Gene description for USF2
Gene name upstream transcription factor 2, c-fos interacting
Gene symbol USF2
Other names/aliases FIP
bHLHb12
Species Homo sapiens
 Database cross references - USF2
ExoCarta ExoCarta_7392
Vesiclepedia VP_7392
Entrez Gene 7392
HGNC 12594
MIM 600390
UniProt Q15853  
 USF2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for USF2
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IMP
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
    bHLH transcription factor binding GO:0043425 IPI
    sequence-specific DNA binding GO:0043565 IDA
    protein heterodimerization activity GO:0046982 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    regulation of transcription from RNA polymerase II promoter by glucose GO:0000430 IC
    positive regulation of transcription from RNA polymerase II promoter by glucose GO:0000432 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    lactation GO:0007595 IEA
    glucose mediated signaling pathway GO:0010255 IMP
    late viral transcription GO:0019086 IC
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    lipid homeostasis GO:0055088 ISS
    cellular response to glucose stimulus GO:0071333 IMP
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified USF2 in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for USF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CALML3 810
Affinity Capture-MS Homo sapiens
2 RELA 5970
Two-hybrid Homo sapiens
3 SERPINB3 6317
Affinity Capture-MS Homo sapiens
4 PSG1  
Affinity Capture-MS Homo sapiens
5 WDR3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 VEGFA 7422
Co-localization Homo sapiens
7 FOXJ2  
Affinity Capture-MS Homo sapiens
8 BRCA1 672
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 TFAP4 7023
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
11 SERPINE1 5054
Co-localization Homo sapiens
12 PHAX  
Affinity Capture-MS Homo sapiens
13 S100A9 6280
Affinity Capture-MS Homo sapiens
14 CCDC102B  
Affinity Capture-MS Homo sapiens
15 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 EPAS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 LZTS2 84445
Two-hybrid Homo sapiens
19 CEBPA  
Proximity Label-MS Homo sapiens
20 ING1  
Affinity Capture-MS Homo sapiens
21 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FOS 2353
Two-hybrid Homo sapiens
23 SREBF2 6721
Positive Genetic Homo sapiens
24 FUS 2521
Affinity Capture-MS Homo sapiens
25 PTP4A2 8073
Affinity Capture-MS Homo sapiens
26 SERPINB4 6318
Affinity Capture-MS Homo sapiens
27 S100A8 6279
Affinity Capture-MS Homo sapiens
28 TEAD2  
Two-hybrid Homo sapiens
29 EPO 2056
Co-localization Homo sapiens
30 MAF  
Reconstituted Complex Homo sapiens
31 HSPA4L 22824
Affinity Capture-MS Homo sapiens
32 NPM1 4869
Affinity Capture-MS Homo sapiens
33 USF1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 FHL2 2274
Affinity Capture-MS Homo sapiens
35 USF2 7392
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 EP300 2033
Two-hybrid Homo sapiens
37 BIRC5  
Affinity Capture-MS Homo sapiens
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