Gene description for ACTL6A
Gene name actin-like 6A
Gene symbol ACTL6A
Other names/aliases ACTL6
ARPN-BETA
Arp4
BAF53A
INO80K
Species Homo sapiens
 Database cross references - ACTL6A
ExoCarta ExoCarta_86
Entrez Gene 86
HGNC 24124
MIM 604958
UniProt O96019  
 ACTL6A identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for ACTL6A
Molecular Function
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 TAS
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IDA
    nucleosomal DNA binding GO:0031492 IDA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    transcription coactivator activity GO:0003713 NAS
Biological Process
    DNA recombination GO:0006310 IEA
    chromatin organization GO:0006325 TAS
    regulation of mitochondrion degradation GO:1903146 IMP
    signal transduction GO:0007165 TAS
    ATP-dependent chromatin remodeling GO:0043044 IDA
    histone H4 acetylation GO:0043967 IDA
    transcription, DNA-templated GO:0006351 IEA
    histone H2A acetylation GO:0043968 IDA
    nervous system development GO:0007399 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 NAS
    DNA repair GO:0006281 IEA
    neural retina development GO:0003407 IEP
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    regulation of growth GO:0040008 IEA
    chromatin remodeling GO:0006338 IDA
Subcellular Localization
    npBAF complex GO:0071564 ISS
    nucleoplasm GO:0005654 TAS
    plasma membrane GO:0005886 IDA
    nucleus GO:0005634 IDA
    nuclear chromatin GO:0000790 IDA
    SWI/SNF complex GO:0016514 IDA
    NuA4 histone acetyltransferase complex GO:0035267 IDA
    protein complex GO:0043234 IDA
    Ino80 complex GO:0031011 IDA
 Experiment description of studies that identified ACTL6A in exosomes
1
Experiment ID 76
ISEV standards
EM
EV Biophysical techniques
TSG101|GAPDH|HSP90
EV Cytosolic markers
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACTL6A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGDIA 396
Two-hybrid Homo sapiens
2 GCN5L2  
Co-fractionation Homo sapiens
3 SMARCA2  
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
4 SMARCA4 6597
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
5 SMARCE1 6605
Affinity Capture-Western Homo sapiens
6 RUVBL1 8607
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
7 SMARCB1 6598
Co-purification Homo sapiens
8 HTATIP  
Co-fractionation Homo sapiens
9 MYC  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
10 ING1  
Affinity Capture-MS Homo sapiens
11 EP400  
Affinity Capture-MS Homo sapiens
12 BAZ1B 9031
Affinity Capture-MS Homo sapiens
13 EWSR1 2130
Two-hybrid Homo sapiens
14 RELB  
Affinity Capture-MS Homo sapiens
15 TRRAP 8295
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ACTL6A is involved
PathwayEvidenceSource
HATs acetylate histones TAS Reactome
RMTs methylate histone arginines TAS Reactome





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