Gene description for BRCA1
Gene name breast cancer 1, early onset
Gene symbol BRCA1
Other names/aliases BRCAI
BRCC1
BROVCA1
FANCS
IRIS
PNCA4
PPP1R53
PSCP
RNF53
Species Homo sapiens
 Database cross references - BRCA1
ExoCarta ExoCarta_672
Entrez Gene 672
HGNC 1100
MIM 113705
UniProt P38398  
 BRCA1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for BRCA1
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IPI
    protein binding GO:0005515 IPI
    RNA binding GO:0003723 IDA
    DNA binding GO:0003677 TAS
    ubiquitin-protein transferase activity GO:0004842 IDA
    transcription coactivator activity GO:0003713 NAS
    transcription regulatory region DNA binding GO:0044212 IDA
    zinc ion binding GO:0008270 IEA
    damaged DNA binding GO:0003684 IEA
    tubulin binding GO:0015631 NAS
    enzyme binding GO:0019899 IPI
    androgen receptor binding GO:0050681 NAS
    ligase activity GO:0016874 IEA
Biological Process
    negative regulation of fatty acid biosynthetic process GO:0045717 IMP
    regulation of DNA methylation GO:0044030 IEA
    positive regulation of histone H3-K9 acetylation GO:2000617 IDA
    regulation of cell proliferation GO:0042127 TAS
    androgen receptor signaling pathway GO:0030521 NAS
    positive regulation of transcription, DNA-templated GO:0045893 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    protein sumoylation GO:0016925 TAS
    negative regulation of reactive oxygen species metabolic process GO:2000378 IMP
    regulation of transcription from RNA polymerase III promoter GO:0006359 TAS
    protein ubiquitination GO:0016567 IDA
    protein autoubiquitination GO:0051865 IDA
    positive regulation of cell cycle arrest GO:0071158 IDA
    response to ionizing radiation GO:0010212 IMP
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IDA
    DNA repair GO:0006281 TAS
    centrosome cycle GO:0007098 IEA
    regulation of gene expression by genetic imprinting GO:0006349 IEA
    double-strand break repair via homologous recombination GO:0000724 TAS
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
    negative regulation of centriole replication GO:0046600 NAS
    positive regulation of histone H3-K4 methylation GO:0051571 IDA
    cellular response to tumor necrosis factor GO:0071356 IMP
    protein K6-linked ubiquitination GO:0085020 IDA
    cellular response to indole-3-methanol GO:0071681 IDA
    cellular response to DNA damage stimulus GO:0006974 TAS
    positive regulation of histone acetylation GO:0035066 IDA
    negative regulation of histone acetylation GO:0035067 IBA
    chordate embryonic development GO:0043009 IBA
    positive regulation of protein ubiquitination GO:0031398 IDA
    double-strand break repair GO:0006302 TAS
    chromosome segregation GO:0007059 IMP
    dosage compensation by inactivation of X chromosome GO:0009048 IBA
    apoptotic process GO:0006915 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    G2 DNA damage checkpoint GO:0031572 IMP
    positive regulation of histone H4-K20 methylation GO:0070512 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of histone H4-K16 acetylation GO:2000620 IDA
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of gene expression GO:0010628 IMP
    postreplication repair GO:0006301 IDA
    DNA replication GO:0006260 IEA
    negative regulation of histone H3-K9 methylation GO:0051573 IDA
    negative regulation of histone H3-K4 methylation GO:0051572 IEA
    DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0006978 TAS
    positive regulation of vascular endothelial growth factor production GO:0010575 IMP
    post-translational protein modification GO:0043687 TAS
    positive regulation of DNA repair GO:0045739 IMP
    positive regulation of histone H3-K9 methylation GO:0051574 IEA
    response to estrogen GO:0043627 IDA
    fatty acid biosynthetic process GO:0006633 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
Subcellular Localization
    BRCA1-BARD1 complex GO:0031436 IDA
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
    gamma-tubulin ring complex GO:0008274 NAS
    chromosome GO:0005694 ISS
    nucleoplasm GO:0005654 TAS
    protein complex GO:0043234 IDA
    nucleus GO:0005634 IDA
    BRCA1-A complex GO:0070531 IDA
    ubiquitin ligase complex GO:0000151 NAS
    ribonucleoprotein complex GO:0030529 IDA
    condensed nuclear chromosome GO:0000794 IEA
 Experiment description of studies that identified BRCA1 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for BRCA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 GTF2F1 2962
Co-purification Homo sapiens
3 NUP2  
Synthetic Rescue 4932
4 XIST  
Co-localization Homo sapiens
Co-localization Homo sapiens
5 CRSP6  
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
6 GTF2E1  
Co-purification Homo sapiens
7 MSH6 2956
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
8 MAP3K3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 STAT5A 6776
Affinity Capture-Western Homo sapiens
10 SP1  
Affinity Capture-Western Homo sapiens
11 CCR4  
Synthetic Rescue 4932
12 E2F1 1869
Invitro Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
13 ABL1 25
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 BRCA1 672
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
15 ASM4  
Synthetic Rescue 4932
16 JUND 3727
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 UBE3A 7337
Reconstituted Complex Homo sapiens
18 MAP4K4 9448
Protein-peptide Homo sapiens
19 CSNK2A1 1457
Reconstituted Complex Homo sapiens
20 CHEK2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 KPNA2 3838
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
22 POU2F1  
Affinity Capture-Western Homo sapiens
23 SMARCD2 6603
Co-purification Homo sapiens
24 MSH3 4437
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
25 PRKDC 5591
Protein-peptide Homo sapiens
26 ZNF350  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
27 DHH1  
Synthetic Rescue 4932
28 HDAC1 3065
Reconstituted Complex Homo sapiens
29 MED13 9969
Affinity Capture-MS Homo sapiens
30 TP53  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
31 NUFIP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 HDAC2 3066
Reconstituted Complex Homo sapiens
33 NUP153  
Protein-peptide Homo sapiens
34 NPM1 4869
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 NCOA3  
Protein-peptide Homo sapiens
36 RAD51  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
37 USF2 7392
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 HIST2H2AC 8338
Reconstituted Complex Homo sapiens
39 FHL2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 SMARCB1 6598
Co-purification Homo sapiens
Co-purification Homo sapiens
41 LMO4  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
42 HSPA8 3312
Two-hybrid Homo sapiens
43 POLR2K  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
44 NUP188  
Synthetic Rescue 4932
45 RB1 5925
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
46 E2F4  
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 PPP1CA 5499
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
48 CDK16 5127
Two-hybrid Homo sapiens
49 RBBP7 5931
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
50 BRIP1  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
51 MRE11A 4361
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
52 CTBP1 1487
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
53 BRE 9577
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
54 BLM 641
Co-purification Homo sapiens
55 NUP170  
Synthetic Rescue 4932
56 BACH1 571
Invivo Homo sapiens
Invitro Homo sapiens
57 WNT2B  
Far Western Homo sapiens
58 APLP2 334
Far Western Homo sapiens
59 ACACA 31
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
60 BRCA2 675
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
61 CCNB1 891
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
62 SMARCA4 6597
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
63 ATM  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
64 BARD1 580
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
65 RPL31 6160
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
66 PEG3 5178
Far Western Homo sapiens
67 UBE2D1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
68 SPT5  
Synthetic Rescue 4932
69 NUP133  
Synthetic Rescue 4932
70 DHX9 1660
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
71 BRAP  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
72 CSTF2  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
73 H2AFY 9555
Co-localization Homo sapiens
74 PPARBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
75 VCP 7415
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
76 POLR2B 5431
Reconstituted Complex Homo sapiens
77 RBL1  
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
78 FLI1  
Reconstituted Complex Homo sapiens
79 KIF1B 23095
Protein-peptide Homo sapiens
80 CCNA2 890
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
81 CSTF1 1477
Reconstituted Complex Homo sapiens
82 FANCD2  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
83 MSH2 4436
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
84 HIST2H3C 126961
Co-localization Homo sapiens
85 ELK4 2005
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
86 SMARCC2 6601
Co-purification Homo sapiens
87 UBE2L3 7332
Reconstituted Complex Homo sapiens
88 RELA  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
89 NBS1  
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-localization Homo sapiens
90 BAP1 8314
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
91 POLR2A 5430
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
92 RBBP4 5928
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
93 CLSPN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 RFC1 5981
Co-purification Homo sapiens
95 JUN 3725
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
96 ELK1  
Invivo Homo sapiens
Invitro Homo sapiens
97 CDK2 1017
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
98 FANCA  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
99 MLP2  
Synthetic Rescue 4932
100 GTF2H4  
Co-purification Homo sapiens
101 CTCFL  
Protein-peptide Homo sapiens
102 STAT1 6772
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
103 MYC  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
104 POM34  
Synthetic Rescue 4932
105 JAK1 3716
Affinity Capture-Western Homo sapiens
106 MRPL39  
Synthetic Rescue 4932
107 CXorf53  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
108 RBL2 5934
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
109 ETS1  
Reconstituted Complex Homo sapiens
110 CSNK2B 1460
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
111 JUNB  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
112 MED23 9439
Affinity Capture-MS Homo sapiens
113 NFYA 4800
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
114 AKT1 207
Reconstituted Complex Homo sapiens
115 CCND1 595
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
116 NELFB 25920
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
117 STK6  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
118 RBBP8  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
119 H2AFX 3014
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
120 DEF1