Gene description for HECTD1
Gene name HECT domain containing E3 ubiquitin protein ligase 1
Gene symbol HECTD1
Other names/aliases EULIR
Species Homo sapiens
 Database cross references - HECTD1
ExoCarta ExoCarta_25831
Vesiclepedia VP_25831
Entrez Gene 25831
HGNC 20157
UniProt Q9ULT8  
 HECTD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HECTD1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 ISS
Biological Process
    heart valve development GO:0003170 ISS
    ventricular septum development GO:0003281 ISS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    aorta development GO:0035904 ISS
 Experiment description of studies that identified HECTD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HECTD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPATS2  
Affinity Capture-MS Homo sapiens
2 SLC25A13 10165
Affinity Capture-MS Homo sapiens
3 GAR1 54433
Affinity Capture-MS Homo sapiens
4 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ZNF771  
Affinity Capture-MS Homo sapiens
6 ZBTB11  
Affinity Capture-MS Homo sapiens
7 AATF  
Affinity Capture-MS Homo sapiens
8 SMC5 23137
Affinity Capture-MS Homo sapiens
9 ZNF346  
Affinity Capture-MS Homo sapiens
10 EXOSC3 51010
Affinity Capture-MS Homo sapiens
11 RIN3  
Affinity Capture-MS Homo sapiens
12 SRPK2 6733
Affinity Capture-MS Homo sapiens
13 NOP14 8602
Affinity Capture-MS Homo sapiens
14 USP10 9100
Affinity Capture-MS Homo sapiens
15 TPM2 7169
Co-fractionation Homo sapiens
16 CDCA2 157313
Affinity Capture-MS Homo sapiens
17 DDX17 10521
Affinity Capture-MS Homo sapiens
18 NMNAT1  
Affinity Capture-MS Homo sapiens
19 MAP4 4134
Affinity Capture-MS Homo sapiens
20 UBC 7316
Affinity Capture-MS Homo sapiens
21 GTPBP1 9567
Affinity Capture-MS Homo sapiens
22 NHP2L1 4809
Affinity Capture-MS Homo sapiens
23 KPNA2 3838
Affinity Capture-MS Homo sapiens
24 MRPS27 23107
Affinity Capture-MS Homo sapiens
25 GCN1L1 10985
Affinity Capture-MS Homo sapiens
26 PRKDC 5591
Affinity Capture-MS Homo sapiens
27 AKAP8L  
Affinity Capture-MS Homo sapiens
28 TACC2 10579
Affinity Capture-MS Homo sapiens
29 CAPN6  
Affinity Capture-MS Homo sapiens
30 TCEB3 6924
Affinity Capture-MS Homo sapiens
31 CUL7 9820
Affinity Capture-MS Homo sapiens
32 CENPU  
Affinity Capture-MS Homo sapiens
33 GPATCH4 54865
Affinity Capture-MS Homo sapiens
34 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
35 SNRNP70 6625
Affinity Capture-MS Homo sapiens
36 TRMT1L 81627
Affinity Capture-MS Homo sapiens
37 SAMHD1 25939
Affinity Capture-MS Homo sapiens
38 RBM28 55131
Affinity Capture-MS Homo sapiens
39 DPM1 8813
Affinity Capture-MS Homo sapiens
40 DARS 1615
Affinity Capture-MS Homo sapiens
41 KIF23 9493
Affinity Capture-MS Homo sapiens
42 PPP2R5D 5528
Co-fractionation Homo sapiens
43 AP2A1 160
Affinity Capture-MS Homo sapiens
44 AGGF1  
Affinity Capture-MS Homo sapiens
45 CDH2 1000
Affinity Capture-MS Homo sapiens
46 MTCH1 23787
Affinity Capture-MS Homo sapiens
47 RASSF6  
Affinity Capture-MS Homo sapiens
48 FLII 2314
Affinity Capture-MS Homo sapiens
49 FBL 2091
Affinity Capture-MS Homo sapiens
50 ATAD5  
Affinity Capture-MS Homo sapiens
51 SMC2 10592
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
52 CHMP2B 25978
Affinity Capture-MS Homo sapiens
53 RPS9 6203
Affinity Capture-MS Homo sapiens
54 MAGED2 10916
Affinity Capture-MS Homo sapiens
55 SPTY2D1  
Affinity Capture-MS Homo sapiens
56 POLRMT 5442
Affinity Capture-MS Homo sapiens
57 EIF3E 3646
Affinity Capture-MS Homo sapiens
58 FAM207A  
Affinity Capture-MS Homo sapiens
59 HERC2 8924
Affinity Capture-MS Homo sapiens
60 CENPQ  
Affinity Capture-MS Homo sapiens
61 PNMAL1  
Affinity Capture-MS Homo sapiens
62 WDR5 11091
Affinity Capture-MS Homo sapiens
63 C12orf43  
Affinity Capture-MS Homo sapiens
64 SLU7  
Affinity Capture-MS Homo sapiens
65 RPL19 6143
Affinity Capture-MS Homo sapiens
66 RBM14 10432
Affinity Capture-MS Homo sapiens
67 ACIN1 22985
Affinity Capture-MS Homo sapiens
68 EXOSC9 5393
Affinity Capture-MS Homo sapiens
69 RBMX 27316
Affinity Capture-MS Homo sapiens
70 XRCC4  
Affinity Capture-MS Homo sapiens
71 AURKA 6790
Affinity Capture-MS Homo sapiens
72 TXLNG  
Affinity Capture-MS Homo sapiens
73 TOLLIP 54472
Proximity Label-MS Homo sapiens
74 KIFC1 3833
Affinity Capture-MS Homo sapiens
75 DLAT 1737
Affinity Capture-MS Homo sapiens
76 UTP14A 10813
Affinity Capture-MS Homo sapiens
77 RBM12B  
Affinity Capture-MS Homo sapiens
78 RFC5 5985
Affinity Capture-MS Homo sapiens
79 TOP2B 7155
Affinity Capture-MS Homo sapiens
80 UPF2 26019
Affinity Capture-MS Homo sapiens
81 TARBP2  
Affinity Capture-MS Homo sapiens
82 MCM9 254394
Affinity Capture-MS Homo sapiens
83 RRP9 9136
Affinity Capture-MS Homo sapiens
84 C17orf85  
Affinity Capture-MS Homo sapiens
85 C19orf47  
Affinity Capture-MS Homo sapiens
86 RCL1 10171
Affinity Capture-MS Homo sapiens
87 RPL18A 6142
Affinity Capture-MS Homo sapiens
88 LRRFIP1 9208
Affinity Capture-MS Homo sapiens
89 SRBD1  
Affinity Capture-MS Homo sapiens
90 EXOSC4 54512
Affinity Capture-MS Homo sapiens
91 CKAP5 9793
Affinity Capture-MS Homo sapiens
92 RPL31 6160
Affinity Capture-MS Homo sapiens
93 SPRYD3 84926
Affinity Capture-MS Homo sapiens
94 RBM17 84991
Affinity Capture-MS Homo sapiens
95 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
96 CXorf57  
Affinity Capture-MS Homo sapiens
97 YWHAQ 10971
Affinity Capture-MS Homo sapiens
98 RFC4 5984
Affinity Capture-MS Homo sapiens
99 HECTD1 25831
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
100 RRP1B 23076
Affinity Capture-MS Homo sapiens
101 SON 6651
Affinity Capture-MS Homo sapiens
102 TFAM 7019
Affinity Capture-MS Homo sapiens
103 PRPF31 26121
Affinity Capture-MS Homo sapiens
104 SND1 27044
Affinity Capture-MS Homo sapiens
105 GTPBP4 23560
Affinity Capture-MS Homo sapiens
106 IQGAP2 10788
Affinity Capture-MS Homo sapiens
107 SRPK1 6732
Affinity Capture-MS Homo sapiens
108 CD3EAP  
Affinity Capture-MS Homo sapiens
109 ZNF326 284695
Affinity Capture-MS Homo sapiens
110 TMPO 7112
Affinity Capture-MS Homo sapiens
111 GTF3C3 9330
Affinity Capture-MS Homo sapiens
112 CBX8 57332
Affinity Capture-MS Homo sapiens
113 MED17  
Affinity Capture-MS Homo sapiens
114 DNAJC21  
Affinity Capture-MS Homo sapiens
115 NOC3L 64318
Affinity Capture-MS Homo sapiens
116 DCAF7 10238
Affinity Capture-MS Homo sapiens
117 MRTO4 51154
Affinity Capture-MS Homo sapiens
118 AHCYL1 10768
Co-fractionation Homo sapiens
119 MARK3 4140
Affinity Capture-MS Homo sapiens
120 CCDC101  
Affinity Capture-MS Homo sapiens
121 LXN 56925
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
122 SIRT7  
Affinity Capture-MS Homo sapiens
123 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
124 CD2BP2 10421
Affinity Capture-MS Homo sapiens
125 ZNF622 90441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 IQCB1  
Affinity Capture-MS Homo sapiens
127 ANXA1 301
Affinity Capture-MS Homo sapiens
128 ACAD11 84129
Affinity Capture-MS Homo sapiens
129 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 YWHAZ 7534
Affinity Capture-MS Homo sapiens
131 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
132 PAPD5 64282
Affinity Capture-MS Homo sapiens
133 NOM1  
Affinity Capture-MS Homo sapiens
134 SAMD1  
Affinity Capture-MS Homo sapiens
135 RPL7L1 285855
Affinity Capture-MS Homo sapiens
136 DHX37  
Affinity Capture-MS Homo sapiens
137 ZNF223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 RBBP4 5928
Affinity Capture-MS Homo sapiens
139 GTF3C4 9329
Affinity Capture-MS Homo sapiens
140 STRAP 11171
Affinity Capture-MS Homo sapiens
141 RBM6 10180
Affinity Capture-MS Homo sapiens
142 ZNF512  
Affinity Capture-MS Homo sapiens
143 FARSB 10056
Affinity Capture-MS Homo sapiens
144 RPA3 6119
Proximity Label-MS Homo sapiens
145 LTV1  
Affinity Capture-MS Homo sapiens
146 DLST 1743
Affinity Capture-MS Homo sapiens
147 MYO9B 4650
Affinity Capture-MS Homo sapiens
148 RPL27A 6157
Affinity Capture-MS Homo sapiens
149 AP1M2 10053
Affinity Capture-MS Homo sapiens
150 MED23 9439
Affinity Capture-MS Homo sapiens
151 RPF1  
Affinity Capture-MS Homo sapiens
152 SPATA5 166378
Affinity Capture-MS Homo sapiens
153 EXOSC2 23404
Affinity Capture-MS Homo sapiens
154 EIF2B1 1967
Affinity Capture-MS Homo sapiens
155 CSDE1 7812
Affinity Capture-MS Homo sapiens
156 SCAPER  
Affinity Capture-MS Homo sapiens
157 HEATR1 55127
Affinity Capture-MS Homo sapiens
158 MYH10 4628
Co-fractionation Homo sapiens
159 RPS15A 6210
Affinity Capture-MS Homo sapiens
160 AKAP8  
Affinity Capture-MS Homo sapiens
161 UTP3 57050
Affinity Capture-MS Homo sapiens
162 SUPT16H 11198
Affinity Capture-MS Homo sapiens
163 MED14  
Affinity Capture-MS Homo sapiens
164 GNL3 26354
Affinity Capture-MS Homo sapiens
165 DDX55  
Affinity Capture-MS Homo sapiens
166 FAM133A 286499
Affinity Capture-MS Homo sapiens
167 NGDN  
Affinity Capture-MS Homo sapiens
168 RPS16 6217
Affinity Capture-MS Homo sapiens
169 DDX46 9879
Affinity Capture-MS Homo sapiens
170 YBX1 4904
Affinity Capture-MS Homo sapiens
171 DECR1 1666
Affinity Capture-MS Homo sapiens
172 C8orf33 65265
Affinity Capture-MS Homo sapiens
173 PDK3 5165
Affinity Capture-MS Homo sapiens
174 NUP205 23165
Affinity Capture-MS Homo sapiens
175 BCAS2 10286
Affinity Capture-MS Homo sapiens
176 ARID2  
Affinity Capture-MS Homo sapiens
177 POLE  
Affinity Capture-MS Homo sapiens
178 CHTOP  
Affinity Capture-MS Homo sapiens
179 RRS1 23212
Affinity Capture-MS Homo sapiens
180 URGCP 55665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
181 MACF1 23499
Affinity Capture-MS Homo sapiens
182 RBM25 58517
Co-fractionation Homo sapiens
183 FBXO10  
Affinity Capture-MS Homo sapiens
184 RUVBL2 10856
Affinity Capture-MS Homo sapiens
185 PCMT1 5110
Affinity Capture-MS Homo sapiens
186 POLR1B 84172
Affinity Capture-MS Homo sapiens
187 ARCN1 372
Affinity Capture-MS Homo sapiens
188 SRSF9 8683
Affinity Capture-MS Homo sapiens
189 CDK1 983
Affinity Capture-MS Homo sapiens
190 COPE 11316
Affinity Capture-MS Homo sapiens
191 LSM14A 26065
Affinity Capture-MS Homo sapiens
192 CKAP2L  
Affinity Capture-MS Homo sapiens
193 RRP1 8568
Affinity Capture-MS Homo sapiens
194 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
195 SKP1 6500
Affinity Capture-MS Homo sapiens
196 EIF3I 8668
Affinity Capture-MS Homo sapiens
197 MORF4L2  
Affinity Capture-MS Homo sapiens
198 DCTN2 10540
Proximity Label-MS Homo sapiens
199 PIK3R4 30849
Affinity Capture-MS Homo sapiens
200 CDC40  
Affinity Capture-MS Homo sapiens
201 TBL3 10607
Affinity Capture-MS Homo sapiens
202 G3BP2 9908
Affinity Capture-MS Homo sapiens
203 TCERG1 10915
Affinity Capture-MS Homo sapiens
204 H2AFY 9555
Affinity Capture-MS Homo sapiens
205 SUGP2  
Affinity Capture-MS Homo sapiens
206 FAR1 84188
Affinity Capture-MS Homo sapiens
207 PWP1 11137
Affinity Capture-MS Homo sapiens
208 MED16 10025
Affinity Capture-MS Homo sapiens
209 ERCC3  
Affinity Capture-MS Homo sapiens
210 KRR1 11103
Affinity Capture-MS Homo sapiens
211 LARP7 51574
Affinity Capture-MS Homo sapiens
212 CLASP1 23332
Affinity Capture-MS Homo sapiens
213 IK 3550
Affinity Capture-MS Homo sapiens
214 RUVBL1 8607
Affinity Capture-MS Homo sapiens
215 NCL 4691
Affinity Capture-MS Homo sapiens
216 ALYREF 10189
Affinity Capture-MS Homo sapiens
217 RAD21 5885
Affinity Capture-MS Homo sapiens
218 ZNF768 79724
Affinity Capture-MS Homo sapiens
219 DHX33  
Affinity Capture-MS Homo sapiens
220 POLR3B 55703
Affinity Capture-MS Homo sapiens
221 CEP78 84131
Affinity Capture-MS Homo sapiens
222 PRPF4 9128
Affinity Capture-MS Homo sapiens
223 PPAN 56342
Affinity Capture-MS Homo sapiens
224 HMMR  
Affinity Capture-MS Homo sapiens
225 ANXA2 302
Affinity Capture-MS Homo sapiens
226 UTP6  
Affinity Capture-MS Homo sapiens
227 DNMT1 1786
Affinity Capture-MS Homo sapiens
228 PHF8  
Affinity Capture-MS Homo sapiens
229 NVL  
Affinity Capture-MS Homo sapiens
230 EXOSC8  
Affinity Capture-MS Homo sapiens
231 HERC5 51191
Affinity Capture-MS Homo sapiens
232 PSMD3 5709