Gene description for COL2A1
Gene name collagen, type II, alpha 1
Gene symbol COL2A1
Other names/aliases ANFH
AOM
COL11A3
SEDC
STL1
Species Homo sapiens
 Database cross references - COL2A1
ExoCarta ExoCarta_1280
Vesiclepedia VP_1280
Entrez Gene 1280
HGNC 2200
MIM 120140
UniProt P02458  
 COL2A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for COL2A1
Molecular Function
    metal ion binding GO:0046872 IEA
    identical protein binding GO:0042802 NAS
    platelet-derived growth factor binding GO:0048407 IDA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IC
Biological Process
    cartilage condensation GO:0001502 IEA
    collagen catabolic process GO:0030574 TAS
    tissue homeostasis GO:0001894 IEA
    central nervous system development GO:0007417 IEA
    limb bud formation GO:0060174 IEA
    regulation of gene expression GO:0010468 IEA
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IEA
    proteoglycan metabolic process GO:0006029 IEA
    endochondral ossification GO:0001958 IEA
    otic vesicle development GO:0071599 IEA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IEA
    palate development GO:0060021 IEA
    extracellular matrix disassembly GO:0022617 TAS
    inner ear morphogenesis GO:0042472 IEA
    skeletal system development GO:0001501 IMP
    embryonic skeletal joint morphogenesis GO:0060272 IMP
    visual perception GO:0007601 IMP
    cellular response to BMP stimulus GO:0071773 IEA
    cartilage development GO:0051216 TAS
    extracellular matrix organization GO:0030198 TAS
    sensory perception of sound GO:0007605 IMP
    heart morphogenesis GO:0003007 IEA
    notochord development GO:0030903 IEA
    axon guidance GO:0007411 TAS
    collagen fibril organization GO:0030199 IMP
    chondrocyte differentiation GO:0002062 IEA
Subcellular Localization
    basement membrane GO:0005604 IEA
    extracellular space GO:0005615 IEA
    extracellular region GO:0005576 TAS
    collagen type II trimer GO:0005585 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    extracellular matrix GO:0031012 IDA
 Experiment description of studies that identified COL2A1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for COL2A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPARC 6678
Affinity Capture-MS Homo sapiens
2 COL9A1  
Reconstituted Complex Homo sapiens
3 BMP2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 ITGA2B 3674
Reconstituted Complex Homo sapiens
5 BGN 633
Invitro Homo sapiens
6 DCN 1634
Affinity Capture-MS Homo sapiens
7 COL2A1 1280
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
8 C1QA 712
Invitro Homo sapiens
9 Igfbp7  
Affinity Capture-Western Mus musculus
10 LOXL4 84171
Affinity Capture-MS Homo sapiens
11 PKD1 5310
Invitro Homo sapiens
12 FN1 2335
Invivo Homo sapiens
13 MATN1  
Invivo Homo sapiens
14 MAG  
Invitro Homo sapiens
15 TGFBI 7045
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
16 COMP 1311
Invitro Homo sapiens
17 COL9A3  
Affinity Capture-MS Homo sapiens
18 TGFB1 7040
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
19 ANXA5 308
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
20 THBS1 7057
Reconstituted Complex Homo sapiens
21 FGF7  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
22 PRELP 5549
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
23 CHAD  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
24 TNFRSF10A 8797
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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