Gene description for NRIP1
Gene name nuclear receptor interacting protein 1
Gene symbol NRIP1
Other names/aliases RIP140
Species Homo sapiens
 Database cross references - NRIP1
ExoCarta ExoCarta_8204
Entrez Gene 8204
HGNC 8001
MIM 602490
UniProt P48552  
 NRIP1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NRIP1
Molecular Function
    transcription corepressor activity GO:0003714 IDA
    transcription coactivator activity GO:0003713 NAS
    estrogen receptor binding GO:0030331 IPI
    glucocorticoid receptor binding GO:0035259 IPI
    histone deacetylase binding GO:0042826 IEA
    nuclear hormone receptor binding GO:0035257 IPI
    androgen receptor binding GO:0050681 NAS
    core promoter sequence-specific DNA binding GO:0001046 IDA
    retinoid X receptor binding GO:0046965 IEA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    circadian regulation of gene expression GO:0032922 IMP
    transcription, DNA-templated GO:0006351 IEA
    lipid storage GO:0019915 IEA
    positive regulation of transcription, DNA-templated GO:0045893 NAS
    circadian rhythm GO:0007623 TAS
    ovarian follicle rupture GO:0001543 IEA
    cellular response to estradiol stimulus GO:0071392 IDA
    androgen receptor signaling pathway GO:0030521 NAS
Subcellular Localization
    histone deacetylase complex GO:0000118 IEA
    nuclear speck GO:0016607 IEA
    cell junction GO:0030054 IDA
    nuclear chromatin GO:0000790 IDA
    nucleolus GO:0005730 IDA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified NRIP1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NRIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NR2C1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 NR0B1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
3 RARA 5914
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 ESR1  
Reconstituted Complex Homo sapiens
5 CTBP2 1488
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
6 PPARG 5468
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
7 TEX11  
Two-hybrid Homo sapiens
8 HDAC9  
Reconstituted Complex Homo sapiens
9 NR3C1 2908
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 TRAF2  
Two-hybrid Homo sapiens
11 HDAC1 3065
Affinity Capture-MS Homo sapiens
12 CTBP1 1487
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
13 NR1H3  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
14 THRA  
Reconstituted Complex Homo sapiens
15 JUN 3725
Reconstituted Complex Homo sapiens
16 Ppara  
Two-hybrid Rattus norvegicus
17 RXRA  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
18 Cep70  
Two-hybrid Homo sapiens
19 PPARA  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
20 AHR 196
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 RXRB  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
22 DIPA  
Two-hybrid Homo sapiens
23 LDOC1  
Two-hybrid Homo sapiens
24 YWHAQ 10971
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Co-localization Homo sapiens
25 NR5A1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 TNFRSF14  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
27 HDAC5 10014
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
View the network image/svg+xml



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