Gene description for HECTD1
Gene name HECT domain containing E3 ubiquitin protein ligase 1
Gene symbol HECTD1
Other names/aliases EULIR
Species Homo sapiens
 Database cross references - HECTD1
ExoCarta ExoCarta_25831
Entrez Gene 25831
HGNC 20157
UniProt Q9ULT8  
 HECTD1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for HECTD1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IBA
    ligase activity GO:0016874 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    neural tube closure GO:0001843 IEA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified HECTD1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for HECTD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which HECTD1 is involved
No pathways found





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