Gene description for LAT
Gene name linker for activation of T cells
Gene symbol LAT
Other names/aliases LAT1
pp36
Species Homo sapiens
 Database cross references - LAT
ExoCarta ExoCarta_27040
Entrez Gene 27040
HGNC 18874
MIM 602354
UniProt O43561  
 LAT identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for LAT
Molecular Function
    SH3/SH2 adaptor activity GO:0005070 IDA
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
Biological Process
    Ras protein signal transduction GO:0007265 IMP
    T cell receptor signaling pathway GO:0050852 TAS
    calcium-mediated signaling GO:0019722 IMP
    positive regulation of signal transduction GO:0009967 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    regulation of T cell activation GO:0050863 IMP
    positive regulation of protein kinase activity GO:0045860 IEP
    platelet activation GO:0030168 TAS
    blood coagulation GO:0007596 TAS
    innate immune response GO:0045087 TAS
    gene expression GO:0010467 IEA
    intracellular signal transduction GO:0035556 IDA
    mast cell degranulation GO:0043303 IEA
    lymphocyte homeostasis GO:0002260 IEA
    inflammatory response GO:0006954 IEA
    T cell activation GO:0042110 TAS
    immune response GO:0006955 IDA
    integrin-mediated signaling pathway GO:0007229 IDA
Subcellular Localization
    mast cell granule GO:0042629 IEA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IEA
    immunological synapse GO:0001772 IDA
    membrane raft GO:0045121 TAS
    integral component of membrane GO:0016021 IDA
    COP9 signalosome GO:0008180 ISS
 Experiment description of studies that identified LAT in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 PTPRJ 5795
Invitro Homo sapiens
3 CD4 920
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
4 FCGR1A  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
5 CD8A 925
Invivo Homo sapiens
6 SYK 6850
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
7 CLYBL  
Affinity Capture-Western Homo sapiens
8 PLCG1 5335
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 GAB2 9846
Affinity Capture-Western Homo sapiens
10 PLCG2 5336
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
11 SH3BP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 VAV1 7409
Reconstituted Complex Homo sapiens
13 GRB2 2885
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
14 FCGR2A 2212
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
15 ITK 3702
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
16 SLA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 GRAP2 9402
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
18 LCP2 3937
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
19 SHB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 ZAP70 7535
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which LAT is involved
PathwayEvidenceSource
Generation of second messenger molecules TAS Reactome
GPVI-mediated activation cascade TAS Reactome





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