Gene description for ARID1A
Gene name AT rich interactive domain 1A (SWI-like)
Gene symbol ARID1A
Other names/aliases B120
BAF250
BAF250a
BM029
C1orf4
ELD
MRD14
OSA1
P270
SMARCF1
hELD
hOSA1
Species Homo sapiens
 Database cross references - ARID1A
ExoCarta ExoCarta_8289
Entrez Gene 8289
HGNC 11110
MIM 603024
UniProt O14497  
 ARID1A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
Prostate cancer cells 25844599    
 Gene ontology annotations for ARID1A
Molecular Function
    DNA binding GO:0003677 NAS
    ligand-dependent nuclear receptor binding GO:0016922 IPI
    transcription coactivator activity GO:0003713 NAS
    nucleosome binding GO:0031491 IEA
    protein binding GO:0005515 IPI
Biological Process
    androgen receptor signaling pathway GO:0030521 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    optic cup formation involved in camera-type eye development GO:0003408 IEA
    nucleosome mobilization GO:0042766 TAS
    ATP-dependent chromatin remodeling GO:0043044 IEA
    intracellular estrogen receptor signaling pathway GO:0030520 IDA
    cardiac chamber development GO:0003205 IEA
    chromatin remodeling GO:0006338 IDA
    glucocorticoid receptor signaling pathway GO:0042921 IDA
    nucleosome disassembly GO:0006337 IDA
    placenta blood vessel development GO:0060674 IEA
    transcription, DNA-templated GO:0006351 IEA
    chromatin-mediated maintenance of transcription GO:0048096 TAS
    neural tube closure GO:0001843 IEA
    forebrain development GO:0030900 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 NAS
    toxin transport GO:1901998 IEA
Subcellular Localization
    SWI/SNF complex GO:0016514 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nBAF complex GO:0071565 ISS
    npBAF complex GO:0071564 ISS
    nuclear chromatin GO:0000790 IDA
 Experiment description of studies that identified ARID1A in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
5
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for ARID1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Reconstituted Complex Homo sapiens
2 SMARCA2  
Co-purification Homo sapiens
3 SMARCA4 6597
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
4 KLF1  
Reconstituted Complex Homo sapiens
5 SMARCB1 6598
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
6 ING1  
Affinity Capture-MS Homo sapiens
7 BAZ1B 9031
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ARID1A is involved
PathwayEvidenceSource
RMTs methylate histone arginines TAS Reactome





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