Gene description for PURA
Gene name purine-rich element binding protein A
Gene symbol PURA
Other names/aliases MRD31
PUR-ALPHA
PUR1
PURALPHA
Species Homo sapiens
 Database cross references - PURA
ExoCarta ExoCarta_5813
Vesiclepedia VP_5813
Entrez Gene 5813
HGNC 9701
MIM 600473
UniProt Q00577  
 PURA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PURA
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 ISS
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 ISS
    double-stranded telomeric DNA binding GO:0003691 IDA
    single-stranded DNA binding GO:0003697 ISS
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    purine-rich negative regulatory element binding GO:0032422 IBA
    purine-rich negative regulatory element binding GO:0032422 ISS
    SMAD binding GO:0046332 IEA
    DNA-binding transcription factor binding GO:0140297 ISS
    transcription regulator inhibitor activity GO:0140416 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    DNA unwinding involved in DNA replication GO:0006268 IDA
    DNA replication initiation GO:0006270 TAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    nervous system development GO:0007399 IEA
    positive regulation of cell population proliferation GO:0008284 IEA
    negative regulation of translation GO:0017148 IEA
    lymphocyte proliferation GO:0046651 IEA
    epithelial cell proliferation GO:0050673 IEA
    dendritic transport of messenger ribonucleoprotein complex GO:0098963 IEA
Subcellular Localization
    chromosome, telomeric region GO:0000781 IC
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IEA
    dendrite GO:0030425 IEA
    neuronal cell body GO:0043025 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified PURA in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PURA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Affinity Capture-MS Homo sapiens
2 ZNF668  
Affinity Capture-MS Homo sapiens
3 RPSAP58 388524
Affinity Capture-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 RSL1D1 26156
Affinity Capture-MS Homo sapiens
6 ZNF346  
Affinity Capture-MS Homo sapiens
7 PSMD11 5717
Co-fractionation Homo sapiens
8 TARDBP 23435
Affinity Capture-MS Homo sapiens
9 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
10 SP1  
Affinity Capture-Western Homo sapiens
11 RPL13A 23521
Affinity Capture-MS Homo sapiens
12 E2F1 1869
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 RPS19 6223
Affinity Capture-MS Homo sapiens
14 RPL15 6138
Affinity Capture-MS Homo sapiens
15 UBC 7316
Affinity Capture-MS Homo sapiens
16 RPL14 9045
Affinity Capture-MS Homo sapiens
17 CAND1 55832
Affinity Capture-MS Homo sapiens
18 MCM3 4172
Co-fractionation Homo sapiens
19 RPS3 6188
Affinity Capture-MS Homo sapiens
20 USP36  
Affinity Capture-MS Homo sapiens
21 SURF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DDX23 9416
Co-fractionation Homo sapiens
23 RPL4 6124
Affinity Capture-MS Homo sapiens
24 WDR76  
Affinity Capture-MS Homo sapiens
25 PRR11  
Affinity Capture-MS Homo sapiens
26 B3GNT2 10678
Affinity Capture-MS Homo sapiens
27 LLPH  
Affinity Capture-MS Homo sapiens
28 KIF23 9493
Affinity Capture-MS Homo sapiens
29 ZNF638 27332
Co-fractionation Homo sapiens
30 FBXW7  
Affinity Capture-MS Homo sapiens
31 RFC3 5983
Co-fractionation Homo sapiens
32 POU5F1  
Affinity Capture-RNA Homo sapiens
33 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CUL4A 8451
Affinity Capture-MS Homo sapiens
35 ADARB1 104
Affinity Capture-MS Homo sapiens
36 PRKRA 8575
Affinity Capture-MS Homo sapiens
37 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
38 ATG16L1 55054
Affinity Capture-MS Homo sapiens
39 NHP2 55651
Affinity Capture-MS Homo sapiens
40 CUL1 8454
Affinity Capture-MS Homo sapiens
41 ARIH2 10425
Affinity Capture-MS Homo sapiens
42 FAM120A 23196
Affinity Capture-MS Homo sapiens
43 RBM19 9904
Affinity Capture-MS Homo sapiens
44 FOXP1 27086
Protein-RNA Homo sapiens
45 LIN28A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CPEB1  
Affinity Capture-MS Homo sapiens
47 RB1 5925
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 EIF2AK2 5610
Affinity Capture-MS Homo sapiens
49 DDX21 9188
Affinity Capture-MS Homo sapiens
50 LIN28B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TGM2 7052
Co-fractionation Homo sapiens
52 MEPCE 56257
Affinity Capture-MS Homo sapiens
53 RPS10 6204
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
54 RRP9 9136
Affinity Capture-MS Homo sapiens
55 SRSF3 6428
Affinity Capture-MS Homo sapiens
56 YBX2 51087
Affinity Capture-MS Homo sapiens
57 SRSF6 6431
Affinity Capture-MS Homo sapiens
58 PURG  
Affinity Capture-MS Homo sapiens
59 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
60 RPL35 11224
Affinity Capture-MS Homo sapiens
61 NTRK1 4914
Affinity Capture-MS Homo sapiens
62 FGF8  
Affinity Capture-MS Homo sapiens
63 IFI16 3428
Affinity Capture-MS Homo sapiens
64 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 REXO4  
Affinity Capture-MS Homo sapiens
66 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
67 LYAR 55646
Affinity Capture-MS Homo sapiens
68 HECTD1 25831
Affinity Capture-MS Homo sapiens
69 RPLP0 6175
Affinity Capture-MS Homo sapiens
70 MAGEB2 4113
Affinity Capture-MS Homo sapiens
71 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
72 CCNT1  
Reconstituted Complex Homo sapiens
73 WDR46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 CNBP 7555
Affinity Capture-MS Homo sapiens
75 FGFBP1 9982
Affinity Capture-MS Homo sapiens
76 ACTN1 87
Co-fractionation Homo sapiens
77 OGT 8473
Reconstituted Complex Homo sapiens
78 RPL31 6160
Affinity Capture-MS Homo sapiens
79 ABT1 29777
Affinity Capture-MS Homo sapiens
80 RC3H2  
Affinity Capture-MS Homo sapiens
81 SRSF4 6429
Affinity Capture-MS Homo sapiens
82 RFC2 5982
Co-fractionation Homo sapiens
83 ZNF71  
Affinity Capture-MS Homo sapiens
84 CUL3 8452
Affinity Capture-MS Homo sapiens
85 RPS8 6202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SRSF5 6430
Affinity Capture-MS Homo sapiens
87 RPL3 6122
Affinity Capture-MS Homo sapiens
88 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
89 LOC100132735  
Protein-RNA Homo sapiens
90 MYCN  
Affinity Capture-MS Homo sapiens
91 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
92 IL7R  
Protein-RNA Homo sapiens
93 MYC  
Affinity Capture-MS Homo sapiens
94 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
95 RAF1 5894
Two-hybrid Homo sapiens
96 SNW1 22938
Affinity Capture-MS Homo sapiens
97 DDRGK1 65992
Affinity Capture-MS Homo sapiens
98 BMI1  
Affinity Capture-MS Homo sapiens
99 H2AFB2  
Affinity Capture-MS Homo sapiens
100 ZC3H3  
Affinity Capture-MS Homo sapiens
101 RBMS2 5939
Affinity Capture-MS Homo sapiens
102 RPL19 6143
Affinity Capture-MS Homo sapiens
103 RPL13 6137
Affinity Capture-MS Homo sapiens
104 TAF6 6878
Co-fractionation Homo sapiens
105 SURF6  
Affinity Capture-MS Homo sapiens
106 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
107 RNF151  
Affinity Capture-MS Homo sapiens
108 RRS1 23212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 ZCRB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 DGCR8  
Affinity Capture-MS Homo sapiens
111 RNH1 6050
Co-fractionation Homo sapiens
112 H2AFB3  
Affinity Capture-MS Homo sapiens
113 PRR3  
Affinity Capture-MS Homo sapiens
114 MKI67  
Affinity Capture-MS Homo sapiens
115 MYL12A 10627
Co-fractionation Homo sapiens
116 ESR1  
Affinity Capture-MS Homo sapiens
117 KRR1 11103
Affinity Capture-MS Homo sapiens
118 LARP7 51574
Affinity Capture-MS Homo sapiens
119 NEIL1  
Affinity Capture-MS Homo sapiens
120 PARK2  
Affinity Capture-MS Homo sapiens
121 TRIM31  
Affinity Capture-MS Homo sapiens
122 RPL23AP32 56969
Affinity Capture-MS Homo sapiens
123 RPS6 6194
Affinity Capture-MS Homo sapiens
124 POLA1  
Co-fractionation Homo sapiens
125 RPL23A 6147
Affinity Capture-MS Homo sapiens
126 RC3H1 149041
Affinity Capture-MS Homo sapiens
127 PURB 5814
Co-fractionation Homo sapiens
128 VIM 7431
Co-fractionation Homo sapiens
129 PRMT1 3276
Affinity Capture-MS Homo sapiens
130 RPL17 6139
Affinity Capture-MS Homo sapiens
131 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PURA is involved
No pathways found





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