Gene description for RAP1BL
Gene name RAP1B, member of RAS oncogene family pseudogene
Gene symbol RAP1BL
Other names/aliases -
Species Homo sapiens
 Database cross references - RAP1BL
ExoCarta ExoCarta_643752
Vesiclepedia VP_643752
Entrez Gene 643752
 RAP1BL identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Urine 19056867    
 Gene ontology annotations for RAP1BL
Molecular Function
    GTPase activity GO:0003924 IBA
    GTP binding GO:0005525 IBA
    GDP binding GO:0019003 IBA
Biological Process
    Rap protein signal transduction GO:0032486 IBA
    cellular response to cAMP GO:0071320 IBA
    negative regulation of synaptic vesicle exocytosis GO:2000301 IBA
Subcellular Localization
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified RAP1BL in exosomes
1
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 21
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101|HSP70|CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RAP1BL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WIF1 11197
Affinity Capture-MS Homo sapiens
2 CD53 963
Affinity Capture-MS Homo sapiens
3 SERBP1 26135
Affinity Capture-MS Homo sapiens
4 TMEM257  
Affinity Capture-MS Homo sapiens
5 RPS27 6232
Affinity Capture-MS Homo sapiens
6 CPA5  
Affinity Capture-MS Homo sapiens
7 KLRC4  
Affinity Capture-MS Homo sapiens
8 PER2  
Proximity Label-MS Homo sapiens
9 CCR1  
Affinity Capture-MS Homo sapiens
10 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
11 CERS2 29956
Affinity Capture-MS Homo sapiens
12 MRPL42  
Affinity Capture-MS Homo sapiens
13 HBG2 3048
Affinity Capture-MS Homo sapiens
14 KIF23 9493
Affinity Capture-MS Homo sapiens
15 ACTL6A 86
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DDX55  
Affinity Capture-MS Homo sapiens
17 DUXAP10  
Affinity Capture-MS Homo sapiens
18 DYX1C1  
Affinity Capture-MS Homo sapiens
19 SERPINB5 5268
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RAP1BL is involved
No pathways found





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