Gene description for VWF
Gene name von Willebrand factor
Gene symbol VWF
Other names/aliases F8VWF
VWD
Species Homo sapiens
 Database cross references - VWF
ExoCarta ExoCarta_7450
Vesiclepedia VP_7450
Entrez Gene 7450
HGNC 12726
MIM 613160
UniProt P04275  
 VWF identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for VWF
Molecular Function
    immunoglobulin binding GO:0019865 IDA
    collagen binding GO:0005518 IDA
    protein homodimerization activity GO:0042803 IDA
    integrin binding GO:0005178 IPI
    protease binding GO:0002020 IPI
    protein N-terminus binding GO:0047485 IPI
    glycoprotein binding GO:0001948 IDA
    protein binding GO:0005515 IPI
    chaperone binding GO:0051087 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    hemostasis GO:0007599 IMP
    platelet degranulation GO:0002576 TAS
    blood coagulation GO:0007596 TAS
    extracellular matrix organization GO:0030198 TAS
    response to wounding GO:0009611 TAS
    cell-substrate adhesion GO:0031589 IDA
    protein homooligomerization GO:0051260 IDA
    cell adhesion GO:0007155 IDA
    blood coagulation, intrinsic pathway GO:0007597 TAS
    platelet activation GO:0030168 TAS
    placenta development GO:0001890 IEA
    liver development GO:0001889 IEA
Subcellular Localization
    Weibel-Palade body GO:0033093 IDA
    proteinaceous extracellular matrix GO:0005578 IEA
    extracellular exosome GO:0070062 IDA
    platelet alpha granule GO:0031091 NAS
    extracellular matrix GO:0031012 IDA
    platelet alpha granule lumen GO:0031093 TAS
    extracellular region GO:0005576 TAS
    external side of plasma membrane GO:0009897 IEA
    endoplasmic reticulum GO:0005783 IDA
 Experiment description of studies that identified VWF in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 44
MISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for VWF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL1A1 1277
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
2 CALR 811
Affinity Capture-Western Homo sapiens
3 HSPA5 3309
Affinity Capture-Western Homo sapiens
4 ITGA2B 3674
Invivo Homo sapiens
Invitro Homo sapiens
5 F8 2157
Reconstituted Complex Homo sapiens
6 GP1BA 2811
Co-crystal Structure Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
7 HSP90B1 7184
Affinity Capture-Western Homo sapiens
8 PDIA4 9601
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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