Gene description for COL8A1
Gene name collagen, type VIII, alpha 1
Gene symbol COL8A1
Other names/aliases C3orf7
Species Homo sapiens
 Database cross references - COL8A1
ExoCarta ExoCarta_1295
Entrez Gene 1295
HGNC 2215
MIM 120251
UniProt P27658  
 COL8A1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for COL8A1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    camera-type eye morphogenesis GO:0048593 IEA
    extracellular matrix organization GO:0030198 TAS
    extracellular matrix disassembly GO:0022617 TAS
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    angiogenesis GO:0001525 IEA
    cell adhesion GO:0007155 IEA
    epithelial cell proliferation GO:0050673 IEA
    endodermal cell differentiation GO:0035987 IEP
    collagen catabolic process GO:0030574 TAS
Subcellular Localization
    endoplasmic reticulum lumen GO:0005788 TAS
    extracellular exosome GO:0070062 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular matrix GO:0031012 IDA
    collagen type VIII trimer GO:0005591 TAS
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified COL8A1 in exosomes
Experiment ID 226
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for COL8A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCL 7414
Affinity Capture-MS Homo sapiens
2 COL8A2  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
3 KRTAP4-12  
Two-hybrid Homo sapiens
4 KLHL12  
Two-hybrid Homo sapiens
5 EFEMP2 30008
Two-hybrid Homo sapiens
View the network image/svg+xml

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