Gene description for CHD1
Gene name chromodomain helicase DNA binding protein 1
Gene symbol CHD1
Other names/aliases -
Species Homo sapiens
 Database cross references - CHD1
ExoCarta ExoCarta_1105
Vesiclepedia VP_1105
Entrez Gene 1105
HGNC 1915
MIM 602118
UniProt O14646  
 CHD1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for CHD1
Molecular Function
    DNA binding GO:0003677 IBA
    chromatin binding GO:0003682 IBA
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IBA
    methylated histone binding GO:0035064 IDA
    methylated histone binding GO:0035064 IMP
    double-stranded DNA helicase activity GO:0036121 IEA
    histone binding GO:0042393 IBA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent chromatin remodeler activity GO:0140658 IBA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    chromatin remodeling GO:0006338 IMP
    DNA duplex unwinding GO:0032508 IEA
    nucleosome organization GO:0034728 IBA
    positive regulation by host of viral transcription GO:0043923 IMP
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nuclear chromosome GO:0000228 IDA
    chromatin GO:0000785 IBA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified CHD1 in exosomes
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for CHD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 ZRANB1 54764
Affinity Capture-MS Homo sapiens
3 FBL 2091
Proximity Label-MS Homo sapiens
4 Cbx1  
Affinity Capture-MS Mus musculus
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 TMPO 7112
Affinity Capture-MS Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 ZBTB2 57621
Affinity Capture-MS Homo sapiens
9 MLLT1 4298
Affinity Capture-MS Homo sapiens
10 CTR9 9646
Co-fractionation Homo sapiens
11 SLFN11 91607
Affinity Capture-MS Homo sapiens
12 PARP1 142
Proximity Label-MS Homo sapiens
13 CENPA  
Proximity Label-MS Homo sapiens
14 COIL  
Proximity Label-MS Homo sapiens
15 DNAJC8 22826
Proximity Label-MS Homo sapiens
16 BCLAF1 9774
Two-hybrid Homo sapiens
17 FGF11  
Affinity Capture-MS Homo sapiens
18 SIRT7  
Affinity Capture-MS Homo sapiens
19 SPP1 6696
Two-hybrid Homo sapiens
20 CHMP4C 92421
Affinity Capture-MS Homo sapiens
21 USP36  
Affinity Capture-MS Homo sapiens
22 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
23 Pparg 19016
Affinity Capture-MS Mus musculus
24 MEN1 4221
Affinity Capture-MS Homo sapiens
25 NOP56 10528
Proximity Label-MS Homo sapiens
26 DHX8 1659
Proximity Label-MS Homo sapiens
27 Atrx  
Affinity Capture-MS Mus musculus
28 RPL10 6134
Affinity Capture-MS Homo sapiens
29 KIF20A 10112
Affinity Capture-MS Homo sapiens
30 CBX3 11335
Proximity Label-MS Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 BTRC 8945
Affinity Capture-Western Homo sapiens
33 HDAC1 3065
Affinity Capture-Western Homo sapiens
34 HIST1H3A 8350
Proximity Label-MS Homo sapiens
Co-crystal Structure Homo sapiens
35 KIF23 9493
Affinity Capture-MS Homo sapiens
36 SRSF3 6428
Two-hybrid Homo sapiens
37 HIST3H3 8290
Protein-peptide Homo sapiens
38 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
39 DNAJB6 10049
Proximity Label-MS Homo sapiens
40 DHX40  
Proximity Label-MS Homo sapiens
41 SAFB 6294
Two-hybrid Homo sapiens
42 RTF1 23168
Co-fractionation Homo sapiens
43 FASN 2194
Negative Genetic Homo sapiens
44 DDX23 9416
Proximity Label-MS Homo sapiens
45 NCOR1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
46 CHMP4B 128866
Affinity Capture-MS Homo sapiens
47 YAP1 10413
Affinity Capture-MS Homo sapiens
48 Tpx2  
Affinity Capture-MS Mus musculus
49 ARRB1 408
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 INO80B 83444
Affinity Capture-MS Homo sapiens
52 CIT 11113
Affinity Capture-MS Homo sapiens
53 FBXW7  
Affinity Capture-MS Homo sapiens
54 PRC1 9055
Affinity Capture-MS Homo sapiens
55 CUL7 9820
Affinity Capture-MS Homo sapiens
56 NPM1 4869
Affinity Capture-MS Homo sapiens
57 HNRNPU 3192
Affinity Capture-MS Homo sapiens
58 POLD1 5424
Affinity Capture-MS Homo sapiens
59 Mad2l2  
Affinity Capture-MS Mus musculus
60 POLR1E  
Proximity Label-MS Homo sapiens
61 PAF1 54623
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
62 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 LDLR 3949
Negative Genetic Homo sapiens
64 SSRP1 6749
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
65 HMGA1 3159
Affinity Capture-Western Homo sapiens
66 Cul4a  
Affinity Capture-MS Mus musculus
67 BRD3 8019
Affinity Capture-MS Homo sapiens
68 ATP5O 539
Cross-Linking-MS (XL-MS) Homo sapiens
69 CEBPA  
Affinity Capture-Western Homo sapiens
70 FGF13  
Affinity Capture-MS Homo sapiens
71 KRAS 3845
Negative Genetic Homo sapiens
72 DOCK9 23348
Affinity Capture-MS Homo sapiens
73 BRD4 23476
Affinity Capture-MS Homo sapiens
74 RPA3 6119
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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