Gene description for DOCK1
Gene name dedicator of cytokinesis 1
Gene symbol DOCK1
Other names/aliases DOCK180
ced5
Species Homo sapiens
 Database cross references - DOCK1
ExoCarta ExoCarta_1793
Entrez Gene 1793
HGNC 2987
MIM 601403
UniProt Q14185  
 DOCK1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for DOCK1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 IEA
    SH3 domain binding GO:0017124 IEA
Biological Process
    cell migration GO:0016477 IEA
    blood coagulation GO:0007596 TAS
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    small GTPase mediated signal transduction GO:0007264 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    cytoskeleton organization GO:0007010 IEA
    phagocytosis, engulfment GO:0006911 TAS
    positive regulation of GTPase activity GO:0043547 TAS
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    integrin-mediated signaling pathway GO:0007229 TAS
    innate immune response GO:0045087 TAS
    axon guidance GO:0007411 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    membrane GO:0016020 IEA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DOCK1 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOCK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ELMO2 63916
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 NCK2 8440
Reconstituted Complex Homo sapiens
3 CRK 1398
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 SRC 6714
Affinity Capture-Western Homo sapiens
5 BCAR1 9564
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 RAC1 5879
Reconstituted Complex Homo sapiens
7 ELMO1 9844
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 RHOG 391
Affinity Capture-Western Homo sapiens
9 GRB2 2885
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
10 PTK2 5747
Affinity Capture-Western Homo sapiens
11 WASF2 10163
Invivo Homo sapiens
Invitro Homo sapiens
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