Gene description for ATP8B2
Gene name ATPase, aminophospholipid transporter, class I, type 8B, member 2
Gene symbol ATP8B2
Other names/aliases ATPID
Species Homo sapiens
 Database cross references - ATP8B2
ExoCarta ExoCarta_57198
Vesiclepedia VP_57198
Entrez Gene 57198
HGNC 13534
MIM 605867
UniProt P98198  
 ATP8B2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ATP8B2
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    phosphatidylcholine floppase activity GO:0090554 IEA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 IBA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 TAS
    phosphatidylcholine flippase activity GO:0140345 IDA
Biological Process
    Golgi organization GO:0007030 IBA
    monoatomic ion transmembrane transport GO:0034220 TAS
    phospholipid translocation GO:0045332 IBA
Subcellular Localization
    nucleolus GO:0005730 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    phospholipid-translocating ATPase complex GO:1990531 IPI
 Experiment description of studies that identified ATP8B2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ATP8B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 NKAIN1  
Affinity Capture-MS Homo sapiens
3 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KCNMB3  
Affinity Capture-MS Homo sapiens
5 RARA 5914
Affinity Capture-MS Homo sapiens
6 RAMP2  
Affinity Capture-MS Homo sapiens
7 SLC6A15 55117
Affinity Capture-MS Homo sapiens
8 CXCR4 7852
Affinity Capture-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 ATP8B4  
Affinity Capture-MS Homo sapiens
11 RXFP1  
Affinity Capture-MS Homo sapiens
12 CD3D 915
Affinity Capture-MS Homo sapiens
13 SLC39A4 55630
Affinity Capture-MS Homo sapiens
14 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HCST  
Affinity Capture-MS Homo sapiens
16 LPAR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
20 CRELD1 78987
Affinity Capture-MS Homo sapiens
21 CX3CL1  
Affinity Capture-MS Homo sapiens
22 B4GAT1 11041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MAMDC4  
Affinity Capture-MS Homo sapiens
24 KLRD1  
Affinity Capture-MS Homo sapiens
25 ARF6 382
Proximity Label-MS Homo sapiens
26 CLEC4C  
Affinity Capture-MS Homo sapiens
27 FAXC  
Affinity Capture-MS Homo sapiens
28 TMEM169  
Affinity Capture-MS Homo sapiens
29 TIGIT  
Affinity Capture-MS Homo sapiens
30 KITLG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SLC22A2 6582
Affinity Capture-MS Homo sapiens
32 TMED2 10959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CHCHD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CCR6  
Affinity Capture-MS Homo sapiens
36 SLC22A4 6583
Affinity Capture-MS Homo sapiens
37 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 DGCR2 9993
Affinity Capture-MS Homo sapiens
39 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATP8B2 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Transport of small molecules TAS Reactome





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