Gene description for INPP5K
Gene name inositol polyphosphate-5-phosphatase K
Gene symbol INPP5K
Other names/aliases PPS
SKIP
Species Homo sapiens
 Database cross references - INPP5K
ExoCarta ExoCarta_51763
Vesiclepedia VP_51763
Entrez Gene 51763
HGNC 33882
MIM 607875
UniProt Q9BT40  
 INPP5K identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for INPP5K
Molecular Function
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IEA
    lipid phosphatase activity GO:0042577 NAS
    phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity GO:0034485 IDA
    protein binding GO:0005515 IPI
    inositol bisphosphate phosphatase activity GO:0016312 IDA
    phosphatidylinositol trisphosphate phosphatase activity GO:0034594 IDA
    vasopressin receptor activity GO:0005000 ISS
    inositol-polyphosphate 5-phosphatase activity GO:0004445 IEA
    inositol trisphosphate phosphatase activity GO:0046030 IDA
    phosphatidylinositol phosphate 5-phosphatase activity GO:0034595 IMP
    inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity GO:0052659 IEA
Biological Process
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    cellular response to tumor necrosis factor GO:0071356 IDA
    negative regulation of calcium ion transport GO:0051926 IDA
    negative regulation of glycogen (starch) synthase activity GO:2000466 ISS
    negative regulation of glucose transport GO:0010829 IDA
    negative regulation of protein kinase activity GO:0006469 IDA
    actin cytoskeleton organization GO:0030036 NAS
    negative regulation of MAP kinase activity GO:0043407 IDA
    positive regulation of renal water transport GO:2001153 ISS
    negative regulation of glycogen biosynthetic process GO:0045719 IDA
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IDA
    cellular response to epidermal growth factor stimulus GO:0071364 IDA
    G-protein coupled receptor signaling pathway GO:0007186 ISS
    negative regulation of protein targeting to membrane GO:0090315 IDA
    in utero embryonic development GO:0001701 IEA
    positive regulation of urine volume GO:0035810 ISS
    negative regulation of protein kinase B signaling GO:0051898 IDA
    negative regulation of insulin receptor signaling pathway GO:0046627 IDA
    negative regulation of stress fiber assembly GO:0051497 IDA
    negative regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045869 IDA
    regulation of glycogen biosynthetic process GO:0005979 ISS
    glucose homeostasis GO:0042593 ISS
    small molecule metabolic process GO:0044281 TAS
    negative regulation of dephosphorylation GO:0035305 ISS
    dephosphorylation GO:0016311 IMP
    cellular response to hormone stimulus GO:0032870 ISS
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    cellular response to insulin stimulus GO:0032869 IDA
    protein targeting to plasma membrane GO:0072661 ISS
    cellular response to cAMP GO:0071320 ISS
    negative regulation of protein phosphorylation GO:0001933 ISS
    ruffle assembly GO:0097178 IDA
    inositol phosphate dephosphorylation GO:0046855 IDA
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IDA
    phospholipid metabolic process GO:0006644 TAS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    negative regulation by host of viral transcription GO:0043922 IDA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    trans-Golgi network GO:0005802 IDA
    ruffle membrane GO:0032587 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    ruffle GO:0001726 IDA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 IDA
    neuron projection GO:0043005 IDA
 Experiment description of studies that identified INPP5K in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for INPP5K
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARL6IP1 23204
Two-hybrid Homo sapiens
2 NCOR2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which INPP5K is involved
PathwayEvidenceSource
Synthesis of PIPs at the plasma membrane TAS Reactome





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