Experiment description of studies that identified ESF1 in sEVs

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for ESF1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GNL2 29889 Affinity Capture-MS Homo sapiens 2 UBE2H 7328 Affinity Capture-MS Homo sapiens 3 CDC14B 8555 Proximity Label-MS Homo sapiens 4 RIT1 6016 Negative Genetic Homo sapiens 5 H2AFJ 55766 Affinity Capture-MS Homo sapiens 6 SENP3 26168 Affinity Capture-MS Homo sapiens 7 NOP58 51602 Co-fractionation Homo sapiens 8 ZMYND8 23613 Affinity Capture-MS Homo sapiens 9 COIL   Proximity Label-MS Homo sapiens 10 FBXO7 25793 Affinity Capture-MS Homo sapiens 11 KPNA1 3836 Affinity Capture-MS Homo sapiens 12 KIAA0020 9933 Co-fractionation Homo sapiens 13 RPSAP58 388524 Affinity Capture-MS Homo sapiens 14 HIST1H2BG 8339 Proximity Label-MS Homo sapiens 15 ACTC1 70 Proximity Label-MS Homo sapiens 16 NOP56 10528 Proximity Label-MS Homo sapiens 17 CHCHD1   Proximity Label-MS Homo sapiens 18 CSNK2A1 1457 Affinity Capture-MS Homo sapiens 19 PEBP1 5037 Co-fractionation Homo sapiens 20 UTP3 57050 Co-fractionation Homo sapiens 21 Nhsl1   Affinity Capture-MS Mus musculus 22 CENPA   Proximity Label-MS Homo sapiens 23 ANKRD50 57182 Affinity Capture-MS Homo sapiens 24 USP36   Affinity Capture-MS Homo sapiens 25 DDX23 9416 Proximity Label-MS Homo sapiens 26 KRTAP19-7   Affinity Capture-MS Homo sapiens 27 ARL3 403 Affinity Capture-MS Homo sapiens 28 RNPS1 10921 Affinity Capture-MS Homo sapiens 29 CDK2AP1   Affinity Capture-MS Homo sapiens 30 MPHOSPH10 10199 Co-fractionation Homo sapiens 31 PRC1 9055 Affinity Capture-MS Homo sapiens 32 COL8A1 1295 Affinity Capture-MS Homo sapiens 33 NPM1 4869 Affinity Capture-MS Homo sapiens 34 RPS8 6202 Affinity Capture-MS Homo sapiens 35 FBL 2091 Proximity Label-MS Homo sapiens 36 POLR1E   Proximity Label-MS Homo sapiens 37 ATP2A2 488 Cross-Linking-MS (XL-MS) Homo sapiens 38 SCD 6319 Co-fractionation Homo sapiens 39 PEA15 8682 Affinity Capture-MS Homo sapiens 40 GAGE2E 26749 Affinity Capture-MS Homo sapiens 41 AATF   Co-fractionation Homo sapiens 42 DDX18 8886 Co-fractionation Homo sapiens 43 SERF2 10169 Affinity Capture-MS Homo sapiens 44 CALR 811 Co-fractionation Homo sapiens 45 MECP2 4204 Affinity Capture-MS Homo sapiens 46 SH3BGRL 6451 Affinity Capture-MS Homo sapiens 47 CSNK2B 1460 Affinity Capture-MS Homo sapiens 48 RPS11 6205 Affinity Capture-MS Homo sapiens 49 GAR1 54433 Affinity Capture-MS Homo sapiens 50 PES1 23481 Co-fractionation Homo sapiens 51 JPH3 57338 Affinity Capture-MS Homo sapiens 52 TMEM14B   Affinity Capture-MS Homo sapiens 53 RPA4   Proximity Label-MS Homo sapiens 54 IFI27L1   Affinity Capture-MS Homo sapiens 55 CBX3 11335 Proximity Label-MS Homo sapiens 56 ACACA 31 Positive Genetic Homo sapiens 57 AKR7L 246181 Affinity Capture-MS Homo sapiens 58 Srp72   Affinity Capture-MS Mus musculus 59 C16orf58 64755 Affinity Capture-MS Homo sapiens 60 RPL31 6160 Proximity Label-MS Homo sapiens 61 ANAPC15   Affinity Capture-MS Homo sapiens 62 RPS6 6194 Proximity Label-MS Homo sapiens 63 ADAMTS1 9510 Affinity Capture-MS Homo sapiens 64 C1orf189   Affinity Capture-MS Homo sapiens 65 CD3EAP   Proximity Label-MS Homo sapiens 66 FGFBP1 9982 Affinity Capture-MS Homo sapiens 67 ABT1 29777 Affinity Capture-MS Homo sapiens 68 TCEAL7   Affinity Capture-MS Homo sapiens 69 PSENEN   Affinity Capture-MS Homo sapiens 70 SIRT7   Affinity Capture-MS Homo sapiens 71 HTATSF1 27336 Co-fractionation Homo sapiens 72 NR3C1 2908 Proximity Label-MS Homo sapiens 73 DNAJC17   Proximity Label-MS Homo sapiens 74 NXF1 10482 Affinity Capture-RNA Homo sapiens 75 MYCN   Affinity Capture-MS Homo sapiens 76 RPA3 6119 Proximity Label-MS Homo sapiens 77 LBH   Affinity Capture-MS Homo sapiens 78 PCBP1 5093 Affinity Capture-MS Homo sapiens 79 MYC   Affinity Capture-MS Homo sapiens 80 DDX47 51202 Affinity Capture-MS Homo sapiens 81 DNAJC9 23234 Proximity Label-MS Homo sapiens 82 ITPKB   Affinity Capture-MS Homo sapiens 83 RNF2   Affinity Capture-MS Homo sapiens 84 FAM107B 83641 Affinity Capture-MS Homo sapiens 85 SEPT7 989 Co-fractionation Homo sapiens 86 TMSB4Y   Affinity Capture-MS Homo sapiens 87 DDX52   Co-fractionation Homo sapiens 88 NGDN   Affinity Capture-MS Homo sapiens 89 HECTD1 25831 Affinity Capture-MS Homo sapiens 90 SIRT6   Affinity Capture-MS Homo sapiens 91 PARP1 142 Proximity Label-MS Homo sapiens 92 FBXL6   Affinity Capture-MS Homo sapiens 93 EHHADH 1962 Affinity Capture-MS Homo sapiens 94 2310003H01Rik   Affinity Capture-MS Mus musculus 95 IFI6   Affinity Capture-MS Homo sapiens 96 EZH1   Affinity Capture-MS Homo sapiens 97 NIFK 84365 Proximity Label-MS Homo sapiens 98 DHX40   Proximity Label-MS Homo sapiens 99 C20orf197   Affinity Capture-MS Homo sapiens 100 NUP50 10762 Proximity Label-MS Homo sapiens 101 RPS24 6229 Proximity Label-MS Homo sapiens 102 SPCS1 28972 Affinity Capture-MS Homo sapiens 103 CIT 11113 Affinity Capture-MS Homo sapiens 104 RNF145   Affinity Capture-MS Homo sapiens 105 KRAS 3845 Negative Genetic Homo sapiens 106 HNRNPU 3192 Affinity Capture-MS Homo sapiens 107 CYCS 54205 Affinity Capture-MS Homo sapiens 108 SMURF2 64750 Affinity Capture-MS Homo sapiens 109 Bcl7c   Affinity Capture-MS Mus musculus 110 UQCR11   Affinity Capture-MS Homo sapiens 111 ZBTB44   Affinity Capture-MS Homo sapiens View the network

Pathways in which ESF1 is involved

No pathways found