Gene description for GJB1
Gene name gap junction protein, beta 1, 32kDa
Gene symbol GJB1
Other names/aliases CMTX
CMTX1
CX32
Species Homo sapiens
 Database cross references - GJB1
ExoCarta ExoCarta_2705
Vesiclepedia VP_2705
Entrez Gene 2705
HGNC 4283
MIM 304040
UniProt P08034  
 GJB1 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for GJB1
Molecular Function
    protein homodimerization activity GO:0042803 IEA
Biological Process
    cell-cell signaling GO:0007267 TAS
    nervous system development GO:0007399 TAS
    membrane organization GO:0061024 TAS
    transport GO:0006810 TAS
    gap junction assembly GO:0016264 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    connexon complex GO:0005922 IEA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified GJB1 in exosomes
1
Experiment ID 255
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 256
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 257
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GJB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OCLN  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 GJB2  
Affinity Capture-MS Homo sapiens
3 JUP 3728
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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