Gene description for VEGFA
Gene name vascular endothelial growth factor A
Gene symbol VEGFA
Other names/aliases MVCD1
VEGF
VPF
Species Homo sapiens
 Database cross references - VEGFA
ExoCarta ExoCarta_7422
Vesiclepedia VP_7422
Entrez Gene 7422
HGNC 12680
MIM 192240
UniProt P15692  
 VEGFA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for VEGFA
Molecular Function
    fibronectin binding GO:0001968 IDA
    cytokine activity GO:0005125 IDA
    cytokine activity GO:0005125 ISS
    platelet-derived growth factor receptor binding GO:0005161 IPI
    vascular endothelial growth factor receptor binding GO:0005172 IBA
    vascular endothelial growth factor receptor binding GO:0005172 IPI
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IBA
    growth factor activity GO:0008083 IDA
    growth factor activity GO:0008083 ISS
    heparin binding GO:0008201 IDA
    heparin binding GO:0008201 IMP
    transmembrane receptor protein tyrosine kinase activator activity GO:0030297 IDA
    neuropilin binding GO:0038191 IPI
    chemoattractant activity GO:0042056 IBA
    chemoattractant activity GO:0042056 IDA
    chemoattractant activity GO:0042056 ISS
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 ISS
    vascular endothelial growth factor receptor 1 binding GO:0043183 IPI
    vascular endothelial growth factor receptor 2 binding GO:0043184 IPI
    receptor ligand activity GO:0048018 IMP
    receptor ligand activity GO:0048018 IPI
    extracellular matrix binding GO:0050840 IC
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    angiogenesis GO:0001525 IDA
    ovarian follicle development GO:0001541 ISS
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    vasculogenesis GO:0001570 TAS
    response to hypoxia GO:0001666 IBA
    response to hypoxia GO:0001666 IDA
    in utero embryonic development GO:0001701 ISS
    kidney development GO:0001822 ISS
    positive regulation of protein phosphorylation GO:0001934 IDA
    endothelial cell proliferation GO:0001935 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IDA
    positive regulation of endothelial cell proliferation GO:0001938 IDA
    positive regulation of endothelial cell proliferation GO:0001938 ISS
    lymphangiogenesis GO:0001946 IEA
    sprouting angiogenesis GO:0002040 IBA
    cell migration involved in sprouting angiogenesis GO:0002042 IDA
    positive regulation of neuroblast proliferation GO:0002052 ISS
    epithelial cell maturation GO:0002070 IEA
    positive regulation of receptor internalization GO:0002092 IDA
    basophil chemotaxis GO:0002575 IDA
    positive regulation of leukocyte migration GO:0002687 TAS
    heart morphogenesis GO:0003007 ISS
    outflow tract morphogenesis GO:0003151 ISS
    coronary vein morphogenesis GO:0003169 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    apoptotic process GO:0006915 IEA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IDA
    nervous system development GO:0007399 TAS
    neuroblast proliferation GO:0007405 IEA
    mesoderm development GO:0007498 ISS
    lactation GO:0007595 ISS
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IMP
    regulation of cell shape GO:0008360 IDA
    positive regulation of endothelial cell migration GO:0010595 IDA
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IDA
    negative regulation of gene expression GO:0010629 IMP
    negative regulation of epithelial to mesenchymal transition GO:0010719 IEA
    regulation of nitric oxide mediated signal transduction GO:0010749 IDA
    monocyte differentiation GO:0030224 IDA
    macrophage differentiation GO:0030225 IDA
    lung development GO:0030324 ISS
    positive regulation of cell migration GO:0030335 IDA
    epithelial cell differentiation GO:0030855 ISS
    post-embryonic camera-type eye development GO:0031077 ISS
    retinal ganglion cell axon guidance GO:0031290 ISS
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    positive regulation of protein autophosphorylation GO:0031954 IDA
    tube formation GO:0035148 IDA
    endothelial cell chemotaxis GO:0035767 IDA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IDA
    lymph vessel morphogenesis GO:0036303 ISS
    vascular endothelial growth factor receptor-2 signaling pathway GO:0036324 IMP
    positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway GO:0038033 IDA
    vascular endothelial growth factor signaling pathway GO:0038084 IBA
    vascular endothelial growth factor signaling pathway GO:0038084 IDA
    vascular endothelial growth factor signaling pathway GO:0038084 IMP
    vascular endothelial growth factor signaling pathway GO:0038084 NAS
    positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway GO:0038091 IDA
    VEGF-activated neuropilin signaling pathway GO:0038190 IMP
    VEGF-activated neuropilin signaling pathway GO:0038190 ISS
    vasodilation GO:0042311 IEA
    eye photoreceptor cell development GO:0042462 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    positive regulation of vascular permeability GO:0043117 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 NAS
    surfactant homeostasis GO:0043129 ISS
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    negative regulation of fat cell differentiation GO:0045599 IEA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of angiogenesis GO:0045766 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of cell adhesion GO:0045785 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IBA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IDA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    cell maturation GO:0048469 ISS
    camera-type eye morphogenesis GO:0048593 ISS
    positive regulation of axon extension involved in axon guidance GO:0048842 ISS
    artery morphogenesis GO:0048844 ISS
    homeostasis of number of cells within a tissue GO:0048873 IEA
    positive regulation of epithelial cell proliferation GO:0050679 ISS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    positive chemotaxis GO:0050918 IDA
    positive chemotaxis GO:0050918 IEA
    positive chemotaxis GO:0050918 ISS
    positive regulation of positive chemotaxis GO:0050927 IDA
    induction of positive chemotaxis GO:0050930 IBA
    induction of positive chemotaxis GO:0050930 IDA
    induction of positive chemotaxis GO:0050930 NAS
    positive regulation of cell division GO:0051781 IEA
    positive regulation of focal adhesion assembly GO:0051894 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IGI
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    cardiac muscle cell development GO:0055013 ISS
    primitive erythrocyte differentiation GO:0060319 ISS
    bone trabecula formation GO:0060346 IEA
    lung vasculature development GO:0060426 IEA
    mammary gland alveolus development GO:0060749 ISS
    positive regulation of mast cell chemotaxis GO:0060754 IBA
    positive regulation of mast cell chemotaxis GO:0060754 IDA
    cardiac vascular smooth muscle cell development GO:0060948 ISS
    coronary artery morphogenesis GO:0060982 ISS
    vascular wound healing GO:0061042 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    cellular response to hypoxia GO:0071456 IDA
    cellular response to hypoxia GO:0071456 IMP
    dopaminergic neuron differentiation GO:0071542 ISS
    commissural neuron axon guidance GO:0071679 ISS
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IDA
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IGI
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IMP
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 ISS
    motor neuron migration GO:0097475 ISS
    cellular stress response to acid chemical GO:0097533 IDA
    cell-cell adhesion GO:0098609 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    positive regulation of p38MAPK cascade GO:1900745 IDA
    positive regulation of vascular endothelial growth factor signaling pathway GO:1900748 IDA
    positive regulation of trophoblast cell migration GO:1901165 IMP
    positive regulation of lymphangiogenesis GO:1901492 IEA
    regulation of hematopoietic progenitor cell differentiation GO:1901532 IEA
    negative regulation of miRNA transcription GO:1902894 NAS
    positive regulation of protein localization to early endosome GO:1902966 IDA
    negative regulation of establishment of endothelial barrier GO:1903141 IDA
    negative regulation of adherens junction organization GO:1903392 IDA
    positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:1903589 IGI
    positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:1903589 IMP
    positive regulation of sprouting angiogenesis GO:1903672 IDA
    positive regulation of sprouting angiogenesis GO:1903672 IGI
    positive regulation of epithelial tube formation GO:1905278 IDA
    negative regulation of blood-brain barrier permeability GO:1905604 IMP
    negative regulation of cell-cell adhesion mediated by cadherin GO:2000048 IDA
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IDA
    positive regulation of DNA biosynthetic process GO:2000573 IDA
    positive regulation of DNA biosynthetic process GO:2000573 IGI
    positive regulation of endothelial cell chemotaxis GO:2001028 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IEA
    adherens junction GO:0005912 IDA
    cell surface GO:0009986 IDA
    membrane GO:0016020 IEA
    secretory granule GO:0030141 IDA
    extracellular matrix GO:0031012 NAS
    platelet alpha granule lumen GO:0031093 TAS
    VEGF-A complex GO:1990150 IPI
 Experiment description of studies that identified VEGFA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for VEGFA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 VPS35 55737
Two-hybrid Homo sapiens
3 PRRG4  
Two-hybrid Homo sapiens
4 HNRNPD 3184
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
Protein-RNA Homo sapiens
5 NRP1 8829
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 ILF3 3609
Affinity Capture-RNA Homo sapiens
7 SLC16A2  
Two-hybrid Homo sapiens
8 VTN 7448
Reconstituted Complex Homo sapiens
9 CRYAB 1410
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
10 GPC1 2817
Reconstituted Complex Homo sapiens
11 MEX3A  
Affinity Capture-RNA Homo sapiens
12 PGF  
Co-purification Homo sapiens
Co-localization Homo sapiens
13 BRCA1 672
Negative Genetic Homo sapiens
14 TMEM237 65062
Two-hybrid Homo sapiens
15 ACTBL2 345651
Affinity Capture-MS Homo sapiens
16 VEGFA 7422
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-crystal Structure Homo sapiens
17 Flt4  
Affinity Capture-Western Mus musculus
18 ELAVL1 1994
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
19 CHEK1  
Negative Genetic Homo sapiens
20 CLU 1191
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 NRP2 8828
Reconstituted Complex Homo sapiens
22 DDX58 23586
Affinity Capture-RNA Homo sapiens
23 CTGF 1490
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 VHL  
Negative Genetic Homo sapiens
25 SPARC 6678
Far Western Homo sapiens
26 IGFBP7 3490
Reconstituted Complex Homo sapiens
27 APEX1 328
Affinity Capture-RNA Homo sapiens
28 FLT1 2321
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 HNRNPL 3191
Affinity Capture-RNA Homo sapiens
30 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
31 PTPRZ1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
32 VEGFB  
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PTPRB 5787
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
34 HSPA4 3308
Affinity Capture-RNA Homo sapiens
Affinity Capture-Western Homo sapiens
35 GJB1 2705
Two-hybrid Homo sapiens
36 HGS 9146
Affinity Capture-Western Homo sapiens
37 STAT3 6774
Co-localization Homo sapiens
38 ADAMTS1 9510
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 LYVE1  
Reconstituted Complex Bos taurus
40 KDR  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
41 UBIAD1 29914
Two-hybrid Homo sapiens
42 USF2 7392
Co-localization Homo sapiens
43 LYVE1  
Reconstituted Complex Homo sapiens
44 HIF1A 3091
Co-localization Homo sapiens
45 U2AF1 7307
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which VEGFA is involved
PathwayEvidenceSource
Cellular response to hypoxia TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Interleukin-4 and Interleukin-13 signaling TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet degranulation TAS Reactome
Potential therapeutics for SARS TAS Reactome
Regulation of gene expression by Hypoxia-inducible Factor TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
RNA Polymerase II Transcription TAS Reactome
SARS-CoV Infections TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Interleukins TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by VEGF TAS Reactome
Signaling by VEGF IEA Reactome
TFAP2 (AP-2) family regulates transcription of growth factors and their receptors TAS Reactome
Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors TAS Reactome
VEGF binds to VEGFR leading to receptor dimerization TAS Reactome
VEGF ligand-receptor interactions TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated cell proliferation TAS Reactome
Viral Infection Pathways TAS Reactome





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