Gene description for DHX8
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 8
Gene symbol DHX8
Other names/aliases DDX8
HRH1
PRP22
PRPF22
Species Homo sapiens
 Database cross references - DHX8
ExoCarta ExoCarta_1659
Vesiclepedia VP_1659
Entrez Gene 1659
HGNC 2749
MIM 600396
UniProt Q14562  
 DHX8 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for DHX8
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA helicase activity GO:0003724 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP-dependent activity, acting on RNA GO:0008186 TAS
    ATP hydrolysis activity GO:0016887 IEA
    identical protein binding GO:0042802 IPI
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    chromatin remodeling GO:0006338 IEA
    RNA processing GO:0006396 TAS
    RNA splicing GO:0008380 TAS
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 TAS
    cytosol GO:0005829 IDA
    nuclear body GO:0016604 IDA
    U2-type catalytic step 2 spliceosome GO:0071007 IDA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified DHX8 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHX8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MORC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CCDC12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 INTS4  
Proximity Label-MS Homo sapiens
4 SLFN11 91607
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 FAM208A  
Proximity Label-MS Homo sapiens
7 CCDC94  
Proximity Label-MS Homo sapiens
8 HNRNPC 3183
Affinity Capture-MS Homo sapiens
9 ZC3H14  
Proximity Label-MS Homo sapiens
10 RPL26L1 51121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 POLDIP3 84271
Proximity Label-MS Homo sapiens
12 DGCR14  
Proximity Label-MS Homo sapiens
13 COIL  
Proximity Label-MS Homo sapiens
14 ZNF346  
Affinity Capture-MS Homo sapiens
15 PCF11 51585
Proximity Label-MS Homo sapiens
16 PPIE 10450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SRPK2 6733
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PPIL4  
Proximity Label-MS Homo sapiens
19 PRPF38A 84950
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 RPL13A 23521
Affinity Capture-MS Homo sapiens
21 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
22 THRAP3 9967
Proximity Label-MS Homo sapiens
23 DHX8 1659
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
24 RBM27  
Proximity Label-MS Homo sapiens
25 Ccdc12  
Affinity Capture-MS Mus musculus
26 C9orf78 51759
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 UBC 7316
Reconstituted Complex Homo sapiens
28 RPL14 9045
Affinity Capture-MS Homo sapiens
29 XRN2 22803
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
30 CCDC137  
Affinity Capture-MS Homo sapiens
31 CAND1 55832
Affinity Capture-MS Homo sapiens
32 RSBN1  
Proximity Label-MS Homo sapiens
33 AQR 9716
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SYF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CWC25 54883
Proximity Label-MS Homo sapiens
36 PRPF40A 55660
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
37 SNRPD2 6633
Proximity Label-MS Homo sapiens
38 RPS3 6188
Affinity Capture-MS Homo sapiens
39 USP36  
Affinity Capture-MS Homo sapiens
40 TCEB3 6924
Proximity Label-MS Homo sapiens
41 CCNK  
Proximity Label-MS Homo sapiens
42 DDX23 9416
Proximity Label-MS Homo sapiens
43 GPATCH4 54865
Proximity Label-MS Homo sapiens
44 RPL10A 4736
Affinity Capture-MS Homo sapiens
45 RNPS1 10921
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
46 TAF9 6880
Co-fractionation Homo sapiens
47 CRNKL1 51340
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GPATCH1  
Proximity Label-MS Homo sapiens
49 AFF4 27125
Proximity Label-MS Homo sapiens
50 LUC7L2 51631
Two-hybrid Homo sapiens
51 Snw1  
Affinity Capture-MS Mus musculus
52 ISY1 57461
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ZNF638 27332
Proximity Label-MS Homo sapiens
54 WDR83  
Proximity Label-MS Homo sapiens
55 Isy1 57905
Affinity Capture-MS Mus musculus
56 CDK12 51755
Proximity Label-MS Homo sapiens
57 PRC1 9055
Affinity Capture-MS Homo sapiens
58 CHCHD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SRRM2 23524
Proximity Label-MS Homo sapiens
60 SNRPC 6631
Affinity Capture-MS Homo sapiens
61 SAFB 6294
Proximity Label-MS Homo sapiens
62 OBSL1 23363
Affinity Capture-MS Homo sapiens
63 MORC1  
Affinity Capture-MS Homo sapiens
64 ZNF451 26036
Proximity Label-MS Homo sapiens
65 RPL6 6128
Affinity Capture-MS Homo sapiens
66 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 DDX18 8886
Proximity Label-MS Homo sapiens
68 NUDCD1 84955
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 DDX41 51428
Proximity Label-MS Homo sapiens
70 MECP2 4204
Affinity Capture-MS Homo sapiens
71 TTC14  
Proximity Label-MS Homo sapiens
72 THOC1 9984
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 SLU7  
Proximity Label-MS Homo sapiens
74 RPL19 6143
Affinity Capture-MS Homo sapiens
75 AHCYL1 10768
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 KIF14 9928
Affinity Capture-MS Homo sapiens
77 LIN28A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 WRN 7486
Proximity Label-MS Homo sapiens
79 KANSL1  
Co-fractionation Homo sapiens
80 CHD1 1105
Proximity Label-MS Homo sapiens
81 Bcas2  
Affinity Capture-MS Mus musculus
82 PRPF6 24148
Proximity Label-MS Homo sapiens
83 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 SCAF1  
Proximity Label-MS Homo sapiens
85 USP12 219333
Affinity Capture-MS Homo sapiens
86 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
87 RTF1 23168
Proximity Label-MS Homo sapiens
88 CASZ1  
Proximity Label-MS Homo sapiens
89 PARK2  
Affinity Capture-MS Homo sapiens
90 RBM28 55131
Affinity Capture-MS Homo sapiens
91 Crnkl1  
Affinity Capture-MS Mus musculus
92 RBM7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
93 PNN 5411
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
94 RSRC2  
Proximity Label-MS Homo sapiens
95 SRSF6 6431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 PURG  
Affinity Capture-MS Homo sapiens
97 NOP2 4839
Affinity Capture-MS Homo sapiens
98 CBX3 11335
Proximity Label-MS Homo sapiens
99 SNRNP200 23020
Proximity Label-MS Homo sapiens
100 RPL5 6125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 PPIL2  
Proximity Label-MS Homo sapiens
102 SNRPB 6628
Affinity Capture-MS Homo sapiens
103 RPL18A 6142
Affinity Capture-MS Homo sapiens
104 SNRPD1 6632
Proximity Label-MS Homo sapiens
105 SRSF11 9295
Proximity Label-MS Homo sapiens
106 NTRK1 4914
Affinity Capture-MS Homo sapiens
107 RPL31 6160
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 MYPN 84665
Co-fractionation Homo sapiens
109 IK 3550
Proximity Label-MS Homo sapiens
110 SMAD3 4088
Affinity Capture-MS Homo sapiens
111 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
112 RBMX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
113 RPL36 25873
Affinity Capture-MS Homo sapiens
114 SMAD2 4087
Affinity Capture-MS Homo sapiens
115 SCAF11  
Proximity Label-MS Homo sapiens
116 ENO1 2023
Affinity Capture-RNA Homo sapiens
117 HECTD1 25831
Affinity Capture-MS Homo sapiens
118 CTR9 9646
Proximity Label-MS Homo sapiens
119 ZFC3H1 196441
Proximity Label-MS Homo sapiens
120 SRSF4 6429
Affinity Capture-MS Homo sapiens
121 MAGEB2 4113
Affinity Capture-MS Homo sapiens
122 NKTR  
Proximity Label-MS Homo sapiens
123 CDX1  
Affinity Capture-MS Homo sapiens
124 FGF13  
Affinity Capture-MS Homo sapiens
125 SRPK1 6732
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 BCOR  
Proximity Label-MS Homo sapiens
127 RBM10  
Proximity Label-MS Homo sapiens
128 SNRPB2 6629
Affinity Capture-MS Homo sapiens
129 RSL1D1 26156
Affinity Capture-MS Homo sapiens
130 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
131 BCLAF1 9774
Proximity Label-MS Homo sapiens
132 DDX59 83479
Proximity Label-MS Homo sapiens
133 RBBP6 5930
Proximity Label-MS Homo sapiens
134 RPRD2  
Proximity Label-MS Homo sapiens
135 RBM5  
Proximity Label-MS Homo sapiens
136 RPL32 6161
Affinity Capture-MS Homo sapiens
137 SETD2  
Proximity Label-MS Homo sapiens
138 PRPF4B 8899
Proximity Label-MS Homo sapiens
139 SUGP1  
Proximity Label-MS Homo sapiens
140 ZNF830  
Proximity Label-MS Homo sapiens
141 CUL3 8452
Affinity Capture-MS Homo sapiens
142 CDC73  
Proximity Label-MS Homo sapiens
143 CXorf56  
Affinity Capture-MS Homo sapiens
144 CHERP 10523
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
145 RPL15 6138
Affinity Capture-MS Homo sapiens
146 ZCCHC10  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
147 ECT2 1894
Affinity Capture-MS Homo sapiens
148 SIRT7  
Affinity Capture-MS Homo sapiens
149 CD2BP2 10421
Affinity Capture-MS Homo sapiens
150 PLRG1 5356
Proximity Label-MS Homo sapiens
151 SYMPK 8189
Proximity Label-MS Homo sapiens
152 SRSF1 6426
Affinity Capture-MS Homo sapiens
153 WDR33 55339
Proximity Label-MS Homo sapiens
154 NUDT21 11051
Proximity Label-MS Homo sapiens
155 BLM 641
Proximity Label-MS Homo sapiens
156 RBM41  
Proximity Label-MS Homo sapiens
157 SAP30BP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
158 XAB2 56949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 YLPM1 56252
Proximity Label-MS Homo sapiens
160 RPL3 6122
Affinity Capture-MS Homo sapiens
161 TNPO3 23534
Affinity Capture-MS Homo sapiens
162 PRPF3  
Proximity Label-MS Homo sapiens
163 TSSC4  
Affinity Capture-MS Homo sapiens
164 ZC3H11A  
Affinity Capture-MS Homo sapiens
165 SLTM 79811
Proximity Label-MS Homo sapiens
166 SART1 9092
Proximity Label-MS Homo sapiens
167 NXF1 10482
Affinity Capture-RNA Homo sapiens
168 BUD13  
Proximity Label-MS Homo sapiens
169 RBM6 10180
Proximity Label-MS Homo sapiens
170 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
171 PRCC  
Proximity Label-MS Homo sapiens
172 NSRP1  
Proximity Label-MS Homo sapiens
173 KIAA1429 25962
Proximity Label-MS Homo sapiens
174 MFAP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
175 PRPF19 27339
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
176 SPEN 23013
Proximity Label-MS Homo sapiens
177 RPA3 6119
Proximity Label-MS Homo sapiens
178 CLASRP  
Proximity Label-MS Homo sapiens
179 DLST 1743
Affinity Capture-MS Homo sapiens
180 TOP1 7150
Affinity Capture-MS Homo sapiens
181 PHF5A 84844
Co-fractionation Homo sapiens
182 DHX34 9704
Affinity Capture-MS Homo sapiens
183 FIP1L1 81608
Proximity Label-MS Homo sapiens
184 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
185 SKIV2L2 23517
Proximity Label-MS Homo sapiens
186 GPATCH8  
Proximity Label-MS Homo sapiens
187 DDRGK1 65992
Affinity Capture-MS Homo sapiens
188 CACTIN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
189 TFIP11  
Proximity Label-MS Homo sapiens
190 DOT1L 84444
Proximity Label-MS Homo sapiens
191 HIST1H1T 3010
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 RPL26 6154
Affinity Capture-MS Homo sapiens
193 FTSJ3 117246
Affinity Capture-MS Homo sapiens
194 WAC  
Proximity Label-MS Homo sapiens
195 RBM17 84991
Proximity Label-MS Homo sapiens
196 RSBN1L  
Proximity Label-MS Homo sapiens
197 NKAP 79576
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
198 U2SURP 23350
Proximity Label-MS Homo sapiens
199 DDX46 9879
Proximity Label-MS Homo sapiens
200 PAXBP1  
Proximity Label-MS Homo sapiens
201 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
202 FNBP4  
Proximity Label-MS Homo sapiens
203 SALL2  
Proximity Label-MS Homo sapiens
204 SRRM1 10250
Proximity Label-MS Homo sapiens
205 EAPP  
Affinity Capture-MS Homo sapiens
206 PAF1 54623
Proximity Label-MS Homo sapiens
207 BCAS2 10286
Affinity Capture-MS Homo sapiens
208 ZRANB2 9406
Proximity Label-MS Homo sapiens
209 CPSF6 11052
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
210 POLR1B 84172
Co-fractionation Homo sapiens
211 CWC27 10283
Proximity Label-MS Homo sapiens
212 RBM25 58517
Proximity Label-MS Homo sapiens
213 CDC40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
214 TCERG1 10915
Proximity Label-MS Homo sapiens
215 WBP11  
Proximity Label-MS Homo sapiens
216 PHRF1  
Proximity Label-MS Homo sapiens
217 RPS8 6202
Affinity Capture-MS Homo sapiens
218 SON 6651
Proximity Label-MS Homo sapiens
219 SAP18 10284
Proximity Label-MS Homo sapiens
220 TUBG1 7283
Proximity Label-MS Homo sapiens
221 SNRPA 6626
Affinity Capture-MS Homo sapiens
222 ZC3H18  
Proximity Label-MS Homo sapiens
223 LRWD1