Gene description for RFX3
Gene name regulatory factor X, 3 (influences HLA class II expression)
Gene symbol RFX3
Other names/aliases -
Species Homo sapiens
 Database cross references - RFX3
ExoCarta ExoCarta_5991
Entrez Gene 5991
HGNC 9984
MIM 601337
UniProt P48380  
 RFX3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RFX3
Molecular Function
    sequence-specific DNA binding transcription factor activity GO:0003700 NAS
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity GO:0003705 TAS
    DNA binding GO:0003677 TAS
    protein binding GO:0005515 IPI
    transcription regulatory region DNA binding GO:0044212 IDA
Biological Process
    cell maturation GO:0048469 ISS
    cilium-dependent cell motility GO:0060285 ISS
    regulation of transcription from RNA polymerase II promoter GO:0006357 IBA
    regulation of transcription, DNA-templated GO:0006355 ISS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    positive regulation of type B pancreatic cell development GO:2000078 ISS
    cilium assembly GO:0042384 ISS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    epithelial cilium movement involved in determination of left/right asymmetry GO:0060287 ISS
    endocrine pancreas development GO:0031018 ISS
    regulation of insulin secretion GO:0050796 ISS
    type B pancreatic cell maturation GO:0072560 IEA
Subcellular Localization
    nuclear chromatin GO:0000790 IDA
    transcription factor complex GO:0005667 IC
    nucleus GO:0005634 IC
 Experiment description of studies that identified RFX3 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RFX3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RFXDC1  
Two-hybrid Homo sapiens
2 TRAF2  
Two-hybrid Homo sapiens
3 RFX4  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 RFX3 5991
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 RFX1  
Invitro Homo sapiens
6 RFX2  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RFX3 is involved
No pathways found





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