Gene description for CWC25
Gene name CWC25 spliceosome-associated protein homolog (S. cerevisiae)
Gene symbol CWC25
Other names/aliases CCDC49
Species Homo sapiens
 Database cross references - CWC25
ExoCarta ExoCarta_54883
Vesiclepedia VP_54883
Entrez Gene 54883
HGNC 25989
UniProt Q9NXE8  
 CWC25 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for CWC25
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    U2-type spliceosomal complex GO:0005684 IBA
    nuclear speck GO:0016607 IDA
    U2-type catalytic step 1 spliceosome GO:0071006 IDA
 Experiment description of studies that identified CWC25 in exosomes
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CWC25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 KSR1  
Affinity Capture-MS Homo sapiens
4 TPX2  
Affinity Capture-MS Homo sapiens
5 LAMC3  
Two-hybrid Homo sapiens
6 ZBTB21  
Affinity Capture-MS Homo sapiens
7 MYCN  
Affinity Capture-MS Homo sapiens
8 DVL2 1856
Affinity Capture-MS Homo sapiens
9 KIF14 9928
Affinity Capture-MS Homo sapiens
10 JPH3 57338
Affinity Capture-MS Homo sapiens
11 SERF2 10169
Affinity Capture-MS Homo sapiens
12 FMR1 2332
Two-hybrid Homo sapiens
13 H2AFJ 55766
Affinity Capture-MS Homo sapiens
14 ANAPC15  
Affinity Capture-MS Homo sapiens
15 INPP5E 56623
Affinity Capture-MS Homo sapiens
16 NOP56 10528
Proximity Label-MS Homo sapiens
17 DHX8 1659
Proximity Label-MS Homo sapiens
18 TMEM14B  
Affinity Capture-MS Homo sapiens
19 CYTH4  
Affinity Capture-MS Homo sapiens
20 IFI6  
Affinity Capture-MS Homo sapiens
21 C20orf197  
Affinity Capture-MS Homo sapiens
22 CBY1  
Affinity Capture-MS Homo sapiens
23 EZH1  
Affinity Capture-MS Homo sapiens
24 SOX2  
Affinity Capture-MS Homo sapiens
25 ANKRD50 57182
Affinity Capture-MS Homo sapiens
26 DHX40  
Proximity Label-MS Homo sapiens
27 IFI27L1  
Affinity Capture-MS Homo sapiens
28 KIF13B 23303
Affinity Capture-MS Homo sapiens
29 DDX23 9416
Proximity Label-MS Homo sapiens
30 TMEM257  
Affinity Capture-MS Homo sapiens
31 TRIM21 6737
Two-hybrid Homo sapiens
32 OGT 8473
Reconstituted Complex Homo sapiens
33 GIGYF1  
Affinity Capture-MS Homo sapiens
34 CCDC85B  
Two-hybrid Homo sapiens
35 COPS6 10980
Affinity Capture-MS Homo sapiens
36 RPS6 6194
Proximity Label-MS Homo sapiens
37 GAGE5  
Affinity Capture-MS Homo sapiens
38 LPIN3 64900
Affinity Capture-MS Homo sapiens
39 CGN  
Affinity Capture-MS Homo sapiens
40 SAA1 6288
Affinity Capture-MS Homo sapiens
41 CYCS 54205
Affinity Capture-MS Homo sapiens
42 KRT34  
Two-hybrid Homo sapiens
43 KCTD3  
Affinity Capture-MS Homo sapiens
44 UQCR11  
Affinity Capture-MS Homo sapiens
45 MDM2  
Reconstituted Complex Homo sapiens
46 MALL  
Affinity Capture-MS Homo sapiens
47 SUMO1 7341
Affinity Capture-MS Homo sapiens
48 SP100 6672
Affinity Capture-MS Homo sapiens
49 COL8A1 1295
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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