37 TXNL4B  
Affinity Capture-MS Homo sapiens
38 RFC5 5985
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
39 C17orf49 124944
Affinity Capture-MS Homo sapiens
40 LDHC 3948
Affinity Capture-MS Homo sapiens
41 CBX5 23468
Affinity Capture-MS Homo sapiens
42 CBX6  
Affinity Capture-MS Homo sapiens
43 BANF1 8815
Affinity Capture-MS Homo sapiens
44 RFC4 5984
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
45 MED25  
Affinity Capture-MS Homo sapiens
46 WHSC1L1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RRP1B 23076
Affinity Capture-MS Homo sapiens
48 CAPN1 823
Affinity Capture-MS Homo sapiens
49 GTPBP4 23560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 TRIM63  
Two-hybrid Homo sapiens
51 HTRA1 5654
Affinity Capture-MS Homo sapiens
52 CCNT1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 PTAR1 375743
Synthetic Lethality Homo sapiens
54 RING1 6015
Affinity Capture-MS Homo sapiens
55 ASXL3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 ACTL6A 86
Affinity Capture-MS Homo sapiens
57 C1orf226  
Affinity Capture-MS Homo sapiens
58 MED26  
Affinity Capture-MS Homo sapiens
59 YWHAZ 7534
Co-localization Homo sapiens
60 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RPL7L1 285855
Affinity Capture-MS Homo sapiens
62 RBBP4 5928
Affinity Capture-MS Homo sapiens
63 SNX9 51429
Affinity Capture-MS Homo sapiens
64 HIST1H4B 8366
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 NUCB1 4924
Affinity Capture-MS Homo sapiens
67 ZMYND8 23613
Affinity Capture-MS Homo sapiens
68 PHC1  
Affinity Capture-MS Homo sapiens
69 RPL35A 6165
Affinity Capture-MS Homo sapiens
70 HSPA1A 3303
Affinity Capture-MS Homo sapiens
71 MED23 9439
Affinity Capture-MS Homo sapiens
72 SIRT5 23408
Affinity Capture-MS Homo sapiens
73 HIST1H4I 8294
Affinity Capture-MS Homo sapiens
74 HEATR1 55127
Affinity Capture-MS Homo sapiens
75 SUPT16H 11198
Affinity Capture-MS Homo sapiens
76 MED14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 GNL3 26354
Affinity Capture-MS Homo sapiens
78 ASUN  
Affinity Capture-MS Homo sapiens
79 NUDT21 11051
Affinity Capture-MS Homo sapiens
80 C8orf33 65265
Two-hybrid Homo sapiens
81 FASN 2194
Negative Genetic Homo sapiens
82 KAT8  
Co-localization Homo sapiens
83 CHTOP  
Affinity Capture-MS Homo sapiens
84 RRS1 23212
Affinity Capture-MS Homo sapiens
85 RBM25 58517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 RUVBL2 10856
Affinity Capture-MS Homo sapiens
87 TCERG1 10915
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
88 KDM1B  
Affinity Capture-MS Homo sapiens
89 SRSF9 8683
Affinity Capture-MS Homo sapiens
90 TBL3 10607
Affinity Capture-MS Homo sapiens
91 TRA2B 6434
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
92 SIRT1  
Affinity Capture-MS Homo sapiens
93 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
94 PWWP2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 MED16 10025
Affinity Capture-MS Homo sapiens
96 HIST1H4L 8368
Affinity Capture-MS Homo sapiens
97 BCL2  
Co-localization Homo sapiens
98 INA 9118
Affinity Capture-MS Homo sapiens
99 HIST1H4J 8363
Affinity Capture-MS Homo sapiens
100 CEP78 84131
Affinity Capture-MS Homo sapiens
101 INTS3 65123
Affinity Capture-MS Homo sapiens
102 DNMT1 1786
Affinity Capture-MS Homo sapiens
103 MRPL28 10573
Affinity Capture-MS Homo sapiens
104 NVL  
Affinity Capture-MS Homo sapiens
105 PRKAA1 5562
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
106 KPNA3 3839
Affinity Capture-MS Homo sapiens
107 TOR1A 1861
Affinity Capture-MS Homo sapiens
108 BRDT  
Affinity Capture-MS Homo sapiens
109 TRRAP 8295
Affinity Capture-MS Homo sapiens
110 NOS1AP  
Affinity Capture-MS Homo sapiens
111 NOVA2  
Affinity Capture-MS Homo sapiens
112 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
113 NOP56 10528
Affinity Capture-MS Homo sapiens
114 PTEN 5728
Negative Genetic Homo sapiens
115 PWP2 5822
Affinity Capture-MS Homo sapiens
116 PSIP1 11168
Affinity Capture-MS Homo sapiens
117 XRCC1 7515
Affinity Capture-MS Homo sapiens
118 HSPA2 3306
Affinity Capture-MS Homo sapiens
119 NOLC1 9221
Affinity Capture-MS Homo sapiens
120 RRP12 23223
Affinity Capture-MS Homo sapiens
121 RFC3 5983
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
122 MED27  
Affinity Capture-MS Homo sapiens
123 MDC1  
Affinity Capture-MS Homo sapiens
124 HIST4H4 121504
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
125 FIBP 9158
Affinity Capture-MS Homo sapiens
126 TUBB6 84617
Affinity Capture-MS Homo sapiens
127 CST1  
Affinity Capture-MS Homo sapiens
128 RPL34 6164
Affinity Capture-MS Homo sapiens
129 MED4 29079
Affinity Capture-MS Homo sapiens
130 JMJD6 23210
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
131 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
132 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
133 RPL4 6124
Cross-Linking-MS (XL-MS) Homo sapiens
134 WHSC1 7468
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 TBR1  
Affinity Capture-MS Homo sapiens
136 ACIN1 22985
Affinity Capture-MS Homo sapiens
137 VRK1 7443
Affinity Capture-MS Homo sapiens
138 UBP1 7342
Affinity Capture-MS Homo sapiens
139 NAA35 60560
Affinity Capture-MS Homo sapiens
140 INTS7  
Affinity Capture-MS Homo sapiens
141 UBTF 7343
Affinity Capture-MS Homo sapiens
142 SPDYE4  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
143 IARS 3376
Cross-Linking-MS (XL-MS) Homo sapiens
144 POLB 5423
Affinity Capture-MS Homo sapiens
145 TFR2 7036
Affinity Capture-MS Homo sapiens
146 HMGN3  
Affinity Capture-MS Homo sapiens
147 TUBB 203068
Affinity Capture-MS Homo sapiens
148 CCNC  
Affinity Capture-MS Homo sapiens
149 SCML1  
Affinity Capture-MS Homo sapiens
150 BRD8 10902
Affinity Capture-MS Homo sapiens
151 PHIP 55023
Affinity Capture-MS Homo sapiens
152 CDH15 1013
Affinity Capture-MS Homo sapiens
153 EGFR 1956
Negative Genetic Homo sapiens
Co-localization Homo sapiens
154 FAM90A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
155 WEE1 7465
Synthetic Lethality Homo sapiens
156 ZNF740  
Affinity Capture-MS Homo sapiens
157 CCNT2  
Affinity Capture-Western Homo sapiens
158 DMAP1 55929
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
159 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 MYC  
Dosage Lethality Homo sapiens
Affinity Capture-Western Homo sapiens
161 BAG1 573
Affinity Capture-MS Homo sapiens
162 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
163 NIPBL 25836
Affinity Capture-MS Homo sapiens
164 HIST1H4F 8361
Affinity Capture-MS Homo sapiens
165 RCN2 5955
Affinity Capture-MS Homo sapiens
166 Junb  
Affinity Capture-MS Mus musculus
167 BMI1  
Affinity Capture-MS Homo sapiens
168 NELFB 25920
Affinity Capture-MS Homo sapiens
169 BRD9 65980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 FTSJ3 117246
Affinity Capture-MS Homo sapiens
171 MLKL 197259
Affinity Capture-MS Homo sapiens
172 MED11  
Affinity Capture-MS Homo sapiens
173 TAF6 6878
Affinity Capture-MS Homo sapiens
174 FGF11  
Affinity Capture-MS Homo sapiens
175 SPOP  
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
176 H3F3A 3020
Reconstituted Complex Homo sapiens
177 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
178 SIPA1 6494
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
179 CALU 813
Affinity Capture-MS Homo sapiens
180 KNOP1 400506
Affinity Capture-MS Homo sapiens
181 NELFA  
Affinity Capture-MS Homo sapiens
182 CHD1 1105
Affinity Capture-MS Homo sapiens
183 NIFK 84365
Affinity Capture-MS Homo sapiens