Gene description for RARA
Gene name retinoic acid receptor, alpha
Gene symbol RARA
Other names/aliases NR1B1
RAR
Species Homo sapiens
 Database cross references - RARA
ExoCarta ExoCarta_5914
Entrez Gene 5914
HGNC 9864
MIM 180240
UniProt P10276  
 RARA identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for RARA
Molecular Function
    protein kinase B binding GO:0043422 IPI
    transcription corepressor activity GO:0003714 IDA
    mRNA 5'-UTR binding GO:0048027 IEA
    retinoic acid-responsive element binding GO:0044323 IDA
    drug binding GO:0008144 IEA
    enzyme binding GO:0019899 IPI
    phosphatidylinositol 3-kinase regulator activity GO:0035014 IEA
    receptor binding GO:0005102 IDA
    steroid hormone receptor activity GO:0003707 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    alpha-actinin binding GO:0051393 IPI
    protein domain specific binding GO:0019904 IPI
    zinc ion binding GO:0008270 IEA
    protein heterodimerization activity GO:0046982 IDA
    retinoic acid receptor activity GO:0003708 IDA
    protein kinase A binding GO:0051018 IDA
    translation repressor activity, nucleic acid binding GO:0000900 IEA
    protein binding GO:0005515 IPI
    transcription factor binding GO:0008134 IPI
    transcription coactivator activity GO:0003713 IMP
    chromatin DNA binding GO:0031490 IDA
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IEA
    retinoic acid binding GO:0001972 IDA
Biological Process
    signal transduction GO:0007165 IDA
    steroid hormone mediated signaling pathway GO:0043401 IEA
    positive regulation of binding GO:0051099 IMP
    prostate gland development GO:0030850 IEA
    response to vitamin A GO:0033189 IEA
    response to cytokine GO:0034097 IEA
    ureteric bud development GO:0001657 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    Sertoli cell fate commitment GO:0060010 IEA
    embryonic camera-type eye development GO:0031076 IEA
    positive regulation of neuron differentiation GO:0045666 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IEA
    neural tube closure GO:0001843 IEA
    face development GO:0060324 IEA
    positive regulation of cell proliferation GO:0008284 IMP
    negative regulation of tumor necrosis factor production GO:0032720 IDA
    negative regulation of cartilage development GO:0061037 IEA
    negative regulation of translational initiation GO:0045947 IEA
    female pregnancy GO:0007565 IEA
    retinoic acid receptor signaling pathway GO:0048384 IMP
    apoptotic cell clearance GO:0043277 IMP
    germ cell development GO:0007281 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    gene expression GO:0010467 TAS
    response to estradiol GO:0032355 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    negative regulation of granulocyte differentiation GO:0030853 IDA
    trachea cartilage development GO:0060534 IEA
    protein phosphorylation GO:0006468 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    spermatogenesis GO:0007283 IEA
    cellular response to retinoic acid GO:0071300 IDA
    negative regulation of interferon-gamma production GO:0032689 IDA
    positive regulation of T-helper 2 cell differentiation GO:0045630 IDA
    outflow tract septum morphogenesis GO:0003148 IEA
    cellular response to estrogen stimulus GO:0071391 IDA
    positive regulation of interleukin-4 production GO:0032753 IDA
    positive regulation of interleukin-13 production GO:0032736 IDA
    response to ethanol GO:0045471 IEA
    chondroblast differentiation GO:0060591 IEA
    regulation of myelination GO:0031641 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    positive regulation of protein kinase B signaling GO:0051897 IEA
    limb development GO:0060173 IEA
    glandular epithelial cell development GO:0002068 IEA
    positive regulation of interleukin-5 production GO:0032754 IDA
    growth plate cartilage development GO:0003417 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    multicellular organism growth GO:0035264 IEA
    positive regulation of cell cycle GO:0045787 IMP
    ventricular cardiac muscle cell differentiation GO:0055012 IEA
    response to retinoic acid GO:0032526 IMP
    regulation of synaptic plasticity GO:0048167 IEA
    liver development GO:0001889 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    regulation of phosphatidylinositol 3-kinase activity GO:0043551 IEA
Subcellular Localization
    nuclear chromatin GO:0000790 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cell surface GO:0009986 IC
    actin cytoskeleton GO:0015629 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    neuronal cell body GO:0043025 IEA
    dendrite GO:0030425 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified RARA in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RARA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK7  
Affinity Capture-MS Homo sapiens
2 NCOA6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
3 HDAC4  
Reconstituted Complex Homo sapiens
4 RARA 5914
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 Gadd45b  
Reconstituted Complex Mus musculus
6 TIF1  
Invivo Homo sapiens
7 IRX4  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
8 NR4A2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 TADA3L  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
10 GADD45G  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
11 GADD45A  
Affinity Capture-Western Homo sapiens
12 SP1  
Invivo Homo sapiens
Invitro Homo sapiens
13 GATA2  
Affinity Capture-MS Homo sapiens
14 PCAF  
Affinity Capture-Western Homo sapiens
15 ZBTB16  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 POU2F1  
Reconstituted Complex Homo sapiens
17 PNRC2  
Affinity Capture-MS Homo sapiens
18 NCOR2  
Affinity Capture-Western Homo sapiens
19 NPAS2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
20 NR0B2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
21 NR2E3  
Invitro Homo sapiens
22 SRC 6714
Reconstituted Complex Homo sapiens
23 KLF5  
Invivo Homo sapiens
Invitro Homo sapiens
24 RXRA  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
25 HDAC3 8841
Reconstituted Complex Homo sapiens
26 NCOR1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
27 BAG1 573
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
28 PML  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
29 RXRB  
Two-hybrid Homo sapiens
30 NRBF2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
31 RXRG  
Two-hybrid Homo sapiens
32 CGI-63  
Two-hybrid Homo sapiens
33 NRIP1 8204
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 COPS2 9318
Affinity Capture-MS Homo sapiens
35 NR1H3  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
36 TITF1  
Two-hybrid Homo sapiens
37 TDG 6996
Reconstituted Complex Homo sapiens
38 TRIP4  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
39 Nsd1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
40 JARID1A  
Reconstituted Complex Homo sapiens
41 PPARBP  
Invivo Homo sapiens
Invitro Homo sapiens
42 Nr0b2  
Two-hybrid Mus musculus
43 CCND3  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RARA is involved
PathwayEvidenceSource
Nuclear Receptor transcription pathway TAS Reactome
Signaling by Retinoic Acid TAS Reactome





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