Gene description for MMP2
Gene name matrix metallopeptidase 2
Gene symbol MMP2
Other names/aliases CLG4
CLG4A
MMP-2
MMP-II
MONA
TBE-1
Species Homo sapiens
 Database cross references - MMP2
ExoCarta ExoCarta_4313
Vesiclepedia VP_4313
Entrez Gene 4313
HGNC 7166
MIM 120360
UniProt P08253  
 MMP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MMP2
Molecular Function
    fibronectin binding GO:0001968 IEA
    endopeptidase activity GO:0004175 IDA
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 TAS
    serine-type endopeptidase activity GO:0004252 EXP
    serine-type endopeptidase activity GO:0004252 TAS
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 IDA
    zinc ion binding GO:0008270 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    ovarian follicle development GO:0001541 IEA
    ovulation from ovarian follicle GO:0001542 IEA
    luteinization GO:0001553 IEA
    response to hypoxia GO:0001666 IBA
    blood vessel maturation GO:0001955 IEA
    intramembranous ossification GO:0001957 IEA
    proteolysis GO:0006508 IDA
    negative regulation of cell adhesion GO:0007162 IEA
    heart development GO:0007507 IEA
    embryo implantation GO:0007566 IEA
    parturition GO:0007567 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to mechanical stimulus GO:0009612 IEA
    peripheral nervous system axon regeneration GO:0014012 IEA
    response to activity GO:0014823 IEA
    cell migration GO:0016477 IMP
    extracellular matrix disassembly GO:0022617 TAS
    protein catabolic process GO:0030163 IEA
    extracellular matrix organization GO:0030198 IBA
    positive regulation of cell migration GO:0030335 IEA
    collagen catabolic process GO:0030574 IBA
    collagen catabolic process GO:0030574 TAS
    response to retinoic acid GO:0032526 IEA
    cellular response to reactive oxygen species GO:0034614 IEA
    response to nicotine GO:0035094 IEA
    endodermal cell differentiation GO:0035987 IEP
    response to hydrogen peroxide GO:0042542 IEA
    response to estrogen GO:0043627 IEA
    negative regulation of vasoconstriction GO:0045906 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    macrophage chemotaxis GO:0048246 IEA
    tissue remodeling GO:0048771 IBA
    response to electrical stimulus GO:0051602 IEA
    response to hyperoxia GO:0055093 IEA
    face morphogenesis GO:0060325 IEA
    bone trabecula formation GO:0060346 IEA
    prostate gland epithelium morphogenesis GO:0060740 IEA
    cellular response to amino acid stimulus GO:0071230 IEA
    cellular response to interleukin-1 GO:0071347 IEA
    cellular response to estradiol stimulus GO:0071392 IEA
    cellular response to UV-A GO:0071492 IDA
    cellular response to fluid shear stress GO:0071498 IEA
    positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903378 IEA
    response to amyloid-beta GO:1904645 ISS
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 IEA
    plasma membrane GO:0005886 IEA
    sarcomere GO:0030017 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified MMP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MMP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ITGB1 3688
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
3 COL6A1 1291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PZP 5858
Reconstituted Complex Homo sapiens
5 KLHL15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 OSBPL10  
Affinity Capture-MS Homo sapiens
7 BCAN  
Biochemical Activity Homo sapiens
8 COL4A2 1284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 COL4A1 1282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COLGALT2  
Affinity Capture-MS Homo sapiens
11 COL2A1 1280
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MTNR1B  
Two-hybrid Homo sapiens
13 BACE1  
Co-localization Homo sapiens
14 ITGB3 3690
Affinity Capture-Western Homo sapiens
15 COL4A6 1288
Affinity Capture-MS Homo sapiens
16 USP12 219333
Affinity Capture-MS Homo sapiens
17 SKP2  
Affinity Capture-MS Homo sapiens
18 MTNR1A  
Two-hybrid Homo sapiens
19 COL18A1 80781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CAND1 55832
Affinity Capture-MS Homo sapiens
21 TIMP3 7078
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 COL12A1 1303
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 A2M 2
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
24 FN1 2335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 THBS2 7058
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
26 COL6A2 1292
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 COL1A1 1277
Reconstituted Complex Homo sapiens
28 ITGAV 3685
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PLOD3 8985
Affinity Capture-MS Homo sapiens
31 TIMP4  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
32 TGFB1 7040
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
33 COL5A1 1289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CCL7 6354
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
35 THBS1 7057
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
36 COLEC12 81035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 LCN2 3934
Affinity Capture-Western Homo sapiens
38 RC3H1 149041
Affinity Capture-RNA Homo sapiens
39 TIMP2 7077
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 WISP2 8839
Biochemical Activity Homo sapiens
42 PAK4 10298
Affinity Capture-Western Homo sapiens
Synthetic Growth Defect Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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