Gene description for NRIP1
Gene name nuclear receptor interacting protein 1
Gene symbol NRIP1
Other names/aliases RIP140
Species Homo sapiens
 Database cross references - NRIP1
ExoCarta ExoCarta_8204
Vesiclepedia VP_8204
Entrez Gene 8204
HGNC 8001
MIM 602490
UniProt P48552  
 NRIP1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NRIP1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    transcription coactivator activity GO:0003713 IBA
    transcription coactivator activity GO:0003713 IDA
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 IDA
    signaling receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    nuclear receptor binding GO:0016922 IPI
    nuclear estrogen receptor binding GO:0030331 IBA
    nuclear estrogen receptor binding GO:0030331 IPI
    nuclear glucocorticoid receptor binding GO:0035259 IBA
    nuclear glucocorticoid receptor binding GO:0035259 IPI
    histone deacetylase binding GO:0042826 IEA
    nuclear retinoid X receptor binding GO:0046965 IBA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    ovarian follicle rupture GO:0001543 IEA
    circadian rhythm GO:0007623 IEP
    lipid storage GO:0019915 IEA
    circadian regulation of gene expression GO:0032922 IBA
    circadian regulation of gene expression GO:0032922 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    cellular response to estradiol stimulus GO:0071392 IBA
    cellular response to estradiol stimulus GO:0071392 IDA
Subcellular Localization
    histone deacetylase complex GO:0000118 IEA
    chromatin GO:0000785 IDA
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IEA
 Experiment description of studies that identified NRIP1 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NRIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARPC3 10094
Two-hybrid Homo sapiens
2 FHL1 2273
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 NR0B1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 ESR2  
FRET Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 RARA 5914
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 S100P 6286
Affinity Capture-MS Homo sapiens
7 TRAF2 7186
Two-hybrid Homo sapiens
8 TP53 7157
Affinity Capture-MS Homo sapiens
9 UPF1 5976
Affinity Capture-MS Homo sapiens
10 CDK8 1024
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 KIF14 9928
Affinity Capture-MS Homo sapiens
12 ESR1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Two-hybrid Homo sapiens
13 THRB 7068
Two-hybrid Homo sapiens
14 VPS33A 65082
Affinity Capture-MS Homo sapiens
15 CCDC85B  
Two-hybrid Homo sapiens
16 CTBP2 1488
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 PPARG 5468
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
18 XPO1 7514
Affinity Capture-Western Homo sapiens
19 E2F1 1869
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
20 TEX11  
Two-hybrid Homo sapiens
21 S100A2 6273
Affinity Capture-MS Homo sapiens
22 BRCA1 672
Two-hybrid Homo sapiens
23 KPNB1 3837
Affinity Capture-Western Homo sapiens
24 NR3C1 2908
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
25 HNF4A  
Two-hybrid Homo sapiens
26 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 TNNC2  
Affinity Capture-MS Homo sapiens
29 MAGEA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PRPF40A 55660
Affinity Capture-MS Homo sapiens
31 CTBP1 1487
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 CIB1 10519
Two-hybrid Homo sapiens
33 NR1H3  
Two-hybrid Homo sapiens
34 RORB  
Two-hybrid Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 BHLHB9 80823
Two-hybrid Homo sapiens
37 NR5A2  
Two-hybrid Homo sapiens
38 THRA  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
39 CWF19L2  
Affinity Capture-MS Homo sapiens
40 JUN 3725
Reconstituted Complex Homo sapiens
41 Ppara  
Two-hybrid Rattus norvegicus
42 VDR  
Two-hybrid Homo sapiens
43 TRIM37  
Proximity Label-MS Homo sapiens
44 HDAC3 8841
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
45 ESRRB  
Reconstituted Complex Homo sapiens
46 Rxrb  
Reconstituted Complex Mus musculus
47 CARM1 10498
Affinity Capture-Western Homo sapiens
48 ACTN2 88
Two-hybrid Homo sapiens
49 CEP70  
Two-hybrid Homo sapiens
50 NR3C2  
Affinity Capture-Western Homo sapiens
51 PPARA  
Reconstituted Complex Homo sapiens
52 ESRRA  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
53 RXRA 6256
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
54 AHR 196
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
55 SCGN  
Affinity Capture-MS Homo sapiens
56 CIT 11113
Affinity Capture-MS Homo sapiens
57 ESRRG  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
58 LDOC1  
Two-hybrid Homo sapiens
59 PRC1 9055
Affinity Capture-MS Homo sapiens
60 YWHAQ 10971
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Co-localization Homo sapiens
61 S100B 6285
Affinity Capture-MS Homo sapiens
62 RXRB 6257
Reconstituted Complex Homo sapiens
63 NR5A1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
64 PPARGC1A  
Affinity Capture-Western Homo sapiens
65 PRMT1 3276
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
66 NR1H2  
Two-hybrid Homo sapiens
67 HDAC5 10014
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
68 PKNOX2  
Two-hybrid Homo sapiens
69 Rara  
Reconstituted Complex Mus musculus
70 KIF20A 10112
Affinity Capture-MS Homo sapiens
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