Gene description for SRSF4
Gene name serine/arginine-rich splicing factor 4
Gene symbol SRSF4
Other names/aliases SFRS4
SRP75
Species Homo sapiens
 Database cross references - SRSF4
ExoCarta ExoCarta_6429
Vesiclepedia VP_6429
Entrez Gene 6429
HGNC 10786
MIM 601940
UniProt Q08170  
 SRSF4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SRSF4
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    sequence-specific mRNA binding GO:1990825 IEA
Biological Process
    RNA splicing, via transesterification reactions GO:0000375 TAS
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA processing GO:0006397 TAS
    RNA splicing GO:0008380 TAS
    response to insulin GO:0032868 IEA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified SRSF4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRSF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 HNRNPC 3183
Co-fractionation Homo sapiens
5 RPL26L1 51121
Affinity Capture-MS Homo sapiens
6 RSBN1L  
Affinity Capture-MS Homo sapiens
7 KNOP1 400506
Affinity Capture-MS Homo sapiens
8 ZNF771  
Affinity Capture-MS Homo sapiens
9 ZBTB11  
Affinity Capture-MS Homo sapiens
10 AATF  
Affinity Capture-MS Homo sapiens
11 PAXIP1  
Affinity Capture-MS Homo sapiens
12 PTMA 5757
Cross-Linking-MS (XL-MS) Homo sapiens
13 Tra2b 20462
Two-hybrid Mus musculus
14 PPIE 10450
Affinity Capture-MS Homo sapiens
15 ZNF48  
Affinity Capture-MS Homo sapiens
16 YWHAE 7531
Affinity Capture-MS Homo sapiens
17 PRPF38A 84950
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 ENOX2  
Affinity Capture-MS Homo sapiens
19 ZC3H14  
Affinity Capture-MS Homo sapiens
20 LARP1B 55132
Affinity Capture-MS Homo sapiens
21 DHX8 1659
Affinity Capture-MS Homo sapiens
22 NGRN  
Proximity Label-MS Homo sapiens
23 RPS19 6223
Affinity Capture-MS Homo sapiens
24 CHCHD1  
Proximity Label-MS Homo sapiens
25 RBBP6 5930
Affinity Capture-MS Homo sapiens
26 CAND1 55832
Affinity Capture-MS Homo sapiens
27 SRSF2 6427
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 ENOX1  
Affinity Capture-MS Homo sapiens
29 SRSF8 10929
Affinity Capture-MS Homo sapiens
30 TMPO 7112
Affinity Capture-MS Homo sapiens
31 ZNF70  
Affinity Capture-MS Homo sapiens
32 DDX23 9416
Proximity Label-MS Homo sapiens
33 MRPS10 55173
Affinity Capture-MS Homo sapiens
34 CDK12 51755
Affinity Capture-MS Homo sapiens
35 AKAP17A  
Affinity Capture-MS Homo sapiens
36 SRSF11 9295
Affinity Capture-MS Homo sapiens
37 WHSC1 7468
Affinity Capture-MS Homo sapiens
38 DYRK1A 1859
Biochemical Activity Homo sapiens
39 DDX24 57062
Affinity Capture-MS Homo sapiens
40 GPATCH8  
Affinity Capture-MS Homo sapiens
41 CPSF4  
Affinity Capture-MS Homo sapiens
42 MRPS5 64969
Affinity Capture-MS Homo sapiens
43 FBXW7  
Affinity Capture-MS Homo sapiens
44 CPSF7 79869
Two-hybrid Homo sapiens
45 ZNF638 27332
Affinity Capture-MS Homo sapiens
46 SRRM2 23524
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
47 SNRPC 6631
Affinity Capture-MS Homo sapiens
48 ZMYND11 10771
Affinity Capture-MS Homo sapiens
49 SF3A2 8175
Affinity Capture-MS Homo sapiens
50 RBM23  
Affinity Capture-MS Homo sapiens
51 NSUN4  
Affinity Capture-MS Homo sapiens
52 Dock11  
Affinity Capture-MS Mus musculus
53 PTCD1 26024
Affinity Capture-MS Homo sapiens
54 PAK1IP1  
Affinity Capture-MS Homo sapiens
55 YBX2 51087
Affinity Capture-MS Homo sapiens
56 ERAL1  
Two-hybrid Homo sapiens
57 PATZ1  
Affinity Capture-MS Homo sapiens
58 GLE1 2733
Affinity Capture-MS Homo sapiens
59 HAP1  
Two-hybrid Homo sapiens
60 ZNF777  
Affinity Capture-MS Homo sapiens
61 SFN 2810
Affinity Capture-MS Homo sapiens
62 NOC3L 64318
Affinity Capture-MS Homo sapiens
63 MRPS7 51081
Affinity Capture-MS Homo sapiens
64 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
65 MECP2 4204
Affinity Capture-MS Homo sapiens
66 NOP16 51491
Affinity Capture-MS Homo sapiens
67 CUL1 8454
Affinity Capture-MS Homo sapiens
68 MYCN  
Affinity Capture-MS Homo sapiens
69 MRPL9 65005
Affinity Capture-MS Homo sapiens
70 MAK16  
Affinity Capture-MS Homo sapiens
71 SREK1 140890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ZNF184  
Affinity Capture-MS Homo sapiens
73 MTERF3  
Affinity Capture-MS Homo sapiens
74 CTCF  
Affinity Capture-MS Homo sapiens
75 RBM19 9904
Affinity Capture-MS Homo sapiens
76 CCDC86  
Affinity Capture-MS Homo sapiens
77 ZCRB1  
Affinity Capture-MS Homo sapiens
78 RBMX 27316
Affinity Capture-Western Homo sapiens
79 Zranb2  
Two-hybrid Mus musculus
80 MRPS30 10884
Affinity Capture-MS Homo sapiens
81 CHMP4C 92421
Affinity Capture-MS Homo sapiens
82 ZDHHC17 23390
Two-hybrid Homo sapiens
83 CLK2 1196
Affinity Capture-MS Homo sapiens
84 DDX31  
Affinity Capture-MS Homo sapiens
85 RBMXL1  
Affinity Capture-MS Homo sapiens
86 MRPL32 64983
Affinity Capture-MS Homo sapiens
87 CLK4  
Affinity Capture-MS Homo sapiens
88 SCAF1  
Affinity Capture-MS Homo sapiens
89 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
90 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
91 DDX21 9188
Affinity Capture-MS Homo sapiens
92 MRPL45 84311
Affinity Capture-MS Homo sapiens
93 CUL4B 8450
Affinity Capture-MS Homo sapiens
94 PARK2  
Affinity Capture-MS Homo sapiens
95 RBM28 55131
Affinity Capture-MS Homo sapiens
96 RPS10 6204
Affinity Capture-MS Homo sapiens
97 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
98 MEPCE 56257
Affinity Capture-MS Homo sapiens
99 TRA2A 29896
Affinity Capture-MS Homo sapiens
100 TOPORS  
Affinity Capture-MS Homo sapiens
101 SIRT7  
Affinity Capture-MS Homo sapiens
102 STAU1 6780
Affinity Capture-MS Homo sapiens
103 ACIN1 22985
Affinity Capture-MS Homo sapiens
104 SRSF6 6431
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
105 ZNF22  
Affinity Capture-MS Homo sapiens
106 UBE2I 7329
Two-hybrid Homo sapiens
107 SNRPB 6628
Affinity Capture-MS Homo sapiens
108 MAPT  
Affinity Capture-RNA Homo sapiens
109 DDX10  
Affinity Capture-MS Homo sapiens
110 BARD1 580
Affinity Capture-MS Homo sapiens
111 MRPL51 51258
Affinity Capture-MS Homo sapiens
112 NAT10 55226
Affinity Capture-MS Homo sapiens
113 IFI16 3428
Affinity Capture-MS Homo sapiens
114 U2AF2 11338
Affinity Capture-MS Homo sapiens
115 RAD18  
Affinity Capture-MS Homo sapiens
116 CENPQ  
Affinity Capture-MS Homo sapiens
117 DDX54 79039
Affinity Capture-MS Homo sapiens
118 MAGOH 4116
Affinity Capture-MS Homo sapiens
119 MRPL11 65003
Affinity Capture-MS Homo sapiens
120 TRIM71  
Affinity Capture-MS Homo sapiens
121 SRPK3  
Affinity Capture-MS Homo sapiens
122 SON 6651
Affinity Capture-MS Homo sapiens
123 NKTR  
Affinity Capture-MS Homo sapiens
124 DDX27 55661
Affinity Capture-MS Homo sapiens
125 NFX1  
Affinity Capture-MS Homo sapiens
126 PCBP2 5094
Affinity Capture-Western Homo sapiens
127 LARP1 23367
Affinity Capture-MS Homo sapiens
128 RSL1D1 26156
Affinity Capture-MS Homo sapiens
129 RRP8  
Affinity Capture-MS Homo sapiens
130 RBM5  
Affinity Capture-MS Homo sapiens
131 SFPQ 6421
Co-fractionation Homo sapiens
132 ZBTB2 57621
Affinity Capture-MS Homo sapiens
133 MRPL40 64976
Affinity Capture-MS Homo sapiens
134 BMS1  
Affinity Capture-MS Homo sapiens
135 USP42  
Affinity Capture-MS Homo sapiens
136 PRPF4B 8899
Affinity Capture-MS Homo sapiens
137 MRRF  
Proximity Label-MS Homo sapiens
138 C11orf57  
Affinity Capture-MS Homo sapiens
139 DAP3 7818
Affinity Capture-MS Homo sapiens
140 CLK3  
Affinity Capture-MS Homo sapiens
141 MRPS35 60488
Affinity Capture-MS Homo sapiens
142 RC3H2  
Affinity Capture-MS Homo sapiens
143 ZBTB24  
Affinity Capture-MS Homo sapiens
144 PNN 5411
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
145 MTX2 10651
Proximity Label-MS Homo sapiens
146 CD2BP2 10421
Affinity Capture-MS Homo sapiens
147 HTATSF1 27336
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
148 SRSF1 6426
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
149 MEX3A  
Affinity Capture-RNA Homo sapiens
150 ZNF354A  
Affinity Capture-MS Homo sapiens
151 MRPL16  
Affinity Capture-MS Homo sapiens
152 BRIX1 55299
Affinity Capture-MS Homo sapiens
153 BCAP31 10134
Cross-Linking-MS (XL-MS) Homo sapiens
154 SRRM4  
Two-hybrid Homo sapiens
155 CUL3 8452
Affinity Capture-MS Homo sapiens
156 REXO4  
Affinity Capture-MS Homo sapiens
157 SRSF5 6430
Affinity Capture-MS Homo sapiens
158 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
159 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
160 CHERP 10523
Affinity Capture-MS Homo sapiens
161 SART1 9092
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
162 ZC3H10  
Affinity Capture-MS Homo sapiens
163 NXF1 10482
Two-hybrid Homo sapiens
164 DHX57 90957
Affinity Capture-MS Homo sapiens
165 C17orf85  
Affinity Capture-MS Homo sapiens
166 ZNF512  
Affinity Capture-MS Homo sapiens
167 MRPL1  
Affinity Capture-MS Homo sapiens
168 MRPL24  
Affinity Capture-MS Homo sapiens
169 BUD13  
Affinity Capture-MS Homo sapiens
170 TADA2A  
Affinity Capture-MS Homo sapiens
171 APOBEC3F 200316
Affinity Capture-MS Homo sapiens
172 ZNF629 23361
Affinity Capture-MS Homo sapiens
173 RPS13 6207
Affinity Capture-MS Homo sapiens
174 GZF1  
Affinity Capture-MS Homo sapiens
175 DKC1 1736
Affinity Capture-MS Homo sapiens
176 HP1BP3 50809
Affinity Capture-MS Homo sapiens
177 KMT2A  
Affinity Capture-MS Homo sapiens
178 SRSF10 10772
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
179 FYTTD1  
Affinity Capture-MS Homo sapiens
180 ARGLU1  
Affinity Capture-MS Homo sapiens
181 ZFP62  
Affinity Capture-MS Homo sapiens
182 MRPL47 57129
Affinity Capture-MS Homo sapiens
183 SF3B2 10992
Affinity Capture-MS Homo sapiens
184 Srsf5 20384
Two-hybrid Mus musculus
185 TICAM1  
Two-hybrid Homo sapiens
186 RPS16 6217
Affinity Capture-MS Homo sapiens
187 U2SURP 23350
Affinity Capture-MS Homo sapiens
188 ZNF574  
Affinity Capture-MS Homo sapiens
189 RRP15  
Affinity Capture-MS Homo sapiens
190 PAXBP1  
Affinity Capture-MS Homo sapiens
191 SNCA 6622
Affinity Capture-MS Homo sapiens
192 C17orf80 55028
Proximity Label-MS Homo sapiens
193 PRDM10  
Affinity Capture-MS Homo sapiens
194 CLK1  
Affinity Capture-MS Homo sapiens
195 ZNF689  
Affinity Capture-MS Homo sapiens
196 TOE1 114034
Affinity Capture-MS Homo sapiens
197 WRNIP1 56897
Co-fractionation Homo sapiens
198 MRPL22  
Affinity Capture-MS Homo sapiens
199 SRRM1 10250
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
200 RRS1 23212
Affinity Capture-MS Homo sapiens
201 PRDM15  
Affinity Capture-MS Homo sapiens
202 MINA 84864
Affinity Capture-MS Homo sapiens
203 MRPL15 29088
Affinity Capture-MS Homo sapiens
204 PPIG 9360
Affinity Capture-MS Homo sapiens
205 YTHDC1  
Affinity Capture-MS Homo sapiens
206 ZNF445  
Affinity Capture-MS Homo sapiens
207 Srsf6 67996
Two-hybrid Mus musculus
208 SRSF12  
Affinity Capture-MS Homo sapiens
209 SNRPA 6626
Affinity Capture-MS Homo sapiens
210 MRPL28 10573
Affinity Capture-MS Homo sapiens
211 Arhgap30  
Affinity Capture-MS Mus musculus
212 THRAP3 9967
Affinity Capture-MS Homo sapiens
213 CENPO  
Affinity Capture-MS Homo sapiens
214 PRRC2B  
Two-hybrid Homo sapiens
215 NSA2  
Affinity Capture-MS Homo sapiens
216 EIF4A3 9775
Affinity Capture-MS Homo sapiens
217 TRA2B 6434
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
218 DDX58 23586
Affinity Capture-RNA Homo sapiens
219 MKI67  
Affinity Capture-MS Homo sapiens
220 LARP7 51574
Affinity Capture-MS Homo sapiens
221 RPS15 6209