Gene description for COL1A1
Gene name collagen, type I, alpha 1
Gene symbol COL1A1
Other names/aliases EDSC
OI1
OI2
OI3
OI4
Species Homo sapiens
 Database cross references - COL1A1
ExoCarta ExoCarta_1277
Entrez Gene 1277
HGNC 2197
MIM 120150
UniProt P02452  
 COL1A1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for COL1A1
Molecular Function
    extracellular matrix structural constituent GO:0005201 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    identical protein binding GO:0042802 IDA
    platelet-derived growth factor binding GO:0048407 IDA
Biological Process
    collagen biosynthetic process GO:0032964 IMP
    sensory perception of sound GO:0007605 IMP
    tooth mineralization GO:0034505 IMP
    cellular response to tumor necrosis factor GO:0071356 IEA
    positive regulation of cell migration GO:0030335 IDA
    cellular response to vitamin E GO:0071306 IEA
    bone trabecula formation GO:0060346 IEA
    response to hydrogen peroxide GO:0042542 IEA
    intramembranous ossification GO:0001957 IEA
    negative regulation of cell-substrate adhesion GO:0010812 IEA
    osteoblast differentiation GO:0001649 IEA
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IEA
    skin morphogenesis GO:0043589 IMP
    response to hyperoxia GO:0055093 IEA
    collagen fibril organization GO:0030199 IMP
    endochondral ossification GO:0001958 IEA
    cellular response to retinoic acid GO:0071300 IEA
    face morphogenesis GO:0060325 IEA
    collagen catabolic process GO:0030574 TAS
    leukocyte migration GO:0050900 TAS
    extracellular matrix disassembly GO:0022617 TAS
    skeletal system development GO:0001501 IMP
    tooth eruption GO:0044691 IEA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IDA
    blood vessel development GO:0001568 IMP
    response to peptide hormone GO:0043434 IEA
    protein localization to nucleus GO:0034504 IDA
    cellular response to fluoride GO:1902618 IEA
    protein heterotrimerization GO:0070208 IEA
    cellular response to mechanical stimulus GO:0071260 IEA
    protein transport GO:0015031 IEA
    response to cAMP GO:0051591 IEA
    blood coagulation GO:0007596 TAS
    cellular response to amino acid stimulus GO:0071230 IEA
    response to corticosteroid GO:0031960 IEA
    visual perception GO:0007601 IMP
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    response to drug GO:0042493 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
    cellular response to fibroblast growth factor stimulus GO:0044344 IEA
    embryonic skeletal system development GO:0048706 IMP
    response to estradiol GO:0032355 IEA
    receptor-mediated endocytosis GO:0006898 TAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    extracellular matrix organization GO:0030198 TAS
    platelet activation GO:0030168 TAS
Subcellular Localization
    extracellular matrix GO:0031012 ISS
    Golgi apparatus GO:0005794 IEA
    extracellular space GO:0005615 IDA
    extracellular region GO:0005576 TAS
    collagen type I trimer GO:0005584 IMP
    endoplasmic reticulum lumen GO:0005788 TAS
    secretory granule GO:0030141 IEA
 Experiment description of studies that identified COL1A1 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
10
Experiment ID 136
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
11
Experiment ID 137
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB6
EV Cytosolic markers
✔
CD10
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
12
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
13
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COL1A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Co-purification Homo sapiens
2 MMP2 4313
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 SPARC 6678
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 ITGA5 3678
Affinity Capture-MS Homo sapiens
5 BGN 633
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 CD36 948
Invitro Homo sapiens
7 MMP9 4318
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 DDR2 4921
Invitro Homo sapiens
9 TMPRSS6  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 IGFBP3  
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
11 NID2 22795
Reconstituted Complex Homo sapiens
12 PKD1 5310
Reconstituted Complex Homo sapiens
13 FN1 2335
Invivo Homo sapiens
14 DCN 1634
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
15 ITGA2 3673
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
16 COL7A1 1294
Reconstituted Complex Homo sapiens
17 TGFBI 7045
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
18 TXN 7295
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
19 C1QR1  
Affinity Capture-MS Homo sapiens
20 NID1 4811
Reconstituted Complex Homo sapiens
21 THBS1 7057
Reconstituted Complex Homo sapiens
22 P4HB 5034
Affinity Capture-MS Homo sapiens
23 PAK1  
Invitro Homo sapiens
24 CD44 960
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
25 Nid1 18073
Reconstituted Complex Mus musculus
26 FGF7  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
27 PRELP 5549
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
28 MATN2 4147
Reconstituted Complex Homo sapiens
29 PDGFB  
Affinity Capture-MS Homo sapiens
30 VWF 7450
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here