Gene description for SRSF4
Gene name serine/arginine-rich splicing factor 4
Gene symbol SRSF4
Other names/aliases SFRS4
SRP75
Species Homo sapiens
 Database cross references - SRSF4
ExoCarta ExoCarta_6429
Entrez Gene 6429
HGNC 10786
MIM 601940
UniProt Q08170  
 SRSF4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SRSF4
Molecular Function
    nucleotide binding GO:0000166 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    gene expression GO:0010467 TAS
    RNA splicing, via transesterification reactions GO:0000375 TAS
    RNA splicing GO:0008380 TAS
    mRNA export from nucleus GO:0006406 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA 3'-end processing GO:0031124 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA processing GO:0006397 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 TAS
    nuclear speck GO:0016607 IEA
 Experiment description of studies that identified SRSF4 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRSF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRRM1  
Invivo Homo sapiens
2 PNN 5411
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 SRSF6 6431
Two-hybrid Homo sapiens
4 HAP1  
Two-hybrid Homo sapiens
5 SFRS2IP  
Two-hybrid Homo sapiens
6 DYRK1A  
Biochemical Activity Homo sapiens
7 GEMIN2 8487
Two-hybrid Homo sapiens
View the network image/svg+xml



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