Gene description for ANKRD50
Gene name ankyrin repeat domain 50
Gene symbol ANKRD50
Other names/aliases -
Species Homo sapiens
 Database cross references - ANKRD50
ExoCarta ExoCarta_57182
Vesiclepedia VP_57182
Entrez Gene 57182
HGNC 29223
UniProt Q9ULJ7  
 ANKRD50 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ANKRD50
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 IMP
Subcellular Localization
    endosome GO:0005768 IEA
 Experiment description of studies that identified ANKRD50 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANKRD50
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC14B 8555
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 RBMXL1  
Affinity Capture-MS Homo sapiens
4 PCF11 51585
Affinity Capture-MS Homo sapiens
5 UTP11L  
Affinity Capture-MS Homo sapiens
6 PRPF38A 84950
Affinity Capture-MS Homo sapiens
7 ESF1 51575
Affinity Capture-MS Homo sapiens
8 RBBP6 5930
Affinity Capture-MS Homo sapiens
9 ZC3H4 23211
Affinity Capture-MS Homo sapiens
10 BUD13  
Affinity Capture-MS Homo sapiens
11 CWC25 54883
Affinity Capture-MS Homo sapiens
12 PRPF40A 55660
Affinity Capture-MS Homo sapiens
13 APP 351
Reconstituted Complex Homo sapiens
14 ZNF414  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 VPS29 51699
Affinity Capture-MS Homo sapiens
16 KIAA1429 25962
Affinity Capture-MS Homo sapiens
17 CAPZB 832
Affinity Capture-MS Homo sapiens
18 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
19 CDK11B 984
Affinity Capture-MS Homo sapiens
20 PRPF38B 55119
Affinity Capture-MS Homo sapiens
21 ODF3  
Affinity Capture-MS Homo sapiens
22 ZNF638 27332
Affinity Capture-MS Homo sapiens
23 CDK12 51755
Affinity Capture-MS Homo sapiens
24 RAI14 26064
Affinity Capture-MS Homo sapiens
25 ZGPAT  
Affinity Capture-MS Homo sapiens
26 ARMCX3 51566
Affinity Capture-MS Homo sapiens
27 HELLS 3070
Affinity Capture-MS Homo sapiens
28 INTS6 26512
Affinity Capture-MS Homo sapiens
29 PTPN14 5784
Affinity Capture-MS Homo sapiens
30 MTDH 92140
Affinity Capture-MS Homo sapiens
31 DDX41 51428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SNX27 81609
Affinity Capture-MS Homo sapiens
33 CEP131 22994
Affinity Capture-MS Homo sapiens
34 BAZ1B 9031
Affinity Capture-MS Homo sapiens
35 ZDHHC17 23390
Two-hybrid Homo sapiens
36 VRK3 51231
Affinity Capture-MS Homo sapiens
37 SLTM 79811
Affinity Capture-MS Homo sapiens
38 RBM15B  
Affinity Capture-MS Homo sapiens
39 LYN 4067
Proximity Label-MS Homo sapiens
40 MCAM 4162
Proximity Label-MS Homo sapiens
41 HIF1AN 55662
Two-hybrid Homo sapiens
42 SAFB2 9667
Affinity Capture-MS Homo sapiens
43 ENTPD1 953
Affinity Capture-MS Homo sapiens
44 EMILIN3  
Affinity Capture-MS Homo sapiens
45 NSRP1  
Affinity Capture-MS Homo sapiens
46 SNRNP27  
Affinity Capture-MS Homo sapiens
47 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
48 LRCH3 84859
Affinity Capture-MS Homo sapiens
49 HABP4  
Affinity Capture-MS Homo sapiens
50 SFPQ 6421
Affinity Capture-MS Homo sapiens
51 C11orf57  
Affinity Capture-MS Homo sapiens
52 TLE2  
Affinity Capture-MS Homo sapiens
53 XPO1 7514
Affinity Capture-MS Homo sapiens
54 MYH14 79784
Affinity Capture-MS Homo sapiens
55 YLPM1 56252
Affinity Capture-MS Homo sapiens
56 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ZNF250  
Two-hybrid Homo sapiens
58 EEPD1 80820
Affinity Capture-MS Homo sapiens
59 RP9  
Affinity Capture-MS Homo sapiens
60 PPHLN1  
Affinity Capture-MS Homo sapiens
61 TJP2 9414
Affinity Capture-MS Homo sapiens
62 MAP9 79884
Affinity Capture-MS Homo sapiens
63 DONSON  
Affinity Capture-MS Homo sapiens
64 CACTIN  
Affinity Capture-MS Homo sapiens
65 ARGLU1  
Affinity Capture-MS Homo sapiens
66 MPRIP 23164
Affinity Capture-MS Homo sapiens
67 GRAMD1B  
Affinity Capture-MS Homo sapiens
68 KIAA1522 57648
Affinity Capture-MS Homo sapiens
69 NKAP 79576
Affinity Capture-MS Homo sapiens
70 CXorf66  
Affinity Capture-MS Homo sapiens
71 DDX46 9879
Affinity Capture-MS Homo sapiens
72 RRP36 88745
Affinity Capture-MS Homo sapiens
73 PRRC2A 7916
Affinity Capture-MS Homo sapiens
74 DIDO1  
Affinity Capture-MS Homo sapiens
75 ACSBG1  
Affinity Capture-MS Homo sapiens
76 RBM25 58517
Affinity Capture-MS Homo sapiens
77 WBP11  
Affinity Capture-MS Homo sapiens
78 PPIG 9360
Affinity Capture-MS Homo sapiens
79 PHF3  
Affinity Capture-MS Homo sapiens
80 RRP1 8568
Affinity Capture-MS Homo sapiens
81 SMURF1 57154
Biochemical Activity Homo sapiens
82 C11orf52 91894
Proximity Label-MS Homo sapiens
83 SFSWAP  
Affinity Capture-MS Homo sapiens
84 SNIP1  
Affinity Capture-MS Homo sapiens
85 ARL13B 200894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 RAB7A 7879
Proximity Label-MS Homo sapiens
87 RPL23AP32 56969
Affinity Capture-MS Homo sapiens
88 MYO1E 4643
Affinity Capture-MS Homo sapiens
89 EPHA2 1969
Proximity Label-MS Homo sapiens
90 KRAS 3845
Proximity Label-MS Homo sapiens
91 ZC3H13 23091
Affinity Capture-MS Homo sapiens
92 BMP6  
Affinity Capture-MS Homo sapiens
93 C12orf57 113246
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ANKRD50 is involved
No pathways found





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