Gene description for COL8A1
Gene name collagen, type VIII, alpha 1
Gene symbol COL8A1
Other names/aliases C3orf7
Species Homo sapiens
 Database cross references - COL8A1
ExoCarta ExoCarta_1295
Vesiclepedia VP_1295
Entrez Gene 1295
HGNC 2215
MIM 120251
UniProt P27658  
 COL8A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for COL8A1
Molecular Function
    protein binding GO:0005515 IPI
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
Biological Process
    angiogenesis GO:0001525 IEA
    endothelial cell proliferation GO:0001935 IEA
    cell adhesion GO:0007155 IEA
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    extracellular matrix organization GO:0030198 IBA
    endodermal cell differentiation GO:0035987 IEP
    camera-type eye morphogenesis GO:0048593 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen type VIII trimer GO:0005591 TAS
    extracellular space GO:0005615 IBA
    endoplasmic reticulum lumen GO:0005788 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified COL8A1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for COL8A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBMXL1  
Affinity Capture-MS Homo sapiens
2 ARMC5 79798
Two-hybrid Homo sapiens
3 BPIFA1 51297
Two-hybrid Homo sapiens
4 KRTAP12-3  
Two-hybrid Homo sapiens
5 ZNHIT1  
Two-hybrid Homo sapiens
6 RBPMS 11030
Two-hybrid Homo sapiens
7 FRS3 10817
Two-hybrid Homo sapiens
8 REL 5966
Two-hybrid Homo sapiens
9 LIMS1 3987
Two-hybrid Homo sapiens
10 ESF1 51575
Affinity Capture-MS Homo sapiens
11 KRT37 8688
Two-hybrid Homo sapiens
12 KRT82 3888
Two-hybrid Homo sapiens
13 CWC25 54883
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 LSM14A 26065
Affinity Capture-MS Homo sapiens
16 TCEB3 6924
Affinity Capture-MS Homo sapiens
17 CENPU  
Affinity Capture-MS Homo sapiens
18 KRTAP19-7  
Two-hybrid Homo sapiens
19 KRTAP19-2  
Two-hybrid Homo sapiens
20 SSC4D  
Two-hybrid Homo sapiens
21 GTF2F2 2963
Affinity Capture-MS Homo sapiens
22 COL5A1 1289
Affinity Capture-MS Homo sapiens
23 MCM2 4171
Affinity Capture-MS Homo sapiens
24 TRIM35 23087
Two-hybrid Homo sapiens
25 KRT40  
Two-hybrid Homo sapiens
26 FBLN2 2199
Two-hybrid Homo sapiens
27 LSG1  
Affinity Capture-MS Homo sapiens
28 CIDEB  
Two-hybrid Homo sapiens
29 FAM207A  
Affinity Capture-MS Homo sapiens
30 FAM86C1  
Two-hybrid Homo sapiens
31 KPRP 448834
Two-hybrid Homo sapiens
32 P3H1 64175
Affinity Capture-MS Homo sapiens
33 KRTAP19-5  
Two-hybrid Homo sapiens
34 UBQLN4 56893
Two-hybrid Homo sapiens
35 COL2A1 1280
Affinity Capture-MS Homo sapiens
36 KRTAP5-6  
Two-hybrid Homo sapiens
37 C19orf54  
Two-hybrid Homo sapiens
38 BAG5 9529
Affinity Capture-MS Homo sapiens
39 POF1B 79983
Two-hybrid Homo sapiens
40 P4HA1 5033
Affinity Capture-MS Homo sapiens
41 CEP55 55165
Two-hybrid Homo sapiens
42 RGS20 8601
Two-hybrid Homo sapiens
43 NOV 4856
Two-hybrid Homo sapiens
44 KRTAP13-1  
Two-hybrid Homo sapiens
45 NFATC2IP  
Affinity Capture-MS Homo sapiens
46 IWS1  
Affinity Capture-MS Homo sapiens
47 KRTAP6-2  
Two-hybrid Homo sapiens
48 SPRY4 81848
Two-hybrid Homo sapiens
49 CYSRT1 375791
Two-hybrid Homo sapiens
50 C11orf1  
Two-hybrid Homo sapiens
51 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 COL8A2 1296
Reconstituted Complex Homo sapiens
53 BMP2K 55589
Affinity Capture-MS Homo sapiens
54 NOB1  
Affinity Capture-MS Homo sapiens
55 SNRNP27  
Affinity Capture-MS Homo sapiens
56 GTPBP4 23560
Affinity Capture-MS Homo sapiens
57 C22orf39  
Two-hybrid Homo sapiens
58 CCDC85C  
Affinity Capture-MS Homo sapiens
59 KRTAP10-7  
Two-hybrid Homo sapiens
60 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 HABP4  
Affinity Capture-MS Homo sapiens
62 ZAK 51776
Affinity Capture-MS Homo sapiens
63 KRTAP1-3  
Two-hybrid Homo sapiens
64 C9orf24  
Two-hybrid Homo sapiens
65 WFDC5  
Two-hybrid Homo sapiens
66 C11orf85  
Two-hybrid Homo sapiens
67 UBQLN2 29978
Two-hybrid Homo sapiens
68 KRTAP9-3  
Two-hybrid Homo sapiens
69 P4HA2 8974
Affinity Capture-MS Homo sapiens
70 DCAF13 25879
Affinity Capture-MS Homo sapiens
71 KRTAP10-3  
Two-hybrid Homo sapiens
72 HOXA1 3198
Two-hybrid Homo sapiens
73 SPATA8  
Two-hybrid Homo sapiens
74 KRTAP5-9  
Two-hybrid Homo sapiens
75 OTX1  
Two-hybrid Homo sapiens
76 RP9  
Affinity Capture-MS Homo sapiens
77 NADSYN1 55191
Two-hybrid Homo sapiens
78 TJP2 9414
Affinity Capture-MS Homo sapiens
79 PPARA  
Two-hybrid Homo sapiens
80 CRTAP 10491
Affinity Capture-MS Homo sapiens
81 HSPA1A 3303
Affinity Capture-MS Homo sapiens
82 COLGALT2  
Affinity Capture-MS Homo sapiens
83 KRTAP9-2  
Two-hybrid Homo sapiens
84 LOC100131303  
Two-hybrid Homo sapiens
85 COL4A1 1282
Affinity Capture-MS Homo sapiens
86 KRTAP3-2  
Two-hybrid Homo sapiens
87 KRTAP6-3  
Two-hybrid Homo sapiens
88 FBXO34  
Two-hybrid Homo sapiens
89 DDX46 9879
Affinity Capture-MS Homo sapiens
90 RRP36 88745
Affinity Capture-MS Homo sapiens
91 PRRC2A 7916
Affinity Capture-MS Homo sapiens
92 DIDO1  
Affinity Capture-MS Homo sapiens
93 SLC35A2  
Two-hybrid Homo sapiens
94 VAC14 55697
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
95 WBP11  
Affinity Capture-MS Homo sapiens
96 NME4 4833
Affinity Capture-MS Homo sapiens
97 MEN1 4221
Affinity Capture-MS Homo sapiens
98 NBPF19  
Two-hybrid Homo sapiens
99 KLHL12  
Two-hybrid Homo sapiens
100 ZC2HC1A 51101
Affinity Capture-MS Homo sapiens
101 COL18A1 80781
Affinity Capture-MS Homo sapiens
102 PLSCR3 57048
Two-hybrid Homo sapiens
103 KRTAP5-7  
Two-hybrid Homo sapiens
104 CREB5 9586
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
105 KRTAP4-12  
Two-hybrid Homo sapiens
106 SP4  
Two-hybrid Homo sapiens
107 SNIP1  
Affinity Capture-MS Homo sapiens
108 KRTAP26-1  
Two-hybrid Homo sapiens
109 REPS1 85021
Affinity Capture-MS Homo sapiens
110 POU4F2  
Two-hybrid Homo sapiens
111 GLRX3 10539
Two-hybrid Homo sapiens
112 ZC3H13 23091
Affinity Capture-MS Homo sapiens
113 KRTAP13-3  
Two-hybrid Homo sapiens
114 INCA1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
115 ZNF774  
Two-hybrid Homo sapiens
116 C1QL4  
Affinity Capture-MS Homo sapiens
117 EFEMP2 30008
Two-hybrid Homo sapiens
View the network image/svg+xml



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