Gene description for GLE1
Gene name GLE1 RNA export mediator
Gene symbol GLE1
Other names/aliases GLE1L
LCCS
LCCS1
hGLE1
Species Homo sapiens
 Database cross references - GLE1
ExoCarta ExoCarta_2733
Vesiclepedia VP_2733
Entrez Gene 2733
HGNC 4315
MIM 603371
UniProt Q53GS7  
 GLE1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GLE1
Molecular Function
    inositol hexakisphosphate binding GO:0000822 IBA
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    translation initiation factor binding GO:0031369 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    mRNA export from nucleus GO:0006406 TAS
    nucleocytoplasmic transport GO:0006913 NAS
    protein transport GO:0015031 IEA
    poly(A)+ mRNA export from nucleus GO:0016973 IBA
Subcellular Localization
    extracellular space GO:0005615 HDA
    nuclear envelope GO:0005635 IDA
    nuclear pore GO:0005643 NAS
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 IDA
    ciliary basal body GO:0036064 IDA
    nuclear pore cytoplasmic filaments GO:0044614 IBA
 Experiment description of studies that identified GLE1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GLE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUPL2 11097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ZC3H14  
Affinity Capture-MS Homo sapiens
3 NXT2  
Affinity Capture-MS Homo sapiens
4 ZNF346  
Affinity Capture-MS Homo sapiens
5 S100A6 6277
Affinity Capture-MS Homo sapiens
6 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
7 KPNB1 3837
Affinity Capture-MS Homo sapiens
8 UXT 8409
Two-hybrid Homo sapiens
9 RPS19 6223
Affinity Capture-MS Homo sapiens
10 PNMAL1  
Affinity Capture-MS Homo sapiens
11 RPS3 6188
Affinity Capture-MS Homo sapiens
12 TOP2A 7153
Affinity Capture-MS Homo sapiens
13 HEATR3 55027
Affinity Capture-MS Homo sapiens
14 LLPH  
Affinity Capture-MS Homo sapiens
15 NUP153 9972
Affinity Capture-MS Homo sapiens
16 ZBTB47  
Affinity Capture-MS Homo sapiens
17 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
18 SRP14 6727
Affinity Capture-MS Homo sapiens
19 ADARB1 104
Affinity Capture-MS Homo sapiens
20 PRKRA 8575
Affinity Capture-MS Homo sapiens
21 YBX2 51087
Affinity Capture-MS Homo sapiens
22 NXT1  
Affinity Capture-MS Homo sapiens
23 FYTTD1  
Affinity Capture-MS Homo sapiens
24 RPS3A 6189
Affinity Capture-MS Homo sapiens
25 PABPC4 8761
Affinity Capture-MS Homo sapiens
26 RPL19 6143
Affinity Capture-MS Homo sapiens
27 LIN28A  
Affinity Capture-MS Homo sapiens
28 NUP155 9631
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
29 BRIX1 55299
Affinity Capture-MS Homo sapiens
30 S100A2 6273
Affinity Capture-MS Homo sapiens
31 LIN28B  
Affinity Capture-MS Homo sapiens
32 EMD 2010
Proximity Label-MS Homo sapiens
33 RPS10 6204
Affinity Capture-MS Homo sapiens
34 MEX3C 51320
Affinity Capture-MS Homo sapiens
35 NEIL1  
Affinity Capture-MS Homo sapiens
36 PABPC1 26986
Affinity Capture-MS Homo sapiens
37 MKRN2 23609
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
38 C9orf41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RAN 5901
Affinity Capture-MS Homo sapiens
40 GPR182  
Affinity Capture-MS Homo sapiens
41 REXO4  
Affinity Capture-MS Homo sapiens
42 RPL36 25873
Affinity Capture-MS Homo sapiens
43 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
44 LYAR 55646
Affinity Capture-MS Homo sapiens
45 DDX1 1653
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
46 PABPN1 8106
Affinity Capture-MS Homo sapiens
47 HECTD1 25831
Affinity Capture-MS Homo sapiens
48 RPLP0 6175
Affinity Capture-MS Homo sapiens
49 Ube2i  
Affinity Capture-MS Mus musculus
50 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
51 FGF13  
Affinity Capture-MS Homo sapiens
52 RPL11 6135
Affinity Capture-MS Homo sapiens
53 LARP1 23367
Affinity Capture-MS Homo sapiens
54 BCLAF1 9774
Affinity Capture-MS Homo sapiens
55 FGFBP1 9982
Affinity Capture-MS Homo sapiens
56 ABT1 29777
Affinity Capture-MS Homo sapiens
57 Nup98  
Affinity Capture-MS Mus musculus
58 RPL7A 6130
Affinity Capture-MS Homo sapiens
59 XPO1 7514
Affinity Capture-MS Homo sapiens
60 GLE1 2733
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
61 SRSF4 6429
Affinity Capture-MS Homo sapiens
62 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 NPM3 10360
Affinity Capture-MS Homo sapiens
64 SRSF5 6430
Affinity Capture-MS Homo sapiens
65 RPL3 6122
Affinity Capture-MS Homo sapiens
66 NUP214 8021
Affinity Capture-MS Homo sapiens
67 DUSP16  
Affinity Capture-MS Homo sapiens
68 ZC3H11A  
Affinity Capture-MS Homo sapiens
69 LARS 51520
Affinity Capture-MS Homo sapiens
70 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
71 NXF1 10482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 NXF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
74 PRPF19 27339
Affinity Capture-MS Homo sapiens
75 Nup107  
Affinity Capture-MS Mus musculus
76 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 EPRS 2058
Affinity Capture-MS Homo sapiens
78 RNF2  
Affinity Capture-MS Homo sapiens
79 SEH1L 81929
Affinity Capture-MS Homo sapiens
80 H2AFB2  
Affinity Capture-MS Homo sapiens
81 RAE1 8480
Affinity Capture-MS Homo sapiens
82 CCDC8  
Affinity Capture-MS Homo sapiens
83 RPL13 6137
Affinity Capture-MS Homo sapiens
84 SURF6  
Affinity Capture-MS Homo sapiens
85 EIF3F 8665
Affinity Capture-Western Homo sapiens
86 ILF3 3609
Affinity Capture-MS Homo sapiens
87 IARS 3376
Affinity Capture-MS Homo sapiens
88 RRS1 23212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 KRT10 3858
Two-hybrid Homo sapiens
90 IER2 9592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ZNF48  
Affinity Capture-MS Homo sapiens
92 THRAP3 9967
Affinity Capture-MS Homo sapiens
93 UTP23  
Affinity Capture-MS Homo sapiens
94 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
95 NUP35 129401
Proximity Label-MS Homo sapiens
96 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
97 NUP107 57122
Affinity Capture-MS Homo sapiens
98 SEC61B 10952
Proximity Label-MS Homo sapiens
99 IL17B 27190
Affinity Capture-MS Homo sapiens
100 RAB7A 7879
Proximity Label-MS Homo sapiens
101 RPS6 6194
Affinity Capture-MS Homo sapiens
102 Ranbp2  
Affinity Capture-MS Mus musculus
103 KRAS 3845
Synthetic Lethality Homo sapiens
104 CCDC96  
Affinity Capture-MS Homo sapiens
105 MYC  
Affinity Capture-MS Homo sapiens
106 H1FOO 132243
Affinity Capture-MS Homo sapiens
107 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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