Gene description for MBD2
Gene name methyl-CpG binding domain protein 2
Gene symbol MBD2
Other names/aliases DMTase
NY-CO-41
Species Homo sapiens
 Database cross references - MBD2
ExoCarta ExoCarta_8932
Vesiclepedia VP_8932
Entrez Gene 8932
HGNC 6917
MIM 603547
UniProt Q9UBB5  
 MBD2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MBD2
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IDA
    protein domain specific binding GO:0019904 IPI
    methyl-CpG binding GO:0008327 NAS
    nucleosomal DNA binding GO:0031492 IDA
    mRNA binding GO:0003729 IEA
    C2H2 zinc finger domain binding GO:0070742 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    satellite DNA binding GO:0003696 TAS
    siRNA binding GO:0035197 IEA
Biological Process
    ATP-dependent chromatin remodeling GO:0043044 IDA
    maternal behavior GO:0042711 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    cellular protein complex assembly GO:0043623 IEA
    regulation of cell proliferation GO:0042127 IEA
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    regulation of gene expression, epigenetic GO:0040029 TAS
    transcription, DNA-templated GO:0006351 IEA
    negative regulation of transcription, DNA-templated GO:0045892 NAS
    gene expression GO:0010467 TAS
    positive regulation of Wnt signaling pathway GO:0030177 IEA
Subcellular Localization
    histone deacetylase complex GO:0000118 IEA
    heterochromatin GO:0000792 IEA
    cytoplasm GO:0005737 IEA
    nuclear chromatin GO:0000790 IDA
    protein complex GO:0043234 IDA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 NAS
 Experiment description of studies that identified MBD2 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MBD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATAD2A 54815
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 XAB1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 DHX9 1660
Two-hybrid Homo sapiens
4 GATAD2B 57459
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 MBD3 53615
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
6 HDAC2 3066
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 MIZF  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 MTA2 9219
Reconstituted Complex Homo sapiens
9 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 SIN3A  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
11 CHD3 1107
Reconstituted Complex Homo sapiens
12 MBD3L1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 RBBP4 5928
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MBD2 is involved
PathwayEvidenceSource
NoRC negatively regulates rRNA expression IEA Reactome
RNA Polymerase I Promoter Opening TAS Reactome





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