Gene description for THRAP3
Gene name thyroid hormone receptor associated protein 3
Gene symbol THRAP3
Other names/aliases TRAP150
Species Homo sapiens
 Database cross references - THRAP3
ExoCarta ExoCarta_9967
Entrez Gene 9967
HGNC 22964
MIM 603809
UniProt Q9Y2W1  
 THRAP3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for THRAP3
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 ISS
    transcription cofactor activity GO:0003712 IDA
    ATP binding GO:0005524 IEA
    receptor activity GO:0004872 IDA
    protein binding GO:0005515 IPI
    vitamin D receptor binding GO:0042809 NAS
    phosphoprotein binding GO:0051219 IDA
    transcription coactivator activity GO:0003713 IDA
    poly(A) RNA binding GO:0044822 IDA
    thyroid hormone receptor binding GO:0046966 IDA
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 NAS
    RNA polymerase II transcription cofactor activity GO:0001104 IDA
Biological Process
    mRNA processing GO:0006397 IEA
    intracellular steroid hormone receptor signaling pathway GO:0030518 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    androgen receptor signaling pathway GO:0030521 IDA
    positive regulation of circadian rhythm GO:0042753 IMP
    mRNA stabilization GO:0048255 IMP
    RNA splicing GO:0008380 IEA
    nuclear-transcribed mRNA catabolic process GO:0000956 IDA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IMP
    rhythmic process GO:0048511 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    transcription initiation from RNA polymerase II promoter GO:0006367 IDA
Subcellular Localization
    exon-exon junction complex GO:0035145 IDA
    extracellular exosome GO:0070062 IDA
    nucleoplasm GO:0005654 IDA
    mediator complex GO:0016592 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified THRAP3 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for THRAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLEC3B 7123
Affinity Capture-MS Homo sapiens
2 RUNX1T1  
Affinity Capture-MS Homo sapiens
3 VDR  
Affinity Capture-MS Homo sapiens
4 TRIP6  
Affinity Capture-MS Homo sapiens
5 RNPS1 10921
Affinity Capture-MS Homo sapiens
6 CDK8  
Affinity Capture-MS Homo sapiens
7 LXN  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which THRAP3 is involved
No pathways found





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